Sandflies (Diptera: Psychodidae) from caves of the quartzite Espinhaço Range, Minas Gerais, Brazil

Barata, Ricardo Andrade; Apolinário, Estefânia Conceição

doi:10.1590/s0074-02762012000800009

Cited by 9 publications

(12 citation statements)

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“…The potential touristic use of caves is very high and this activity can cause a decrease in sandfly diversity and an increase in human contact with leishmaniasis vectors (ALVES et al, 2011;BARATA & APOLINÁRIO, 2012). In this study, we found eight species that have already been correlated with leishmaniasis transmission: Bichromomyia reducta was found naturally infected with Leishmania (Leishmania) amazonensis Lainson & Shaw in state of Rondônia (FREITAS et al, 1989).…”

Section: Discussionmentioning

confidence: 65%

“…In Minas Gerais, Barata & Apolinário (2012) collected 17 species, and Carvalho et al (2013) collected 29 species, thus demonstrating that caves are rich environments for these insects. The majority of the species collected in Rondônia have already been found in other studies, and they are typical in the Amazon environment (ALVES et al, 2011;PEREIRA et al, 2015;SILVA et al, 2014).…”

Section: Discussionmentioning

confidence: 97%

“…This might also affect the number of individuals collected (ALVES et al, 2011;CARVALHO et al, 2013). Species have different preferences, but collections made right after the beginning of the rainy season usually result in higher numbers of specimens than in collections made at the end of the rainy season and in the dry season (BARATA & APOLINÁRIO, 2012). Nonetheless, there have been reports of high abundance of these insects at the end of the rainy season (GALARDO et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Over recent years, sandflies have been found in other environments with human intervention such as fragmented forests and peridomestic areas, thus showing high adaptive capacity (PESSOA et al, 2007;PEREIRA et al, 2015). Caves are environments in which sandflies have been frequently found (ALVES et al, 2011;BARATA & APOLINÁRIO, 2012;CARVALHO et al, 2013). Inside caves, some factors are favorable to the sandflies' life cycle, like humidity, temperature, organic matter and little or no presence of light (ALENCAR et al, 2011;WILLIAMS, 1976).…”

Section: Introductionmentioning

confidence: 99%

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Sandfly fauna (Diptera: Psychodidae) from caves in the state of Rondônia, Brazil

Ogawa

Júnior

Resadore

et al. 2016

Rev. Bras. Parasitol. Vet.

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This study had the aim of ascertaining the sandfly fauna and possible presence of Leishmania in these insects, collected in caves in the state of Rondônia, Brazil. Collections were conducted in eight caves located in two different areas of this state. Leishmania in the sandflies collected was detected using the polymerase chain reaction (PCR). This was the first study on sandflies from caves in Rondônia and, among the total of 1,236 individuals collected, 24 species and 10 genera were identified. The species Evandromyia georgii was collected for the first time in Rondônia and the most abundant species were Trichophoromyia ubiquitalis with 448 individuals (36.2%), followed by T. octavioi with 283 (22.9%) and E. georgii with 179 (14.5%). For the PCR, 17 pools were analyzed and five pools were positive (for T. auraensis in three pools and for Nyssomyia shawi and N. antunesi in one pool each). The kDNA region was amplified and the presence of Leishmania DNA was confirmed. The sandfly fauna in these caves can be considered diverse in comparison with similar studies in other regions. It may be that some species use caves as a temporary shelter and breeding site, while other species live exclusively in this environment. The detection of Leishmania DNA indicates that this pathogen is circulating in cave environments and that further studies are needed in order to ascertain the risks of infection by leishmaniasis in these locations with high touristic potential.Keywords: Caves, sandflies, Rondônia, Amazon. ResumoO objetivo deste estudo foi conhecer a fauna de flebotomíneos, e possível presença de Leishmania nestes insetos, coletados em cavernas do Estado de Rondônia. As coletas foram realizadas em oito cavernas localizadas em duas áreas diferentes do Estado. A detecção de Leishmania nos flebotomíneos foi realizada por reação em cadeia da polimerase (PCR). Este foi o primeiro trabalho com flebotomíneos em cavernas de Rondônia e um total de 1,236 indivíduos foram coletados e identificados em 24 espécies e 10 gêneros. Evandromyia georgii foi coletada pela primeira vez em Rondônia, e as espécies mais abundantes foram Trichophoromyia ubiquitalis com 448 indivíduos (36.2%) seguida por T. octavioi com 283 (22.9%) e E. georgii com 179 (14.5%). No estudo de PCR, 17 pools foram analisados, sendo cinco positivos (T. auraensis -3, Nyssomyia shawi e N. antunesi -1 cada). A região do kDNA foi amplificada confirmando a presença de DNA de Leishmania. A fauna de flebotomíneos nestas cavernas foi considerada diversa quando comparada com estudos semelhantes de outras regiões. É possível que algumas espécies utilizem cavernas como abrigo temporário e local de procriação e outras sejam exclusivas deste ambiente. A detecção de DNA de Leishmania indica que este patógeno está circulando no ambiente cavernícola, sendo necessários mais estudos para conhecer o risco de transmissão de leishmanioses nestes locais com alto potencial turístico.Palavras-chave: Cavernas, flebotomíneos, Rondônia, Amazônia.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sandfly fauna (Diptera: Psychodidae) from caves in the state of Rondônia, Brazil

