Samsonia erythrinae gen. nov., sp. nov., isolated from bark necrotic lesions of Erythrina sp., and discrimination of plant-pathogenic Enterobacteriaceae by phenotypic features.

Abstract

Bacterial strains isolated from diseased erythrina (Erythrina sp.) trees in Martinique (French West Indies) were studied using phenotypic tests, 16S rDNA sequence analysis and DNA-DNA hybridization. Numerical analysis of phenotypic characteristics showed that these strains formed an homogeneous phenon among plant-pathogenic Enterobacteriaceae, and gave useful and updated information for the identification of these bacteria. Results of DNA-DNA hybridization indicated that strains from erythrina belonged to a discrete genomospecies (89-100 % hybridization) and had low levels of DNA relatedness (2-33 % hybridization) with reference strains of phytopathogenic Erwinia, Brenneria, Pectobacterium, Pantoea and Enterobacter species. 16S rDNA sequence analysis using three different methods revealed that the position of strain CFBP 5236 T isolated from erythrina was variable in the different trees, so that strains from erythrina could not be assigned to any recognized genus. It is proposed that these strains are included in a new genus, Samsonia. The name Samsonia erythrinae is proposed for the new species. The GMC content of the DNA of the type strain, CFBP 5236 T (l ICMP 13937 T ), is 570 mol %.

Characterization of Pantoea dispersa UQ68J: producer of a highly efficient sucrose isomerase for isomaltulose biosynthesis

“…The EMBL and GenBank database accessions used by Hauben et al. (1998) and Sutra et al. (2001) were employed to represent the Enterobacteriaceae family.…”

“…The EMBL and GenBank database accessions used by Hauben et al (1998) and Sutra et al (2001) were employed to represent the Enterobacteriaceae family. Data analyses were conducted using software on WebANGIS-GCG (The Australian Genomic Information Centre, Sydney, Australia).…”

Enterobacteria in the 21st century: a review focused on taxonomic changes

“…The genera Coetzeea and Thorsellia, are the only genera described for the Thorselliaceae family. Even though this review focuses only on genera within the family Enterobacteriaceae described during the 21th century, Table 1 shows all the families and genera that belong to the order Enterobacteriales [13][14][15][16][17][18][19][20][21] , in order to show the genera related to Enterobacteriaceae.…”

Diversity of 16S rDNA sequences of spp. implications for species determinations

“…This analysis using the ML method and several sequences representing some species, rather than a single sequence from the type strain, shows the need for caution in deriving classifications based on this single gene [16][17][18]. Analyses of several genes [19] and consensus analyses of several genes [20] offer more reliable methods for phylogenetic inference for the purpose of classification.…”