Sanger-based sequencing technology for yellow fever vaccine genetic quality control

Pestana, Cristiane Pinheiro; Lawson-Ferreira, Rafael; Lessa-Aquino, Carolina; Leal, M; Freire, Marcos da Silva; Homma, Akira; Medeiros, Marco Alberto

doi:10.1016/j.jviromet.2018.07.006

Cited by 6 publications

(14 citation statements)

References 17 publications

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“…The technically uncovered regions of the 211 selected genes in the amplicon‐based next‐generation sequencing analysis were sequenced directly by Sanger sequencing. Only bases with quality values above 20 (<1% probability of the base being incorrect) were considered for further data analysis 39 …”

Section: Methodsmentioning

confidence: 99%

Targeted resequencing showing novel common and rare genetic variants increases the risk of asthma in the Chinese Han population

Liu

Deng

et al. 2021

Clinical Laboratory Analysis

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Background Although studies have identified hundreds of genetic variants associated with asthma risk, a large fraction of heritability remains unexplained, especially in Chinese individuals. Methods To identify genetic risk factors for asthma in a Han Chinese population, 211 asthma‐related genes were first selected based on database searches. The genes were then sequenced for subjects in a Discovery Cohort (284 asthma patients and 205 older healthy controls) using targeted next‐generation sequencing. Bioinformatics analysis and statistical association analyses were performed to reveal the associations between rare/common variants and asthma, respectively. The identified common risk variants underwent a validation analysis using a Replication Cohort (664 patients and 650 controls). Results First, we identified 18 potentially functional rare loss‐of‐function (LOF) variants in 21/284 (7.4%) of the asthma cases. Second, using burden tests, we found that the asthma group had nominally significant (p < 0.05) burdens of rare nonsynonymous variants in 10 genes. Third, 23 common single‐nucleotide polymorphisms were associated with the risk of asthma, 7/23 (30.4%) and 9/23 (39.1%) of which were modestly significant (p < 9.1 × 10−4) in the Replication Cohort and Combined Cohort, respectively. According to our cumulative risk model involving the modestly associated alleles, middle‐ and high‐risk subjects had a 2.0‐fold (95% CI: 1.621–2.423, p = 2.624 × 10−11) and 6.0‐fold (95% CI: 3.623–10.156, p = 7.086 × 10−12) increased risk of asthma, respectively, compared with low‐risk subjects. Conclusion This study revealed novel rare and common genetic risk factors for asthma, and provided a cumulative risk model for asthma risk prediction and stratification in Han Chinese individuals.

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Section: Methodsmentioning

confidence: 99%

Targeted resequencing showing novel common and rare genetic variants increases the risk of asthma in the Chinese Han population

Liu

Deng

et al. 2021

Clinical Laboratory Analysis

View full text Add to dashboard Cite

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“…The severe viscerotropic disease caused by YFV has a high mortality rate, ranging from 25% to 50% 13 - 15 . The yellow fever virus is one of the most critical arboviruses.…”

Section: Introductionmentioning

confidence: 99%

“…In more severe cases, internal hemorrhage, kidney failure, shock, coma or even death can occur 11 . In the most severe cases, the mortality rate is between 20-50% 15 . Genetic factors seem to offer some protection, but according to Monath and Vasconcelos 11 , more studies are required to prove this hypothesis.…”

Section: Introductionmentioning

confidence: 99%

“…Even though there are some widely used biomarkers to monitor the renal function in chronic diseases such as the levels of urea, creatinine, cystatin C, inulin, iothalamate and chromium-EDTA 45 , 46 , they are not helpful in yellow fever, because of the appalling disease severity and progression. According to the WHO 33 , if there is hepato-renal failure, up to 50% of the patients will die within 7 to 10 days, and those who do not die, may experience a long period of recovery from liver and kidney damages 15 . Consequently, these data highlights the importance of more studies on the kidney involvement in YF disease, because they are very important organs that are indirect targets of the disease, with severe consequences.…”

Section: Introductionmentioning

confidence: 99%

“…It is only a palliative action in cases of low amounts of the vaccine and during outbreaks 53 , 54 . For this reason, the WHO has been stimulating new discoveries using viral genome sequencing to improve vaccines 15 , and some studies have shown that polyclonal antibody therapies are very effective against many viruses 55 .…”

Section: Introductionmentioning

confidence: 99%

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