Sample pretreatment by UV photolysis for the ion chromatographic analysis of plant material

Buldini, Pier Luigi; Cavalli, Silvano; Mevoli, Anna

doi:10.1016/0021-9673(96)00035-0

Cited by 39 publications

(8 citation statements)

References 6 publications

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“…Uma outra tendência que se desenvolveu a partir do renovado interesse por sistemas de irradiação em fluxo, foi o acoplamento com etapas de separação por cromatografia líquida. Buldini et al 36 propuseram um procedimento para determinar Cl, Br, P (como PO 4 3-), S (como SO 4 2-), Pb, Cd, Fe, Cu, Ni, Zn e Co totais em folhas, por cromatografia de íons após digestão UV (lâmpada de 500 W, 85 ºC). Tsalev et al 37,38 , Ebdon et al…”

Section: Decomposição De Matrizes Orgânicas Por Irradiação Uvunclassified

O uso de radiação ultravioleta para o pré-tratamento de amostras em análise inorgânica

Cavicchioli¹,

Gutz²

2003

Quím. Nova

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. Prof. Lineu Prestes, 748, 05508-900 São Paulo -SP Recebido em 28/11/02; aceito em 15/4/03 USE OF UV RADIATION FOR SAMPLE PRETREATMENT IN INORGANIC ANALYSIS. The basic principles of UV irradiation as sample pre-treatment step and its potential for inorganic analysis are illustrated and discussed through significant examples from the literature. Not only does this overview cover the classical applications of this technique in the decomposition of organic matter in electroanalysis, but it also presents recent trends, including the increasing interest in employing UV irradiation in flow analytical systems, successful attempts to enhance its effectiveness and the coupling with chromatographic and spectroscopic methodologies. Furthermore, a number of relevant cases of UV-driven derivatization reactions involving photo-sensitive inorganic species are presented, showing some convenient options to perform fast and reliable determination of inorganic and organic analytes.Keywords: UV radiation; sample pretreatment; inorganic analysis. INTRODUÇÃOCom a expressão pré-tratamento de amostra se indica o conjunto dos procedimentos necessários para converter física e quimicamente uma amostra em uma forma que permita efetuar, dentre as limitações impostas pela natureza e a morfologia da mesma, a determinação do(s) analito(s) e realizar sua quantificação o quanto mais precisa e exata [1][2][3][4] . Considerando o vasto panorama da química analítica, em particular a multiplicidade de tipos de amostras e toda a ampla gama de técnicas de detecção, sem contar os inúmeros possíveis analitos, a etapa de pré-tratamento da amostra pode incluir os mais diversos processos, freqüentemente vários deles em seqüências que, amiúde, dão origem a procedimentos integrais bastante demorados e complexos. A título de exemplo, veja-se o recente trabalho de Hanway et al.5 sobre um procedimento para a quantificação de adutos benzenocisteína presentes nas cadeias protéicas da hemoglobina (a globina), apontados como marcadores biológicos da exposição da população a hidrocarbonetos aromáticos. O tempo para realizar a detecção por GC-MS constitui cerca de 0,2% da duração total do ensaio, que inclui as etapas de diálise, precipitação e purificação da proteína, hidrólise, purificação por extração em fase sólida, duas separações por HPLC e finalmente a derivatização para o derivado sililado, pré-via à injeção no cromatógrafo para gases. Trata-se de exemplo extremo, mas bastante emblemático, da variedade de possíveis processos envolvidos e da relevância do preparo da amostra para alcançar objetivos analíticos às vezes árduos.De fato, porém, para simplificar a questão, não é impróprio afirmar que as operações de preparo da amostra se restringem, na quase totalidade, a poucas classes de operações: solubilização, transferên-cia de meio, pré-concentração/diluição, purificação, derivatização. A solubilização de matrizes sólidas é freqüente em amostras como alimentos, sedimentos, tecidos biológicos, solos, rochas, etc.. A transferência de analitos de um meio para out...

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Section: Decomposição De Matrizes Orgânicas Por Irradiação Uvunclassified

O uso de radiação ultravioleta para o pré-tratamento de amostras em análise inorgânica

Cavicchioli¹,

Gutz²

2003

Quím. Nova

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“…Sample B contained a relatively high concentration of Hg 2+ that could be detected. Many references [2,5,13,17] have determined heavy and transition metals by spiking and have got good results. So in this study, Hg 2+ was determined by using Sample A spiked with 0.5 mg Hg 2+ and Sample B spiked with 0.4 mg l −1 Hg 2+ .…”

Section: Samplesmentioning

confidence: 99%

“…But ion chromatography (IC) seems to be one of the most effective and simple techniques to determine both anionic and cationic species owing to its high sensitivity, rapidity, selectivity and simplicity coupled with the possible advantage of simultaneous determination [2]. The widely used cationic IC methods involve a cation-exchange (or a mixed cation-and anion-exchange) separation followed by post-column complexation with 4-(2-pyridylazo)resorcinol (PAR), to form chelates which can be sensitively monitored by spectrophotometric detection [3].…”

Section: Introductionmentioning

confidence: 99%

Ion chromatography of heavy and transition metals by on- and post-column derivatizations

