An experiment was conducted to observe the effect of different diets containing different level of non-starch polysaccharides (NSP) on performance and cannibalism of laying hens. Six diets (wheatbased, rice hull-based, plant protein-based, millrun-based, MOS, and bentonite) were used and were randomly given to 6 groups of ISA Brown hens, with 18 replicates per group and 5 birds per replicate for 8 weeks. The results showed that diets did not have a significant effect (P>0.05) on cannibalism mortality, but numerically the rice hull diet gave the lowest effect, the millrun gave the intermediate effect, whereas the plant protein diet gave the largest negative effect. Diet significantly affected feed intake (P<0.01), egg production (P<0.01), feed to egg ratio (P<0.01) and egg weight (P<0.05). Birds on millrun diet had the lowest intake, but the feed to egg ratio was superior and egg production was the highest compared to those fed other diets. In contrast, the feed to egg ratio in rice hull diet was inferior, and egg weight was also lighter than those fed other diets. In conclusion, fibre sources have been verified as contributing factors involved in the outbreak of cannibalism in laying hens. Diets containing high insoluble NSP had a potential to decrease the mortality due to cannibalism.