Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Medvedovici, Andrei; Bacalum, Elena; David, Víctor

doi:10.1002/bmc.4137

Cited by 40 publications

(24 citation statements)

References 206 publications

(241 reference statements)

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“…The target of green analytical chemists is to develop strategies capable to perform direct on‐site measurements or to remove (or simplify) the sample preparation step . Sample preparation is considered a key step in the whole analytical protocol since, besides being considered time‐consuming, it is one of the most critical step in terms of analyte losses and contaminations . Moreover, in most cases thermal pretreatment and large amounts of organic solvents and/or inorganic acids are usually required in this step in order to extract/purify the sample prior to its analysis.…”

Section: Sfe As Green Sample Preparation Techniquementioning

confidence: 99%

Recent applications of on‐line supercritical fluid extraction coupled to advanced analytical techniques for compounds extraction and identification

Sánchez‐Camargo

Parada-Alonso

Ibáñez

2018

J of Separation Science

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In a previous review (Sánchez-Camargo et al., J. Sep. Sci. 40 (2017) 213-227), we discussed the application of on-line supercritical fluid extraction coupled to chromatographic techniques. This review includes an update of the most recent publications (from January 2016 till June 2018) on this topic, which employs advanced analytical techniques for extracting and identifying valuable analytes. Supercritical fluid extraction has been widely recognized as a green sample preparation technique, because it is efficient, environmentally friendly, powerful, and faster, offering the possibility of direct coupling to analytical instrumental techniques. Among those techniques, supercritical fluid chromatography has experienced an innovative progression in the last 10 years, and the most recent applications of supercritical fluid extraction are coupled to this advanced analytical tool. The general principles, both methodological and instrumental of on-line supercritical fluid extraction coupled to supercritical fluid chromatography are described here. Besides, applications of the mentioned coupling for analysing biological fluids, food, soil, and botanical samples are also presented and discussed. Finally, a brief description about the very recent on-line coupling of supercritical fluid extraction to ion mobility spectrometry is presented, as well as concluding remarks about the importance of using these coupled techniques in the near future.

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Section: Sfe As Green Sample Preparation Techniquementioning

confidence: 99%

Recent applications of on‐line supercritical fluid extraction coupled to advanced analytical techniques for compounds extraction and identification

Sánchez‐Camargo

Parada-Alonso

Ibáñez

2018

J of Separation Science

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“…When the biosensor comes into contact with biological fluids, one of the most challenging drawbacks is the interference from fouling agents and chemicals present in the sample matrix, such as small metabolites, proteins, and macromolecules [ 90 , 91 , 92 ]. These problems can be overcome by pretreating samples using extraction, filtration, and derivatization methods [ 10 , 93 ]: these procedures are routinely employed for food samples but are not feasible when an online evaluation is required. The direct application of biosensors to (biological) samples is the most desirable set up.…”

Section: Discussionmentioning

confidence: 99%

Biosensors for D-Amino Acids: Detection Methods and Applications

Rosini

D’Antona

Pollegioni

2020

IJMS

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D-enantiomers of amino acids (D-AAs) are only present in low amounts in nature, frequently at trace levels, and for this reason, their biological function was undervalued for a long time. In the past 25 years, the improvements in analytical methods, such as gas chromatography, HPLC, and capillary electrophoresis, allowed to detect D-AAs in foodstuffs and biological samples and to attribute them specific biological functions in mammals. These methods are time-consuming, expensive, and not suitable for online application; however, life science investigations and industrial applications require rapid and selective determination of D-AAs, as only biosensors can offer. In the present review, we provide a status update concerning biosensors for detecting and quantifying D-AAs and their applications for safety and quality of foods, human health, and neurological research. The review reports the main challenges in the field, such as selectivity, in order to distinguish the different D-AAs present in a solution, the simultaneous assay of both L- and D-AAs, the production of implantable devices, and surface-scanning biosensors. These innovative tools will push future research aimed at investigating the neurological role of D-AAs, a vibrant field that is growing at an accelerating pace.

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“…Kinetic aspects and reaction mechanisms of the derivatization are also important in the selection of its direction (Qi et al, 2014) and they may influence statistical parameters (precision and accuracy). Derivatization can be discussed by the selectivity for a functional group where the structural modification is acting, by the type of the reagent used (whether this is a common reagent, or a new designed compound) and by the type of application including the number of samples (Medvedovici, Bacalum, & David, 2018). Another point of view is the experimental framework where the derivatization is performed, by taking into consideration that bioanalysis generally has a difficult task in the isolation and concentration of target analytes from a complex sample, based on matrix removal or analyte extraction by various techniques.…”

Section: Selection Of the Derivatization Approachmentioning

confidence: 99%

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

David

Moldoveanu

Galaon

2020

Biomedical Chromatography

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Derivatization, or chemical structure modification, is often used in bioanalysis performed by liquid chromatography technique in order to enhance detectability or to improve the chromatographic performance for the target analytes. The derivatization process is discussed according to the analytical procedure used to achieve the reaction between the reagent and the target compounds (containing hydroxyl, thiol, amino, carbonyl and carboxyl as the main functional groups involved in derivatization). Important procedures for derivatization used in bioanalysis are in situ or based on extraction processes (liquid-liquid, solid-phase and related techniques) applied to the biomatrix. In the review, chiral, isotope-labeling, hydrophobicity-tailored and post-column derivatizations are also included, based on representative publications in the literature during the last two decades. Examples of derivatization reagents and brief reaction conditions are included, together with some bioanalytical applications and performances (chromatographic conditions, detection limit, stability and sample biomatrix).

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Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Cited by 40 publications

References 206 publications

Recent applications of on‐line supercritical fluid extraction coupled to advanced analytical techniques for compounds extraction and identification

Recent applications of on‐line supercritical fluid extraction coupled to advanced analytical techniques for compounds extraction and identification

Biosensors for D-Amino Acids: Detection Methods and Applications

Derivatization procedures and their analytical performances for HPLC determination in bioanalysis

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