Sample size estimation

Likhvantsev, V.V.; Yadgarov, M.Ya.; Берикашвили, Л.Б.; Каданцева, К. К.; Kuzovlev, Аrtem

doi:10.17116/anaesthesiology202006177

Cited by 17 publications

(1 citation statement)

References 16 publications

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“…In recent years, the European Union has developed approaches that allow us to study pathological processes in animals, maximally limiting the number of animals involved in the experiment (OECD protocols 420, 423, 425) [ 25 , 26 , 27 ]. Therefore, to determine the sample size, we were guided by recommendations that are the result of many years of work by specialized specialists [ 28 , 29 , 30 ]. It was decided to limit the number of animal specimens to 22 included in the experiment.…”

Section: Methodsmentioning

confidence: 99%

A New Way to Model Periodontitis in Laboratory Animals

Moiseev,

Donskov,

Dubrovin

et al. 2023

Dentistry Journal

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The prevalence of periodontal diseases is increasing, tends to increase with age and is considered as one of the main causes of tooth loss. To assess the effectiveness of new methods of treatment of periodontal diseases, studies on laboratory animals can be promising. The aim of the study: to develop a new method of accelerated modeling of experimental periodontitis on laboratory animals. Material and methods. The study was carried out on 22 female rats. A wire ligature was applied to the cervical area of the incisors of the animals in an eight-shaped manner. Plaque obtained from a patient with periodontitis was placed under the wire, and nicotine and ethyl alcohol solutions were injected under the gingival mucosa. A complex index has been proposed to assess inflammation. At the end of the experiment the animals were euthanized, their jaws were dissected into dentoalveolar blocks and further descriptive histologic analysis was performed. Results. On the second day the gingiva of the rats acquired a cyanotic-pink color, on the fourth day the consistency of the gingiva became friable, mobility appeared in the lower incisors. Complex index of inflammation in animals of the main group: before the study—9, on the 7th day—195. Gingival preparations showed signs of exudative inflammation. In alveolar processes—irreversible resorption of bone structures. The difference of indicators in animals before and after the experiment was statistically significant (p < 0.05). Conclusion. The new experimental model of periodontitis is reproduced in a short period of time, provides intensive development of inflammation, leads to disruption of the integrity of epithelial and connective tissue attachment, destruction of alveolar bone.

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Section: Methodsmentioning

confidence: 99%

A New Way to Model Periodontitis in Laboratory Animals

Moiseev,

Donskov,

Dubrovin

et al. 2023

Dentistry Journal

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An Example of Diagnostics of a Technological Cutting System When Milling Materials of High Hardness

Mokritskii,

Kosmynin,

Skripilev

et al. 2023

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There is a growing demand for materials that have a layer of high hardness applied on a soft base. Technologies for obtaining such materials have been developed. One of the technologies is based on the use of surfacing from specialized powder wire. The hardness of such a surfacing is HRC 65. Accordingly, there are difficulties in processing such surfacing. The paper considers the features of milling such surfacings with a face mill with mechanical fastening of replaceable carbide plates. The purpose of the work is to improve the efficiency of the milling cutters and to identify a method by which it is convenient and reliable to diagnose the condition of the cutting plates of the milling cutter. The paper shows that the goal is achieved by using the acoustic emission method, in particular by using the parameter "total count of the number of acoustically active events".

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Contribution of <i>AGTR</i> 1 Promoter Region Polymorphism to the Progression and Outcome of Sepsis in Patients with Various Comorbidities

Чумаченко¹,

Григорьев²,

Писарев³

2021

Obŝaâ reanimatologiâ

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Blood pressure dysregulation and circulatory failure are major contributors to the progression of sepsis and especially septic shock. One of the genes affecting the vascular endothelium and arteriolar tone is the angiotensin II receptor 1 gene (AGTR1). The AGTR1 rs275651 single-nucleotide polymorphism is associated with the development of angina, high altitude pulmonary edema, and hypertension. The significance of the AGTR1 rs275651 polymorphism in sepsis, particularly in patients with significant comorbidity, has not been studied previously.The aim of the study was to determine the impact of AGTR1 functional polymorphism on sepsis outcome in patients with various comorbidities, including cardiovascular disease and type 2 diabetes mellitus.Material and methods. A prospective study included 144 ICU patients of two clinical hospitals in Moscow, aged 18-75 years with clinical signs of sepsis (Sepsis-3, 2016).Results. In the group of patients with cardiovascular diseases, carriers of the TT AGTR1 rs275651 genotype had a lower mortality rate compared with carriers of the A allele (25 deaths out of 33 versus 16 out of 16, respectively, P=0.041, Fisher's exact test; P=0.0019, log-rank test). In the group of patients with diabetes mellitus (n=62), we also found significant differences in sepsis outcome based on the AGTR1 rs275651 genotype variant. The subgroup of TT AGTR1 rs275651 genotype carriers demonstrated significantly lower mortality compared with TA, AA genotypes carriers (27 deaths out of 41 and 20 out of 21, respectively, P=0.012, Fisher's exact test; OR=10.37; 95% CI: 1.26 to 85.5; P<0.0001, log-rank test).Conclusion. We found an association of the functional polymorphism AGTR1 -777 T>A (rs275651) with sepsis outcome in ICU patients with high-value baseline comorbidity: carriers of the more common TT genotype had lower mortality compared to carriers of the minor A allele.

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Sample size estimation

Cited by 17 publications

References 16 publications

A New Way to Model Periodontitis in Laboratory Animals

A New Way to Model Periodontitis in Laboratory Animals

An Example of Diagnostics of a Technological Cutting System When Milling Materials of High Hardness

Contribution of <i>AGTR</i> 1 Promoter Region Polymorphism to the Progression and Outcome of Sepsis in Patients with Various Comorbidities

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