Sandwich-type immunosensors and immunoassays exploiting nanostructure labels: A review

Pei, Xiaomei; Zhang, Bing; Tang, Juan; Liu, Bingqian; Lai, Wenqiang; Tang, Dianping

doi:10.1016/j.aca.2012.10.060

Cited by 426 publications

(206 citation statements)

References 251 publications

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“…The stock of I + J library and helper phage KM 13 was expanded to retain sufficient quantity in several rounds of the panning procedure. The library was amplied, and the phage particles were rescued by superinfection with the helper phage.…”

Section: Panning and Selection Of Morphine Scfv Clonesmentioning

confidence: 99%

“…[10][11][12] These methods were based on sandwich immunoassay for the detection of multivalent antigen. 13 In recent times, photoelectrochemical immunoassays have attracted signicant attention due to their low cost and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

“…This library contained (in phagemid/scFv format -fused to the pIII minor coat protein of M 13 bacteriophage) helper phage KM 13 and E. coli strains TG1 and HB2151 for the selection of specic antibody clones and production of soluble single-chain Fvs, respectively. The scFv phagemid library contained synthetic V-gene (V H -V L ) from lox library vector recloned into a (pHEN2) phagemid vector.…”

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confidence: 99%

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A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

et al. 2018

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Gold nanoparticle (AuNP)-based optical assays are of significant interest since the molecular phenomenon can be examined easily with change in the color of AuNPs. Herein, we report the development of a dipstick using a AuNP-labeled single-chain fragment variable (scFv) antibody for the detection of morphine. The scFv antibodies for morphine were developed using phage display-based antibody library. Immunoglobulin variable regions of heavy (V H )-and light (V L )-chain genes were connected via a glycine-serine linker isolated from murine immune repertoire and cloned into the expression vector pIT2. The scFv was produced in Escherichia coli HB2151, yielding a functional protein with a molecular weight of approximately 32 kDa. The morphine scFv was labeled with gold nanoparticles and used as an optical immunoprobe in a dipstick. The competitive dipstick assay characterized the ability of the scFv antibody to recognize free morphine. The detection range was 1-1000 ng mL À1 with a limit of detection (LOD) of 5 ng mL À1 under optimal conditions, and the IC 50 value was 14 ng mL À1 for morphine. The developed optical dipstick kit of scFv antibody was capable of specifically binding to free morphine and its analogs in a solution in less than 5 min and could be useful for on-site screening of a real sample in blood, urine, and saliva.

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Section: Panning and Selection Of Morphine Scfv Clonesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

et al. 2018

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“…Thus, the first consideration for amplifying the detection signal is to use more label molecules for one immunological recognition event. In recent years, great efforts have been made to amplify the signal output by increasing the ratio of signal tag to specific recognition ligand using nanomaterials as carriers [5,6] or signal tags, or molecular biological technology [7,8] to introduce more reporter molecules. Due to the high surface-to-volume ratio of nanomaterials, it is an efficient method to use them as the carriers labeling numerous reporter molecules by immobilizing enzymes or nanoparticle on their surface.…”

Section: Nanomaterials As Carriers For Multi-labeling Immunosensingmentioning

confidence: 99%

Signal Amplification for Highly Sensitive Immunosensing

2017

J. Anal. Test.

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To dissolve the bottleneck problem of life and biomedical science in detection of biomolecules with low abundance and acquisition of ultraweak biological signals, highly sensitive analytical methods coupling with the specificity of biological recognition events have been quickly developed by the exploring of signal amplification strategies. These strategies have extensively been introduced into the development of highly sensitive immunosensing methods by combining with highly specific immunological recognition. They can be divided into two groups. One group of strategies attempts to transfer the immunological recognition event into large number of reporter probes or signal probes for signal readout by employing nano/micro-materials as vehicles for multi-labeling and/or molecular biological amplification for increasing the abundance of the signal molecules. The other uses nanomaterials or enzyme mimics as catalytic tools/tags to obtain enhanced detection signal. This review focuses on the significant advances in design of signal amplification strategies for highly sensitive immunosensing.

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“…4 In the electrochemical immunoassay, the on-line amplification of a detectable signal is extremely important to acquire high sensitivity. [5][6][7] Although the antigen-antibody reaction by itself can cause a change of the electrochemical signal to some extent, it is very minor. Enzyme labels are usually used for the amplification of a detectable signal.…”

Section: Introductionmentioning

confidence: 99%

Guanine-Decorated Graphene Nanostructures for Sensitive Monitoring of Neuron-Specific Enolase Based on an Enzyme-Free Electrocatalytic Reaction

Tian

2013

ANAL. SCI.

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Sandwich-type immunosensors and immunoassays exploiting nanostructure labels: A review

Cited by 426 publications

References 251 publications

A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

A gold nanoparticle-single-chain fragment variable antibody as an immunoprobe for rapid detection of morphine by dipstick

Signal Amplification for Highly Sensitive Immunosensing

Guanine-Decorated Graphene Nanostructures for Sensitive Monitoring of Neuron-Specific Enolase Based on an Enzyme-Free Electrocatalytic Reaction

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