Sandwich enzyme-linked immunosorbent assay for naringin

Qu, Huihua; Wang, Xueqian; Qu, Baoping; Kong, Hui; Zhang, Yue; Shan, Wenchao; Cheng, Jinjun; Wang, Qingguo; Zhao, Yan

doi:10.1016/j.aca.2015.09.058

Cited by 16 publications

(9 citation statements)

References 16 publications

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“…However, it is difficult to prepare two antibodies possessing different epitopes, especially for haptens because steric hindrance may disturb the antigen–antibody reaction because of the small size of the haptens. Therefore, true sandwich assays for hapten can be rarely developed, except for tacrolimus [ 53 ], angiotensin II [ 54 ], and naringin (Nar; which is a major flavonoid glycoside found in citrus fruits) [ 55 ]. Among these haptens, Nar is the smallest hapten with a molecular weight of 580 Da and the only plant secondary metabolite.…”

Section: Elisa For Plant Secondary Metabolitesmentioning

confidence: 99%

“…Among these haptens, Nar is the smallest hapten with a molecular weight of 580 Da and the only plant secondary metabolite. Two hybridoma cell lines secreting different antibodies have been carefully screened, which enabled construction of a sandwich for Nar [ 55 ]. Interestingly, specificity to Nar in sandwich ELISA using two MAbs dramatically increases as compared with that in icELISA using a single MAb.…”

Section: Elisa For Plant Secondary Metabolitesmentioning

confidence: 99%

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Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites

et al. 2017

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Immunoassays are antibody-based analytical methods for quantitative/qualitative analysis. Since the principle of immunoassays is based on specific antigen–antibody reaction, the assays have been utilized worldwide for diagnosis, pharmacokinetic studies by drug monitoring, and the quality control of commercially available products. Berson and Yalow were the first to develop an immunoassay, known as radioimmunoassay (RIA), for detecting endogenous plasma insulin [1], a development for which Yalow was awarded the Nobel Prize in Physiology or Medicine in 1977. Even today, after half a century, immunoassays are widely utilized with some modifications from the originally proposed system, e.g., radioisotopes have been replaced with enzymes because of safety concerns regarding the use of radioactivity, which is referred to as enzyme immunoassay/enzyme-linked immunosorbent assay (ELISA). In addition, progress has been made in ELISA with the recent advances in recombinant DNA technology, leading to increase in the range of antibodies, probes, and even systems. This review article describes ELISA and its applications for the detection of plant secondary metabolites.

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Section: Elisa For Plant Secondary Metabolitesmentioning

confidence: 99%

Section: Elisa For Plant Secondary Metabolitesmentioning

confidence: 99%

Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites

et al. 2017

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“…Then, unknown or known samples are added into the matrix to minimize attachment to the solid phase. Peroxidase-enzyme labeled antibody is then added for coloration, which is directly proportional to the amount of antigen present (Qu et al, 2016).…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

Antibody Engineering for Pursuing a Healthier Future

et al. 2017

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Since the development of antibody-production techniques, a number of immunoglobulins have been developed on a large scale using conventional methods. Hybridoma technology opened a new horizon in the production of antibodies against target antigens of infectious pathogens, malignant diseases including autoimmune disorders, and numerous potent toxins. However, these clinical humanized or chimeric murine antibodies have several limitations and complexities. Therefore, to overcome these difficulties, recent advances in genetic engineering techniques and phage display technique have allowed the production of highly specific recombinant antibodies. These engineered antibodies have been constructed in the hunt for novel therapeutic drugs equipped with enhanced immunoprotective abilities, such as engaging immune effector functions, effective development of fusion proteins, efficient tumor and tissue penetration, and high-affinity antibodies directed against conserved targets. Advanced antibody engineering techniques have extensive applications in the fields of immunology, biotechnology, diagnostics, and therapeutic medicines. However, there is limited knowledge regarding dynamic antibody development approaches. Therefore, this review extends beyond our understanding of conventional polyclonal and monoclonal antibodies. Furthermore, recent advances in antibody engineering techniques together with antibody fragments, display technologies, immunomodulation, and broad applications of antibodies are discussed to enhance innovative antibody production in pursuit of a healthier future for humans.

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“…Our team successfully developed, for the first time, a sandwich ELISA for Nar ( Figure 2 ) providing an improved analytical approach with a broader detection range and higher precision compared to the icELISA utilizing each anti-Nar MAbs separately [ 89 ]. It is considered to be the only sandwich ELISA established for MPNP.…”

Section: Immunoassay For Mpnp Using Mabsmentioning

confidence: 99%

“… A true sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for naringin and the comparison with ELISA [ 89 ]. Reprinted with permission from Huihua Qu et al (2015), Copyright 2015 Analytica Chimica Acta.…”

Section: Figurementioning

confidence: 99%

Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review

et al. 2017

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Owing to the widespread application value, monoclonal antibodies (MAbs) have become a tool of increasing importance in modern bioscience research since their emergence. Recently, some researchers have focused on the production of MAbs against medical plant-derived natural products (MPNP), the secondary metabolites of medical plants. At the same time, various immunoassay methods were established on the basis of these MPNP MAbs, and then rapidly developed into a novel technique for medical plant and phytomedicine research in the area of quality control, pharmacological analysis, drug discovery, and so on. Dependent on the research works carried out in recent years, this paper aims to provide a comprehensive review of MAbs against MPNP and the application of various immunoassay methods established on the basis of these MAbs, and conclude with a short section on future prospects and research trends in this area.

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Sandwich enzyme-linked immunosorbent assay for naringin

Cited by 16 publications

References 16 publications

Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites

Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites

Antibody Engineering for Pursuing a Healthier Future

Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review

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