Ding

Mou

Liu

et al. 2000

Analytica Chimica Acta

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A new method for the simultaneous separation and determination of mercury(II) with other heavy and transition metals by ion chromatography (IC) was developed. This method involves on-column derivatization with 2,6-pyridinedicarboxylic acid (PDCA) and anion-exchange separation, followed by a post-column reaction with 2-[(5-bromo-2-pyridyl)-azo]-5-diethylaminophenol (5-Br-PADAP) to form metal-5-Br-PADAP chelates, which can be sensitively monitored by spectrophotometric detection at 565 nm. PDCA was first combined with Na 2 C 2 O 4 to separate the metals within 16 min. The optimum separation and derivatization conditions were studied in detail. The detection limits of Pb 2+ , Cu 2+ , Ni 2+ , Zn 2+ , Co 2+ , Cd 2+ , Mn 2+ and Hg 2+ were 0.2 mg l −1 (115 l loop), 1.5, 5, 0.5, 5, 5, 3 and 20 g l −1 (676 l loop), respectively. This method was applied to the analysis of standard rice sample, waste cellpacking and municipal solid waste fly ash with satisfying results.

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“…The widely used IC methods for determining transition-metal ions are based on a cation or bifunctional exchange separation, followed by post-column complexation with 4-(2-pyridylazo)resorcinol (PAR) to form chelates monitored by spectrophotometric detection. [1][2][3][4][5][6][7] Some of them show good sensitivity and selectivity. However, a postcolumn reactor between the separator column and the UV-Vis detector is required for the complex, which results in a long reaction time and complicated equipment.…”

Section: Introductionmentioning

confidence: 99%

“…However, a postcolumn reactor between the separator column and the UV-Vis detector is required for the complex, which results in a long reaction time and complicated equipment. [5][6][7] The chemiluminescence (CL) analysis of transition-metal ions in recent years has received increasing attention for its high sensitivity, simple instrumentation, wide dynamic range and good reproducibility. However, CL methods often suffer from low selectivity because many coexisting substances can react with luminol or other CL reagents to give light emission.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Copper(II) and Cobalt(II) by Ion Chromatography Coupled with Chemiluminescent Detection

Lin

Huie

2003

ANAL. SCI.

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The pollution of water with transition-metal ions has increased dramatically in recent years as a consequence of the expansion of industrial activities. Transition-metal ions are toxic when their concentrations in water exceed certain levels. Therefore, a rapid and convenient method for their simultaneous analysis in natural water samples has attracted much attention.Ion chromatography (IC) has been successfully used to separate transition metal-ions. The widely used IC methods for determining transition-metal ions are based on a cation or bifunctional exchange separation, followed by post-column complexation with 4-(2-pyridylazo)resorcinol (PAR) to form chelates monitored by spectrophotometric detection.1-7 Some of them show good sensitivity and selectivity. However, a postcolumn reactor between the separator column and the UV-Vis detector is required for the complex, which results in a long reaction time and complicated equipment. 5-7The chemiluminescence (CL) analysis of transition-metal ions in recent years has received increasing attention for its high sensitivity, simple instrumentation, wide dynamic range and good reproducibility. However, CL methods often suffer from low selectivity because many coexisting substances can react with luminol or other CL reagents to give light emission. Therefore, the coupling of the highly sensitive detection of CL with the high-efficiency separation of IC can improve the selectivity of CL detection. The cause limiting its widespread application was the compatibility of IC separation and CL detection. If successful, it would maintain a good separation efficiency of IC, and provide a high sensitivity of CL. To the best of our knowledge, almost all post-column CL assays for trasition-metal ions have been based on a metal-catalyzed reaction of luminol with hydrogen peroxide (H2O2). [8][9][10][11][12] Therefore, it is highly desirable to develop a new CL system in the absence of any special CL reagents for the simultaneous determination of transition-metal ions.The decomposition of H2O2 catalyzed by transition-metal ions or their complexes in homogeneous and heterogeneous systems has been extensively studied, and different mechanisms have been suggested for these reactions. [13][14][15][16][17] It is well known that the decomposition of H2O2 in the presence of transition-metal ions produces singlet oxygen ( 1 O2), which is a light-emitting species. 18 Although the CL emission from the decomposition of H2O2 has been recognized for a long time, and many CL systems using H2O2 as an oxidant have been reported, 19-21 the application of the decomposition of H2O2 catalyzed by transition-metal ions without any special CL reagents is rare. In our previous work, 22 we developed a Cu(II)-H2O2-amino acids system for the direct determination of tryptophan and phenylalanine after liquid-chromatographic separation with water alone as the eluent.In the present work, based on the decomposition of H2O2 catalyzed by transition-metal ions, such as Cu(II) and Co(II), in a basic aqueous solution to...

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Sample pretreatment by UV photolysis for the ion chromatographic analysis of plant material

Cited by 39 publications

References 6 publications

O uso de radiação ultravioleta para o pré-tratamento de amostras em análise inorgânica

O uso de radiação ultravioleta para o pré-tratamento de amostras em análise inorgânica

Ion chromatography of heavy and transition metals by on- and post-column derivatizations

Simultaneous Determination of Copper(II) and Cobalt(II) by Ion Chromatography Coupled with Chemiluminescent Detection

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