Sand and silty-sand soil stabilization using bacterial enzyme–induced calcite precipitation (BEICP)

Hoang, Tung; Alleman, James E.; Çetin, Bora; Ikuma, Kaoru; Choi, Sun-Gyu

doi:10.1139/cgj-2018-0191

Cited by 113 publications

(38 citation statements)

References 58 publications

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“…Results show that the increase of CCC is only one reason for the improvement of UCS of fiber-treated sand, and the level of CCC is not the key factor and sufficient condition to determine the UCS of fiber-reinforced EICP-treated sand [ 28 , 29 , 30 , 31 ]. There was no one-to-one correspondence between UCS and CCC.…”

Section: Discussionmentioning

confidence: 99%

Effect of Incorporating Polyvinyl Alcohol Fiber on the Mechanical Properties of EICP-Treated Sand

et al. 2021

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Enzyme-induced calcium carbonate precipitation (EICP) technology can improve the strength of treated soil. But it also leads to remarkable brittleness of the soil. This study used polyvinyl alcohol (PVA) fiber combined with EICP to solidify sand. Through the unconfined compressive strength (UCS) test, the effect of PVA fiber incorporation on the mechanical properties of EICP-solidified sand was investigated; the distribution of CaCO3 in the sample and the microstructure of fiber-reinforced EICP-treated sand were explored through the calcium carbonate content (CCC) test and microscopic experiment. Compared with the sand treated by EICP, the strength and stiffness of the sand reinforced by the fiber combined with EICP were greatly improved, and the ductility was also improved to a certain extent. However, the increase of CCC was extremely weak, and the inhomogeneity of CaCO3 distribution was enlarged; the influence of fiber length on the UCS and CCC of the treated sand was greater than that of the fiber content. The improvement of EICP-solidified sand by PVA fiber was mainly due to the formation of a “fiber–CaCO3–sand” spatial structure system through fiber bridging, not the increase of CCC.

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Section: Discussionmentioning

confidence: 99%

Effect of Incorporating Polyvinyl Alcohol Fiber on the Mechanical Properties of EICP-Treated Sand

et al. 2021

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“…Applied geomicrobial engineering is an expanding field of studies, including the utilisation of microorganisms to modify the soil chemistry and physical properties for geotechnical purposes [1][2][3][4][5][6][7][8][9][10][11][12]. One promising form of biomineralisation for engineering applications is Microbially Induced Carbonate Precipitation (MICP) [13,14], the MICP process involves the formation and precipitation of CaCO 3 polymorphs (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Microbial metabolic activities associated with MICP include ureolysis, denitrification, ammonification, sulphate reduction, and methane oxidation [4]. Different microorganisms have been found capable of MICP and the most studied urease bacteria are Sporosarcina pasteurii [6,[16][17][18][19][20][21] and Bacillus [22][23][24][25]. A recent review paper compared the performances of those microorganism towards MICP [26].…”

Section: Introductionmentioning

confidence: 99%

“…Limited assessments have been made to test the potential of MICP/biocementation under less favourable conditions such as lower temperatures. Similarly, study of indigenous microorganisms and their potential application for MICP/biocementation are rather limited to certain environments [2,3,6,29,41]. For example, Kang et al (2015) [42] investigated the bioremediation potential of lead from ureolytic bacteria from abandoned metal mines in Korea.…”

Section: Introductionmentioning

confidence: 99%

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Microbially induced calcite precipitation performance of multiple landfill indigenous bacteria compared to a commercially available bacteria in porous media

et al. 2021

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Microbially Induced Carbonate Precipitation (MICP) is currently viewed as one of the potential prominent processes for field applications towards the prevention of soil erosion, healing cracks in bricks, and groundwater contamination. Typically, the bacteria involved in MICP manipulate their environment leading to calcite precipitation with an enzyme such as urease, causing calcite crystals to form on the surface of grains forming cementation bonds between particles that help in reducing soil permeability and increase overall compressive strength. In this paper, the main focus is to study the MICP performance of three indigenous landfill bacteria against a well-known commercially bought MICP bacteria (Bacillus megaterium) using sand columns. In order to check the viability of the method for potential field conditions, the tests were carried out at slightly less favourable environmental conditions, i.e., at temperatures between 15-17°C and without the addition of urease enzymes. Furthermore, the sand was loose without any compaction to imitate real ground conditions. The results showed that the indigenous bacteria yielded similar permeability reduction (4.79 E-05 to 5.65 E-05) and calcium carbonate formation (14.4–14.7%) to the control bacteria (Bacillus megaterium), which had permeability reduction of 4.56 E-5 and CaCO3 of 13.6%. Also, reasonably good unconfined compressive strengths (160–258 kPa) were noted for the indigenous bacteria samples (160 kPa). SEM and XRD showed the variation of biocrystals formation mainly detected as Calcite and Vaterite. Overall, all of the indigenous bacteria performed slightly better than the control bacteria in strength, permeability, and CaCO3 precipitation. In retrospect, this study provides clear evidence that the indigenous bacteria in such environments can provide similar calcite precipitation potential as well-documented bacteria from cell culture banks. Hence, the idea of MICP field application through biostimulation of indigenous bacteria rather than bioaugmentation can become a reality in the near future.

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“…Wei and Zhang (2018) reviewed and prospected the improvement of soils from the separation of halophilic fungi, gene of salt and alkaline resistant fungi, macromolecular degrading enzyme, and application of halophilic fungi in the repair of fungi in saline alkali soil [26]. Hoang et al (2019) extracted a new microbial catalyst from sand and muddy soil, which can replace the traditional microbial enzyme to induce calcite precipitation, making new improvements in biosafety and geotechnical engineering [27]. In the past, most of the research focuses on simply discussing the change rule of the mechanical properties of soil by solidifying agent.…”

Section: Introductionmentioning

confidence: 99%

Effect of Freeze-Thaw Cycle on Shear Strength of Lime-Solidified Dispersion Soils

Meng

Wang

et al. 2020

Civ Eng J

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The freeze-thaw cycle of saline soil in the seasonal frozen area will produce diseases such as frost heave and thaw settlement, road frost boiling, collapse and uneven settlement. In order to reduce the occurrence of these undesirable phenomena, it is often necessary to improve the saline soil in engineering. In this paper, the typical carbonate saline soil in the west of Jilin Province, China is taken as the research object. By adding different content of lime (0%, 3%, 6%, 9%, 12%, 15%), the change of mechanical strength of lime solidified saline soil under different freeze-thaw cycles (0, 1, 3, 6, 10, 30, 60 times) is studied. The mechanical analysis is carried out by combining particle size analysis test and SEM image. The test results show that although repeated freeze-thaw cycles make the soil structure loose and the mechanical strength greatly reduced, the soil particles agglomerate obviously after adding lime, its dispersion is restrained by the flocculation of clay colloid, and the shear strength of soil is improved by the increase of the cohesive force between clay particles, and the optimal lime mixing ratio of the saline soil in this area is 9%.

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Sand and silty-sand soil stabilization using bacterial enzyme–induced calcite precipitation (BEICP)

Cited by 113 publications

References 58 publications

Effect of Incorporating Polyvinyl Alcohol Fiber on the Mechanical Properties of EICP-Treated Sand

Effect of Incorporating Polyvinyl Alcohol Fiber on the Mechanical Properties of EICP-Treated Sand

Microbially induced calcite precipitation performance of multiple landfill indigenous bacteria compared to a commercially available bacteria in porous media

Effect of Freeze-Thaw Cycle on Shear Strength of Lime-Solidified Dispersion Soils

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