Sample-solvent-induced peak broadening in the reversed-phase high-performance liquid chromatography of Aspirin and related analgesics

Williams, Kevan; Po, A. Li Wan; Irwin, W. J.

doi:10.1016/s0021-9673(00)87299-4

Cited by 47 publications

(4 citation statements)

References 8 publications

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“…Validation of the method UHPLC optimization parameters In reversed-phase HPLC, peak deformation, peak area variation, and reduced column efficiency, which are known as solvent effects, may occur when the ability of the sample solvent to dissolve the sample is stronger than that of the mobile phase. 30,31 When acetonitrile was the sample solvent, there was a problematic solvent effect that led to splitting of the carbendazim peak (Fig. 1(a)).…”

Section: Resultsmentioning

confidence: 99%

Dissipation and dietary risk assessment of carbendazim and epoxiconazole in citrus fruits in China

et al. 2021

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BACKGROUND Carbendazim and epoxiconazole are widely applied to control anthracnose and sand bark fungal diseases in citrus. The residues of these two fungicides in citrus and their potential risk to consumers have generated much public concern. We therefore sought to investigate the dissipation, residue, and dietary risk assessment of carbendazim and epoxiconazole in citrus. RESULTS The dissipation kinetics and residue levels of carbendazim and epoxiconazole in citrus under field conditions were measured using dispersive solid‐phase extraction and ultra‐high‐performance liquid chromatography–tandem mass spectrometry. The citrus samples were extracted with acetonitrile and purified by primary secondary amine sorbent. The mean recoveries of carbendazim and epoxiconazole ranged from 86.2 to 105.6% and relative standard deviations were ≤9.8%. The half‐lives of carbendazim and epoxiconazole in whole citrus ranged from 2.0 to 18.0 days. Hazard quotient (HQ) and risk quotient (RQ) models were applied to whole citrus for dietary exposure risk assessment based on the terminal residue test. Hazard quotients ranged from 0.066 to 0.134% and RQs from 18.48 to 82.12%. CONCLUSION Carbendazim and epoxiconazole in citrus degraded rapidly following first‐order kinetics models. The dietary risk of exposure to both carbendazim and epoxiconazole through citrus, based on HQ and RQ, was acceptable for human consumption. This study indicates scientifically validated maximum residue limits in citrus, which are currently lacking for epoxiconazole in China. © 2021 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Dissipation and dietary risk assessment of carbendazim and epoxiconazole in citrus fruits in China

et al. 2021

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“…In RP‐HPLC, when the elution ability of the solvent to dissolve the sample (sample solvent) is stronger than that of the mobile phase, solvent effects may occur, such as peak deformation, peak area variation, and reduced column efficiency . Thus, we investigated sample solvents consisting of methanol and water.…”

Section: Resultsmentioning

confidence: 99%

Ultra high performance liquid chromatography with tandem mass spectrometry method for determining dinotefuran and its main metabolites in samples of plants, animal‐derived foods, soil, and water

Zhang

Duan

et al. 2018

J of Separation Science

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An ultra high-performance liquid chromatography with tandem triple quadrupole mass spectrometry residue method was developed and validated for the quantification and identification of dinotefuran and its main metabolites 1-methyl-3-(tetrahydro-3-furylmethyl) urea and 1-methyl-3-(tetrahydro-3-furylmethyl) guanidine in fruit (watermelon), vegetable (cucumber), cereal (rice), animal-derived foods (milk, egg, and pork), soil, and water. The samples were extracted with acetonitrile containing 15% v/v acetic acid and purified with dispersive solid-phase extraction with octadecylsilane, primary secondary amine, graphitized carbon black, or zirconia-coated silica prior to analysis. The method had an excellent linearity (R ≥ 0.9942, 1-500 μg/L) and satisfactory recoveries (73-102%) at five spiked levels (0.001, 0.01, 0.05, 0.5, and 2 mg/kg) with intra- or interday precision in the range of 0.8-9.5% and 3.0-12.8% for the three compounds in the eight matrices. The limits of quantification were 10 μg/kg for 1-methyl-3-(tetrahydro-3-furylmethyl) guanidine and 1 μg/kg for 1-methyl-3-(tetrahydro-3-furylmethyl) urea and dinotefuran. The applicability of the developed method was demonstrated by determining the occurrence of dinotefuran, 1-methyl-3-(tetrahydro-3-furylmethyl) guanidine, and 1-methyl-3-(tetrahydro-3-furylmethyl) urea in various samples from plants, animal-derived foods, and the environment. From 80 samples, 70 contained dinotefuran (0.8-11.7 μg/kg), among which six also contained 1-methyl-3-(tetrahydro-3-furylmethyl) urea (water and rice, 0.5-0.9 μg/kg).

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“…The best solution is to dissolve the sample using the mobile phase or similar solvents. [16][17][18] It is not convenient to change the sample solvent in the protein precipitation pretreatment method; therefore a weak elution intensity solvent was selected to dilute the supernatant. The supernatant obtained by using the protein precipitant was mixed with puried water in the initial proportion of the mobile phase, and centrifuged for injection into the LC-MS/MS system.…”

Section: Optimization Of Sample Processing Proceduresmentioning

confidence: 99%

A liquid chromatography-tandem mass spectrometry method for the quantitation of imidafenacin in human plasma: application to a clinical pharmacokinetic study

Shen

Wang

et al. 2016

Anal. Methods

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Sample-solvent-induced peak broadening in the reversed-phase high-performance liquid chromatography of Aspirin and related analgesics

Cited by 47 publications

References 8 publications

Dissipation and dietary risk assessment of carbendazim and epoxiconazole in citrus fruits in China

Dissipation and dietary risk assessment of carbendazim and epoxiconazole in citrus fruits in China

Ultra high performance liquid chromatography with tandem mass spectrometry method for determining dinotefuran and its main metabolites in samples of plants, animal‐derived foods, soil, and water

A liquid chromatography-tandem mass spectrometry method for the quantitation of imidafenacin in human plasma: application to a clinical pharmacokinetic study

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