Ogawa

Júnior

Resadore

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…Although some important studies have been establishing the effects of seasonal variations on L. longipalpis distribution, the consequences of seasonality on daily activity [11-15] was not explored in detail.…”

Section: Discussionmentioning

confidence: 99%

Effects of temperature and photoperiod on daily activity rhythms of Lutzomyia longipalpis (Diptera: Psychodidae)

et al. 2014

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BackgroundInsect vectors have been established as models in Chronobiology for many decades, and recent studies have demonstrated a close relationship between the circadian clock machinery, daily rhythms of activity and vectorial capacity. Lutzomyia longipalpis, the primary vector of Leishmania (Leishmania) infantum in the New World, is reported to have crepuscular/nocturnal activity in the wild. However, most of these studies applied hourly CDC trap captures, which is a good indicative of L. longipalpis behaviour, but has limited accuracy due to the inability to record the daily activity of a single insect during consecutive days. In addition, very little is known about the activity pattern of L. longipalpis under seasonal variations of average temperature and day length in controlled laboratory conditions.MethodsWe recorded the locomotor activity of L. longipalpis males under different artificial regimes of temperature and photoperiod. First, in order to test the effects of temperature on the activity, sandflies were submitted to regimes of light/dark cycles similar to the equinox photoperiod (LD 12:12) combined with different constant temperatures (20°C, 25°C and 30°C). In addition, we recorded sandfly locomotor activity under a mild constant temperature (25°C with different day length regimes: 8 hours, 12 hours and 16 hours).ResultsL. longipalpis exhibited more activity at night, initiating dusk-related activity (onset time) at higher rather than lower temperatures. In parallel, changes of photoperiod affected anticipation as well as all the patterns of activity (onset, peak and offset time). However, under LD 16:08, sandflies presented the earliest values of maximum peak and offset times, contrary to other regimes.ConclusionsHerein, we showed that light and temperature modulate L. longipalpis behaviour under controlled laboratory conditions, suggesting that sandflies might use environmental information to sustain their crepuscular/nocturnal activity, as well as other important aspects as mating and host-seeking at appropriate times in different seasons. Our results depict previously unappreciated aspects of the L. longipalpis daily rhythms of activity that might have important epidemiological implications.

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First molecular evidence of frogs as a food source for sand flies (Diptera: Phlebotominae) in Brazilian caves

et al. 2021

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Sandflies (Diptera: Psychodidae) from caves of the quartzite Espinhaço Range, Minas Gerais, Brazil

Cited by 9 publications

References 20 publications

Sandfly fauna (Diptera: Psychodidae) from caves in the state of Rondônia, Brazil

Sandfly fauna (Diptera: Psychodidae) from caves in the state of Rondônia, Brazil

Effects of temperature and photoperiod on daily activity rhythms of Lutzomyia longipalpis (Diptera: Psychodidae)

First molecular evidence of frogs as a food source for sand flies (Diptera: Phlebotominae) in Brazilian caves

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