Sample-pretreatment-free based high sensitive determination of aflatoxin M<sub>1</sub>in raw milk using a time-resolved fluorescent competitive immunochromatographic assay

Tang, Xiaoqian; Zhang, Zhaowei; Li, Peiwu; Zhang, Qi; Jiang, Jun; Wei, Du; Lei, Jiawen

doi:10.1039/c4ra12097c

Cited by 38 publications

(23 citation statements)

References 22 publications

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“…Europium, present on most of UPTs, emits fluorescence when reduced from Eu 3 þ to Eu 2 þ , so several researchers have exploited that property and combined europium with microparticles (Rundström et al, 2007) and silica beads (Xia et al, 2009;Tang et al, 2015;Zhang et al, 2014) to use them as transducers of LFS. Although the results are satisfying, QDs and UPTs exhibit better limits of detection and working ranges.…”

Section: Other Fluorescent Materialsmentioning

confidence: 98%

Nanoparticle-based lateral flow biosensors

Quesada-González

Merkoçi

2015

Biosensors and Bioelectronics

512

293

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Lateral flow biosensors (LFBs) are paper-based devices which permit the performance of low-cost and fast diagnostics with good robustness, specificity, sensitivity and low limits of detection. The use of nanoparticles (NPs) as labels play an important role in the design and fabrication of a lateral flow strip (LFS). The choice of NPs and the corresponding detection method directly affect the performance of these devices. This review discusses aspects related to the application of different nanomaterials (e.g. gold nanoparticles, carbon nanotubes, quantum dots, up-converting phosphor technologies, and latex beads, between others) in LFBs. Moreover, different detection methods (colorimetric, fluorescent, electrochemical, magnetic, etc.) and signal enhancement strategies (affording secondary reactions or modifying the architecture of the LFS) as well as the use of devices such as smartphones to mediate the response of LFSs will be analyzed.

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Section: Other Fluorescent Materialsmentioning

confidence: 98%

Nanoparticle-based lateral flow biosensors

Quesada-González

Merkoçi

2015

Biosensors and Bioelectronics

512

293

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“…A linear relationship between the fluorescence recovery of FAM and the concentration of AFM 1 in the range from 5 pg/mL to 150 pg/mL was obtained in the HEPES buffer, with the detection limit of 1.5 pg/mL (calculated as the concentration corresponding to three times of the standard deviation of the background signal from seven independent measurements) ( Figure 3 b). Compared to the previously reported structure, switching aptamer-based FRET assay for AFM 1 detection with a linear detection range from 25 ng/kg to 2000 ng/kg [ 25 ], and the time-resolved fluorescent competitive immunochromatographic assay for AFM 1 detection in milk with a linear dynamic range of 0.1–2.0 ng/mL [ 34 ], the sensitivity of the present sensor is significantly improved, which shows great potential to detect lower concentration of AFM 1 in milk samples. The performance improvement of this FRET largely relied on the excellent fluorescence quenching ability of PdNPs towards FAM, with almost negligible non-specific fluorescence quenching.…”

Section: Resultsmentioning

confidence: 99%

Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk

Yang

et al. 2017

Toxins

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A highly sensitive aptasensor for aflatoxin M1 (AFM1) detection was constructed based on fluorescence resonance energy transfer (FRET) between 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). PdNPs (33 nm) were synthesized through a seed-mediated growth method and exhibited broad and strong absorption in the whole ultraviolet-visible (UV-Vis) range. The strong coordination interaction between nitrogen functional groups of the AFM1 aptamer and PdNPs brought FAM and PdNPs in close proximity, which resulted in the fluorescence quenching of FAM to a maximum extent of 95%. The non-specific fluorescence quenching caused by PdNPs towards fluorescein was negligible. After the introduction of AFM1 into the FAM-AFM1 aptamer-PdNPs FRET system, the AFM1 aptamer preferentially combined with AFM1 accompanied by conformational change, which greatly weakened the coordination interaction between the AFM1 aptamer and PdNPs. Thus, fluorescence recovery of FAM was observed and a linear relationship between the fluorescence recovery and the concentration of AFM1 was obtained in the range of 5–150 pg/mL in aqueous buffer with the detection limit of 1.5 pg/mL. AFM1 detection was also realized in milk samples with a linear detection range from 6 pg/mL to 150 pg/mL. The highly sensitive FRET aptasensor with simple configuration shows promising prospect in detecting a variety of food contaminants.

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“…The FRET technique was applied by several researchers, allowing detection of different mycotoxins (e.g., AFM1, AFB1, OTA and FB1) in foods (e.g., milk, peanuts, rice, and/or maize). Antibodies or aptamers were immobilized onto fluorescent nanoparticles-graphene oxide, quantum dots-AuNPs or nanogold-strips, forming thin films and enabling achieving low detection limits (e.g., 0.02 to 0.1 ng·mL −1 , depending on the method/mycotoxin) [80,87,88].…”

Section: Fluorescence Sensorsmentioning

confidence: 99%

Thin Films Sensor Devices for Mycotoxins Detection in Foods: Applications and Challenges

et al. 2019

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Mycotoxins are a group of secondary metabolites produced by different species of filamentous fungi and pose serious threats to food safety due to their serious human and animal health impacts such as carcinogenic, teratogenic and hepatotoxic effects. Conventional methods for the detection of mycotoxins include gas chromatography and high-performance liquid chromatography coupled with mass spectrometry or other detectors (fluorescence or UV detection), thin layer chromatography and enzyme-linked immunosorbent assay. These techniques are generally straightforward and yield reliable results; however, they are time-consuming, require extensive preparation steps, use large-scale instruments, and consume large amounts of hazardous chemical reagents. Rapid detection of mycotoxins is becoming an increasingly important challenge for the food industry in order to effectively enforce regulations and ensure the safety of food and feed. In this sense, several studies have been done with the aim of developing strategies to detect mycotoxins using sensing devices that have high sensitivity and specificity, fast analysis, low cost and portability. The latter include the use of microarray chips, multiplex lateral flow, Surface Plasmon Resonance, Surface Enhanced Raman Scattering and biosensors using nanoparticles. In this perspective, thin film sensors have recently emerged as a good candidate technique to meet such requirements. This review summarizes the application and challenges of thin film sensor devices for detection of mycotoxins in food matrices.

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Sample-pretreatment-free based high sensitive determination of aflatoxin M₁in raw milk using a time-resolved fluorescent competitive immunochromatographic assay

Abstract: A highly-sensitive TRFICA was developed to detect AFM1 in raw milk samples within 6 minutes without any sample pretreatments.

Cited by 38 publications

References 22 publications

Nanoparticle-based lateral flow biosensors

Nanoparticle-based lateral flow biosensors

Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk

Thin Films Sensor Devices for Mycotoxins Detection in Foods: Applications and Challenges

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Sample-pretreatment-free based high sensitive determination of aflatoxin M1in raw milk using a time-resolved fluorescent competitive immunochromatographic assay

Abstract: A highly-sensitive TRFICA was developed to detect AFM1 in raw milk samples within 6 minutes without any sample pretreatments.

Cited by 38 publications

References 22 publications

Nanoparticle-based lateral flow biosensors

Nanoparticle-based lateral flow biosensors

Palladium Nanoparticles-Based Fluorescence Resonance Energy Transfer Aptasensor for Highly Sensitive Detection of Aflatoxin M1 in Milk

Thin Films Sensor Devices for Mycotoxins Detection in Foods: Applications and Challenges

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Sample-pretreatment-free based high sensitive determination of aflatoxin M₁in raw milk using a time-resolved fluorescent competitive immunochromatographic assay