Sample Preparation, Extraction Efficiency, and Determination of Six Arsenic Species Present in Food Composites

Abstract

Several sample preparation techniques were investigated to maximize the efficiency of arsenic species extraction from food composites. The optimized method includes lyophilization of food followed by prewashing with acetone and extraction by sonication with 50/50 methanol/water. Six arsenic species were separated and quantitated using an ammonium carbonate buffer system by ion exchange chromatography coupled to inductively coupled plasma mass spectrometry. The performance of the method for speciated arsenic components was evaluated using a matrix containing high fat food composite fortified with arsenic species. A certified reference material, dogfish muscle, was used to evaluate extraction methods for total arsenic content in food composites. More than 200 food composite samples were analyzed during an 18 month period, demonstrating the reliability of the analytical method over a long time period.

Nitric Acid-based Partial-digestion Method for Selective Determination of Inorganic Arsenic in Hijiki and Application to Soaked Hijiki

“…However, since methanol is an inadequate solvent for iAs extraction 8), 9) , water alone has also been used as the solvent to extract iAs because of its high polarity 10), 11) . Moreover, attempts to utilize enzymeassisted extraction have been reported for seafood 12), 13) . Quantitative extraction from some foods has been achieved, but it has been di$cult to achieve thorough extraction from samples of seaweed, including hijiki 14) .…”

Speciation of arsenic compounds in fish and oyster tissues by capillary electrophoresis‐inductively coupled plasma‐mass spectrometry

“…Methanol-water mixtures and sometimes chloroform are often used as extracting solvents for arsenic species from biological tissues [1,[4][5][6][7][8][9][10][11][12]. Extraction of organic arsenic species from biological tissues, especially from fish tissues, has been successfully performed using agitation [5,8], sonication [1,6,7,9,11,12], microwave heating [4,11], Soxhlet-extraction [11], and accelerated solvent extraction [10]. After extraction, the most commonly employed technique for the arsenic speciation is separation of the individual species by high-performance liquid chromatography (HPLC) combined with/without hydride-generation followed by detection with inductively coupled plasma-mass spectrometry (ICP-MS) [1,4,[6][7][8][9][10][11] or atomic fluorescence spectrometry (AFS) [5,12].…”

Risk Assessment of Arsenic in Agricultural Products

“…비소오염은 암석의 침식 등의 자연적 요인과 과거 농업에서 과일 재배시 의 농약(살충제)으로 사용하거나 산업 폐기물, 광산 개발 후 방치된 휴 · 폐광산으로부터 발생하는 인위적 요인에 의해 발 생한다 (Milstein et al, 2003).…”

“…일반적으로 무기비소는 유기비소보다 독성이 강하며 무기비 소 중에서도 As(III)는 As(V)보다 10배 정도 독성이 강하고 MMA, DMA 등의 유기 비소들에 비해서는 70배 정도 독성 이 강하다 (Jeong and Kim, 2008;Milstein et al, 2003 (Munoz et al, 2002), 비 소 오염지역내 버섯류에서 유기비소인 AsC가 검출되었다는 연구보고가 있다 (Koch et al, 2000). …”