Sandwich ELISA for detection of horse meat in raw meat mixtures using antisera to muscle soluble proteins

Martı́n, Rosario; Azcona, Juan I.; Garcı́a, Teresa; Hernández, Pablo E.; Sanz, B.

doi:10.1016/0309-1740(88)90088-5

Cited by 19 publications

(8 citation statements)

References 23 publications

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“…The resulting spectroscopic data was pre-processed and subjected to PCA. The results are shown in Figure 3A DNA targets associated with heat treatment, [19][20][21] however a number of ELISA-based and LCbased methods provide excellent results for heat-treated samples. [22][23][24] In this aspect, the REIMSbased identification methods are also largely insensitive to heat treatment.…”

Section: Resultsmentioning

confidence: 99%

“…The preparation of sample has clearly no effect on the differentiation of the species. Most well-established tests available for the identification of horse meat in food items employ either immunochemical or molecular genetic methods, and both of these methods suffer from the hydrolysis of protein or DNA targets associated with heat treatment; − however, a number of ELISA-based and LC-based methods provide excellent results for heat-treated samples. − In this aspect, the REIMS-based identification methods are also largely insensitive to heat treatment.…”

Section: Resultsmentioning

confidence: 99%

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Identification of the Species of Origin for Meat Products by Rapid Evaporative Ionization Mass Spectrometry

Balog

Perenyi

Guallar-Hoyas

et al. 2016

J. Agric. Food Chem.

128

100

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ABSTRACT. The increasingly abundant food fraud cases have brought food authenticity and 1 safety into major focus. In this study, we present a fast and effective way to identify meat 2 products using rapid evaporative ionization mass spectrometry (REIMS). The experimental setup 3 was demonstrated to be able to record a mass spectrometric profile of meat specimens in a time 4 frame of less than 5 seconds. A multivariate statistical algorithm was developed and successfully 5 tested for the identification of animal tissue with different anatomical origin, breed and species 6 with 100% accuracy at species and 97% accuracy at breed level. Detection of the presence of 7 meat originating from a different species (horse, cattle, and venison) has also been demonstrated 8 with high accuracy using mixed patties with a 5% detection limit. REIMS technology was found 9to be a promising tool in food safety applications providing a reliable and simple method for the 10 rapid characterization of food products. 11 12

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Identification of the Species of Origin for Meat Products by Rapid Evaporative Ionization Mass Spectrometry

Balog

Perenyi

Guallar-Hoyas

et al. 2016

J. Agric. Food Chem.

128

100

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“…However, as muscle proteins are highly conserved between species, we considered it important to use partially purified specific antigens for the immunisation of mice. To obtain the horse-specific antigens, previously obtained horse-specific polyclonal antibodies (Martin et al 1988a) were immobilised on a protein A-Sepharose CL4B column and used for isolation of protein fractions richer in horse-specific epitopes (Martin et al 1992).…”

Section: Discussionmentioning

confidence: 99%

“…able for raw meat speciation use polyclonal antibodies raised against blood proteins (Griffiths and Billington 1984; Patterson et a1 1984; Patterson 1985, 1986; Cutrufelli et al 1986; Allsup 1987; Kang'ethe et a1 1987) or soluble muscle proteins (Martin et al 1986(Martin et al , 1988a. Although immunological methods are accurate and sufficiently sensitive for the identification of * To whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

Production of a horse‐specific monoclonal antibody and detection of horse meat in raw meat mixtures by an indirect ELISA

et al. 1994

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A stable hybridoma cell line (DD3) has been produced secreting a monoclonal antibody specific for horse muscle proteins. The DD3 monoclonal antibody (mAb) did not show significant cross-reactivity when tested against beef, chicken, pig and soya proteins or bovine caseins, gelatine and bovine serum albumin. The DD3 mAb was used in an indirect ELISA format for the detection of defined amounts of horse meat (10-500 g kg-') in beef meat mixtures. Immunorecognition of monoclonal antibodies adsorbed to horse meat adsorbed onto the ELISA plate was made with rabbit anti-mouse immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymic conversion of the substrate gave clear optical density differences when assaying mixtures of minced beef containing different amounts of horse meat.

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“…A sandwich enzyme-linked immunosorbent assay (sELISA) using antisera has also been developed to detect horse meat in raw meat mixtures. 16 However, this method is applicable only to raw meat samples and the use of antisera or polyclonal antibodies (pAbs), which vary in quality from batch to batch, makes it unattractive as a reliable method for monitoring horse meat adulteration. The 96-well microplate format test kit marketed by ELISA Technologies Inc. (Gainesville FL, USA), suffers from similar limitations as it is also based on pAbs.…”

Section: ■ Introductionmentioning

confidence: 99%

Detection of Horse Meat Contamination in Raw and Heat-Processed Meat Products

Hsieh

Ofori

2014

J. Agric. Food Chem.

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Europe's recent problems with the adulteration of beef products with horse meat highlight the need for a reliable method for detecting horse meat in food for human consumption. The objective of this study was therefore to develop a reliable monoclonal antibody (mAb) based enzyme-linked immunosorbent assay (ELISA) for horse meat detection. Two mAbs, H3E3 (IgG2b) and H4E7 (IgG2a), were characterized as horse-selective, and competitive ELISAs (cELISAs) employing these mAbs were developed. The cELISAs were found to be capable of detecting levels as low as 1% of horse meat in raw, cooked, and autoclaved ground beef or pork, being useful analytical tools for addressing the health, economic, and ethical concerns associated with adulterating meat products with horse meat. However, due to cross-reaction with raw poultry meat, it is recommended that samples be heated (100 °C for 15 min) prior to analysis to eliminate possible false-positive results.

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Sandwich ELISA for detection of horse meat in raw meat mixtures using antisera to muscle soluble proteins

Cited by 19 publications

References 23 publications

Identification of the Species of Origin for Meat Products by Rapid Evaporative Ionization Mass Spectrometry

Identification of the Species of Origin for Meat Products by Rapid Evaporative Ionization Mass Spectrometry

Production of a horse‐specific monoclonal antibody and detection of horse meat in raw meat mixtures by an indirect ELISA

Detection of Horse Meat Contamination in Raw and Heat-Processed Meat Products

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