Sandwich Fluorescence Detection of Foodborne Pathogen Staphylococcus aureus with CD Fluorescence Signal Amplification in Food Samples

Du, Han; Ping, Tao; Wu, Wei; Yang, Qingli

doi:10.3390/foods11070945

Cited by 14 publications

(6 citation statements)

References 47 publications

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“…An aptamer (Apt) is a highly specific and selective oligonucleotide sequence with high affinity and specificity for specific screening targets [87]. Du [88] prepared composite materials using CQDs and DNA tetrahedral signal amplifiers for the detection of Staphylococcus aureus and Salmonella typhimurium. Suitable sequences were screened for the synthesis of DNA tetrahedra as well as aptamers using the NUPACK nucleic acid analysis design software.…”

Section: Nucleic Acid Aptamer Specific Recognitionmentioning

confidence: 99%

Advances in carbon quantum dot technology for food safety: preparation, modification, applications and mechanisms

Sun,

Cheng,

et al. 2024

Nano Ex.

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Functionalized carbon quantum dots (CQDs) show great potential for application in the field of food safety by combining their use with other technologies. CQDs are good candidates in this regard due to the wide range of sources of raw materials for their synthesis, and their good biocompatibility and stable fluorescence. This paper analyses the properties of CQDs and compares them with those of conventional semiconductor quantum dots. It analyses the similarities and differences between hydrothermal carbonization, pyrolysis and microwave-assisted synthesis of CQDs, and reviews the principles and methods of functionalization of CQDs through surface modification and doping. Finally, it discusses the applications of functionalized CQDs in food safety, such as detection and sensing, bio-inhibition and photocatalytic degradation, and the mechanisms of detection.

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Section: Nucleic Acid Aptamer Specific Recognitionmentioning

confidence: 99%

Advances in carbon quantum dot technology for food safety: preparation, modification, applications and mechanisms

Sun,

Cheng,

et al. 2024

Nano Ex.

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show abstract

“…In other promising studies, the sensitive biosensors have been developed for detecting S. aureus by Yang et al (2021) and Ouyang et al (2022). Zhang et al (2021) and Du et al (2022) have developed colorimetric and fluorescent-based aptasensor for the detection of this foodborne pathogen.…”

Section: Aptamermentioning

confidence: 99%

“…(2021) and Du et al. (2022) have developed colorimetric and fluorescent‐based aptasensor for the detection of this foodborne pathogen.…”

Section: Biorecognition Elementsmentioning

confidence: 99%

“…This biosensor can detect that foodborne pathogen as low as 43 cfu mL −1 . Blue carbon quantum dots (bCDs) were used as fluorescence signal amplifier for the detection of S. aureus (Du et al., 2022). The target bacteria were sandwiched between DNA tetrahedron (Td)‐aptamer structure modified with magnetic beads and bCDs.…”

Section: Incorporation Of Nanomaterials Into Biosensor For Signal Amp...mentioning

confidence: 99%

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Nanomaterial interfaces designed with different biorecognition elements for biosensing of key foodborne pathogens

Atay

Altan

2023

Comp Rev Food Sci Food Safe

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Foodborne diseases caused by pathogen bacteria are a serious problem toward the safety of human life in a worldwide. Conventional methods for pathogen bacteria detection have several handicaps, including trained personnel requirement, low sensitivity, laborious enrichment steps, low selectivity, and long‐term experiments. There is a need for precise and rapid identification and detection of foodborne pathogens. Biosensors are a remarkable alternative for the detection of foodborne bacteria compared to conventional methods. In recent years, there are different strategies for the designing of specific and sensitive biosensors. Researchers activated to develop enhanced biosensors with different transducer and recognition elements. Thus, the aim of this study was to provide a topical and detailed review on aptamer, nanofiber, and metal organic framework–based biosensors for the detection of food pathogens. First, the conventional methods, type of biosensors, common transducer, and recognition element were systematically explained. Then, novel signal amplification materials and nanomaterials were introduced. Last, current shortcomings were emphasized, and future alternatives were discussed.

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“…Certain rapid methods, such as nearinfrared spectroscopy and spectrophotometry, still involve tedious operations and poor repeatability. Recently, fluorescence sensing methods have been widely used for food assessment, owing to their high operational simplicity, high sensitivity, and short response time [10,11]; however, these fluorescence methods need to be improved, owing to the requirement of specialized instruments [12]. Eye-readable sensors have attracted increasing attention for their simple and convenient assessment, without the need for electronics and external digital readouts [13,14].…”

Section: Introductionmentioning

confidence: 99%

Easy-to-Use Visual Sensing System for Milk Freshness, Sensitized with Acidity-Responsive N-Doped Carbon Quantum Dots

Zhang

et al. 2022

Foods

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This study established a flexible and eye-readable sensing system for the easy-to-use, visual detection of milk freshness, using acidity-responsive N-doped carbon quantum dots (N-CQDs). N-CQDs, rich in amino groups and with characteristic acidity sensitivity, exhibited high relative quantum yields of 25.2% and an optimal emission wavelength of 567 nm. The N-CQDs fluorescence quenching upon the dissociated hydrogen ions (H+) in milk and their reacting with the amino groups produced an excellent linear relation (R2 = 0.996) between the fluorescence intensity and the milk acidity, which indicated that the fluorescence of the N-CQDs was highly correlated with milk freshness. Furthermore, a fluorescence sensor was designed by depositing the N-CQDs on filter-papers and starch-gel films, to provide eye-readable signals under UV light. A fluorescence colorimetric card was developed, based on the decrease in fluorescence brightness as freshness deteriorated. With the advantages of high sensitivity and eye readability, the proposed sensor could detect spoiled milk in advance and without any preprocessing steps, offering a promising method of assessing food safety.

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Sandwich Fluorescence Detection of Foodborne Pathogen Staphylococcus aureus with CD Fluorescence Signal Amplification in Food Samples

Cited by 14 publications

References 47 publications

Advances in carbon quantum dot technology for food safety: preparation, modification, applications and mechanisms

Advances in carbon quantum dot technology for food safety: preparation, modification, applications and mechanisms

Nanomaterial interfaces designed with different biorecognition elements for biosensing of key foodborne pathogens

Easy-to-Use Visual Sensing System for Milk Freshness, Sensitized with Acidity-Responsive N-Doped Carbon Quantum Dots

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