Sampling Blood from the Lateral Tail Vein of the Rat

Lee, Graham; Goosens, Ki A.

doi:10.3791/52766

Cited by 61 publications

(40 citation statements)

References 12 publications

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“…A 150-ml blood sample was withdrawn from the jugular vein with a fraction collector according to a programmed schedule at 5, 15, 30, 45, and 60 min and 1.5, 2, 2.5, and 3 h following drug administration. The total blood volume of rodents is approximately 7% of their body weight, and approximately 20% of total blood volume can be withdrawn (Lee and Goosens, 2015). Thus, the volume of a blood sample that can be collected from a Sprague-Dawley rat is approximately 19.6 ml.…”

Section: Experimental Animalsmentioning

confidence: 99%

Local Irradiation Modulates the Pharmacokinetics of Metabolites in 5-Fluorouracil—Radiotherapy–Pharmacokinetics Phenomenon

Liu

Tsai²,

Chen

et al. 2020

Front. Pharmacol.

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Background: The effects of radiotherapy (RT) on the pharmacokinetics (PK) of 5-FU and 5-fluoro-5,6-dihydro-uracil (5-FDHU) were investigated by animal experiments. Methods: Whole-pelvis RT with 0.5 and 2 Gy was delivered to Sprague-Dawley rats. 5-FU at 100 mg/kg was intravenously infused 24 h after radiation. The pharmacokinetics of 5-FU and 5-FDHU in the plasma and bile system were calculated. Results: The areas under the concentration versus time curve (AUC) of 5-FU in the plasma were reduced by local irradiation by 23.7% at 0.

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Section: Experimental Animalsmentioning

confidence: 99%

Local Irradiation Modulates the Pharmacokinetics of Metabolites in 5-Fluorouracil—Radiotherapy–Pharmacokinetics Phenomenon

Liu

Tsai²,

Chen

et al. 2020

Front. Pharmacol.

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“…Assay of fasting blood glucose was performed in the manner described by Lee and Goossens [17]. Briefly blood was sampled from the lateral tail vein after a six hour fast.…”

Section: Assay Of Fasting Blood Glucosementioning

confidence: 99%

Hepatoprotective effects ofErythrina abyssinicaLam Ex Dc against Non Alcoholic Fatty Liver Disease in Sprague Dawley Rats

Yenesew

et al. 2019

Preprint

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Background: Non-alcoholic fatty liver disease (NFLD) is the hepatic manifestation of the metabolic syndrome recognized as the most prevalent chronic liver disease across all age groups. NFLD is strongly associated with obesity, insulin resistance, hypertension and dyslipidemia. Extensive research efforts are geared, through pharmacological approach, towards preventing or reversing this. Erythrina abyssinica Lam ex DC is an indigenous tree used widely used in traditional medicine, including for the treatment of liver related diseases, and has been shown to possess hypoglycemic, anti-oxidant, antimicrobial and anti-plasmodia effects. The present study is aimed at establishing the effects of E. abyssinica on the development of Non-alcoholic fatty liver disease induced by a high-fat and high-sugar diet in rats, in-vivo model. (40) were randomly divided into five groups: positive control (pioglitazone), Negative control (high fat/high sugar diet), low test dose (200 mg/kg), high test dose (400 mg/kg) and normal group (standard chow pellets and fresh water). Methods: Forty ratsThe inhibitory effect of the stem bark extract of E. abyssinica on the development of NAFLD was evaluated by chronic administration the herb extracts to rats on a high-fat/high-sugar diet.Biochemical indices of hepatic function including serum lipid profile, serum aspartate transaminase and alanine transaminase levels were then determined. Histological analysis of liver samples was carried out to quantify the degree of steato-hepatitis. Liver weights were taken and used to determine the hepatic index. The data was analyzed using one-way ANOVA, and Tukey's post-hoc tests were done in cases of significance. Histology data was analyzed using Kruskal-Wallis and Dunn's post-hoc test was done in cases of significance. Significance was set at p<0.05. Results:The freeze dried extract of E. abyssinica had significant effects on fasting blood glucose [5.43 ± 0.17 (HF/HSD) vs 3.8 ± 0.15 (E 400 mg/kg) vs 4.54 ± 0.09 (E 200 mg/kg) vs. 4.16 ± 0.13 (PIOG) vs. 2.91 ± 0.16 (normal control): P value < 0.0001], and insulin sensitivity [329.4 ± 13.48 mmol/L · min (HF/HSD) vs. 189.8 ± 12.11 mmol/L · min (E 400 mg/kg) vs. 233.8 ± 6.55 mmol/L · min (E 200 mg/kg) vs. 211.1 ± 7.35 mmol/L · min (PIOG) vs. 142.9 ± 11.94 mmol/L · min: P value < 0.0001],The extract had significant effects on hepatic indices including, hepatic triglycerides (P value < 0.0001), liver weights (P value < 0.0001), liver weight-body weight ratio (P value < 0.0001), serum ALT levels (P value < 0.0001), serum AST (P value < 0.0017), serum total cholesterol (P value < 0.0001), serum triglycerides (P value < 0.0001), and serum LDL-cholesterol (P value < 0.0001). The extracts however showed no significant effects on HDL-cholesterol (P value = 0.4759).Histological analysis showed that the extract appears to possess protective effects against steatosis, inflammation and hepatic ballooning, with the high dose (400mg/kg) being more hepatoprotective. Conclusion:The freeze dried stem bark extract of Eryth...

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“…The uniformity of drug distribution in patches was assessed by scanning electron microscopy (S3400 Hitachi, JAPAN) [14][15] . [15][16][17][18] .…”

Section: Morphologymentioning

confidence: 99%

Untitled

2019

IJPSR

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The low bioavailability of drugs in the oral drug delivery system is the main concern and the development of a delivery system with a controlled release profile. This research aims in the dose reduction of aliskiren hemifumarate, evaluate the release efficacy of the drug from the patches by using various permeation enhancers. FTIR studies established the drug and polymer compatibility. The transdermal patches were prepared by using a solvent casting method using different polymers and permeation enhancers. The keshary chein diffusion cell was used for performing in-vitro permeation studies. By physicochemical parameters and in-vitro release pattern, the formulation made from MethocelK 15M (1:1.5) was selected for in-vivo studies. The steady plasma concentration was observed on application of developed transdermal patches, as confirmed by bioavailability studies in rats. The bioavailability was also improved as compared to oral administration. The SEM studies of transdermal patches show that the patches prepared with MethocelK 15M with cineol as penetration enhancer exhibited the uniform distribution of the drug. The hypertension was induced in rats using dexamethasone and treated with oral administration & application of the transdermal patch. The patches significantly controlled hypertension from the first hour. The stability studies indicated satisfactory performance as evaluated for assay & description on storage for six months. Conclusion: The developed transdermal patch exhibited therapeutic superiority over oral drug delivery.

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Sampling Blood from the Lateral Tail Vein of the Rat

Cited by 61 publications

References 12 publications

Local Irradiation Modulates the Pharmacokinetics of Metabolites in 5-Fluorouracil—Radiotherapy–Pharmacokinetics Phenomenon

Local Irradiation Modulates the Pharmacokinetics of Metabolites in 5-Fluorouracil—Radiotherapy–Pharmacokinetics Phenomenon

Hepatoprotective effects ofErythrina abyssinicaLam Ex Dc against Non Alcoholic Fatty Liver Disease in Sprague Dawley Rats

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