Siberian hamsters (Phodopus sungorus) exhibit changes in reproductive and immune function in response to seasonal variations in day length. Exposure to short days induces gonadal regression and inhibits testosterone secretion. In parallel, short days enhance immune function: increasing leukocyte numbers and attenuating cytokine and behavioral responses to infection. We examined whether photoperiodic changes in leukocyte phenotypes and sickness behaviors are dependent on concurrent photoperiodic changes in gonadal function. Male hamsters were gonadectomized or sham-gonadectomized and either exposed to short days (9 h light/day; SD) or kept in their natal long-day (15 h light/day; LD) photoperiod for 10 -13 wk. Blood samples were obtained for leukocyte enumeration, and hamsters were challenged with bacterial LPS, which induced behavioral (anorexia, reductions in nest building) and somatic (weight loss) sickness responses. Among gonad-intact hamsters, exposure to SD increased total and CD62Lϩ lymphocytes and CD3ϩ T lymphocytes in blood and significantly attenuated LPSinduced sickness responses. Independent of photoperiod, castration alone increased total and CD62Lϩ lymphocyte and CD3ϩ T lymphocyte numbers and attenuated somatic and anorexic sickness responses. Among castrated hamsters, SD exposure increased lymphocyte numbers and suppressed sickness behaviors. In castrated hamsters, the magnitude of most immunological effects of SD were diminished relative to those evident in gonad-intact hamsters. The SD phenotype in several measures of immunity can be instated via elimination of gonadal hormones alone; however, photoperiodic effects on immune function persist even in castrated hamsters. Thus, photoperiod affects the immune system and neural-immune interactions underlying sickness behaviors via gonadal hormone-dependent and -independent mechanisms. seasonality; sickness behaviors; infection; neural-immune interactions SEASONAL CHANGES IN PHYSIOLOGY and behavior are legion, most notably in reproductive function (8). Many mammals have evolved mechanisms that permit cessation of reproduction during intervals of the year when environmental conditions of food availability and ambient temperature are unfavorable for successful weaning of offspring (44,47). In nonequatorial regions, changes in day length (photoperiod) predict these environmental constraints (46) and function as proximate cues for triggering anticipatory changes in reproductive physiology and behavior. In long-day breeders, decreasing photoperiods of late-summer trigger gonadal atrophy, withdrawal of gonadal hormone secretion, and cessation of sex behaviors (8). The full complement of changes in the reproductive system can be elicited in the laboratory by exposing reproductively photoperiodic animals to winter-like day lengths (Ͻ12 h of light/day) (22).Seasonal changes in immune function also occur in wild and laboratory animals (34), and a growing body of research indicates photoperiod likewise drives these cycles. Among long-day breeding rodents, many a...