Sandwich fluorometric method for dual-role recognition of Listeria monocytogenes based on antibiotic-affinity strategy and fluorescence quenching effect

Upconversion Fluorescence Nanoprobe-Based FRET for the Sensitive Determination of Shigella

“…To get over these issues, some new fluorescence probes based on lanthanide-doped upconversion nanoparticles (UCNPs) fluorescence materials were fabricated to detect foodborne pathogens. Diametrically opposed to the traditional fluorescence materials, UCNPs have the following advantages [ 16 , 17 , 18 , 19 , 20 , 21 ]: (1) easy functionalization by some biologically active substances; (2) multiple fluorescence emission peaks can be obtained by doping with different rare earth elements and a high anti-Stoke shift; (3) innocuity, high resistance to photobleaching, long fluorescence lifetime and low auto-fluorescence background; and (4) ability to convert low-energy photons (such as 980 nm excitation light) into high-energy photons (such as yellow-green fluorescence). The above advantages make the fluorescence probe constructed by UCNPs display high sensitivity, simple operation, and high accuracy in food safety detection.…”

“…In addition, some novel upconversion fluorescent probes have been developed for the detection of foodborne pathogens in food. For instance, the UCNPs−WS2 fluorescence probe based on the fluorescence resonance energy transfer (FRET) was fabricated to detect Escherichia coli ( E. coli ) in water and tea powder samples with high sensitivity (LOD =17 CFU/mL) and high accuracy (96.5–101.6%) [ 28 ]; a sandwich upconversion fluorescence probe based on an inner filter (IFE) for Listeria monocytogenes ( L. monocytogenes ) in ham samples was reported with a low detection limit (2.8 × 10 2 CFU/mL) and high selectivity [ 16 ]; a UCNPs−MTX PCR fluorescence probe based on IFE was developed to detect Salmonella typhimurium ( S. typhimurium ) DNA in chicken samples with high sensitivity (11 CFU/mL) and high selectivity. The above upconversion fluorescent probes have shown excellent performance in the detection of pathogenic bacteria in food.…”

On‐site rapid detection of Listeria monocytogenes in dairy products using smartphone‐integrated device‐assisted ratiometric fluorescent sensors

“…In addition, the DF value was inversely proportional to the concentration of L. monocytogenes in the linear range from 3.9 9 10 1 to 3.9 9 10 7 cfu/mL. In contrast, most studies of detecting L. monocytogenes focused on rapid modes of fluorescence-colorimetric strategy, isothermal sequence exchange amplification or fluorescence quenching method, which led to restriction in complex food without better signal correction, limit of unmanageable amplification efficiency or confine of unstable colour during measurement, relatively (Shen et al 2022;Li et al 2022a;2022b). The design of our ratiometric fluorescent was successful for detecting L. monocytogenes with built-in self-calibration, resulting in good linear range and detection limit, which compared to Besides, as shown in Figure 5, the selectivity of the ratiometric fluorescent system was employed to test practical potential for complex dairy samples.…”

“…Currently, colorimetric or fluorescence strategies of optical sensing biosensors have been conducted for the detection of food allergens, toxic pesticides, harmful metal ions, bacterial biotoxin and pathogenic bacteria (Tang et al 2023). These detection strategies required enrichment procedures or separation steps from complex food matrices, like high-protein milk, resulting in long time-consuming, and low accuracy (Feng et al 2022;Li et al 2022a). In light of these limitations, the need is to consider the interference posed by proteins, lipids and chromogens in food substrates, as well as the desire for more suitable on-site testing.…”

“…2022; Li et al . 2022a). In light of these limitations, the need is to consider the interference posed by proteins, lipids and chromogens in food substrates, as well as the desire for more suitable on‐site testing.…”