Sanghuangporus sanghuang extract extended the lifespan and healthspan of Caenorhabditis elegans via DAF-16/SIR-2.1

Abstract

Sanghuangporus Sanghuang is a fungus species. As a traditional Chinese medicine, it is known for antitumor, antioxidant and anti-inflammatory properties. However, the antiaging effect of S. Sanghuang has not been deeply studied. In this study, the effects of S. Sanghuang extract (SSE) supernatants on the changes of nematode indicators were investigated. The results showed that different concentrations of SSE prolonged the lifespans of nematodes and substantially increased these by 26.41%. In addition, accumulations of lipofuscin were also visibly reduced. The treatment using SSE also played a role in increasing stress resistance, decreasing ROS accumulations and obesity, and enhancing the physique. RT–PCR analysis showed that the SSE treatment upregulated the transcription of daf-16, sir-2.1, daf-2, sod-3 and hsp-16.2, increased the expression of these genes in the insulin/IGF-1 signalling pathway and prolonged the lifespans of nematodes. This study reveals the new role of S. Sanghuang in promoting longevity and inhibiting stress and provides a theoretical basis for the application of S. Sanghuang in anti-ageing treatments.

Anti-aging effect of glycerophosphocholine in Steinernema kraussei 0657L

“…The accumulation of ROS is considered to be one of the important causes of aging in C. elegans ( Dong et al, 2023 ). The method of C. elegans was used for the determination of ROS levels in S. kraussei cells, and H 2 DCFH-DA was also used for the determination of ROS levels in S. kraussei cells.…”

“…The mitochondrial free radical theory of aging (MFRTA) is one of the most important theories of aging and proposes that aging is caused by the destruction of macromolecules by mitochondrial ROS. Therefore, ROS accumulation is considered to be one of the important causes of senescence, and decreasing ROS production can effectively delay aging (Dong et al, 2023). Normally, with increased age, ROS cause oxidative damage to DNA, proteins and lipids, ROS levels rise, damage accumulates, cell dysfunction occurs, and Frontiers in Physiology frontiersin.org the aging process accelerates, which leads to a shortened lifespan (Qi et al, 2021).…”

“…The insulin signaling pathway is involved in metabolism and growth, and the core players in this pathway are insulin-like peptides, including insulin in mammals, IGF-I and IGF-II, phosphorylation of insulin receptor (IR) insulin receptor substrate proteins (IRS proteins) in the presence of insulin and its peptide-like proteins, and phosphorylation of insulin receptor (IR) insulin receptor substrate proteins (IRS proteins) ( Partridge et al, 2011 ). These proteins are primarily involved in the activation of two signaling pathways: the phosphatidylinositol 3-kinase (PI3K-Akt/protein kinase B (PKB) pathway, which is responsible for most insulin metabolism; And the RAS-mitogen-activated protein kinase (MAPK) pathway, which regulates the expression of some genes and works with the PI3K pathway to control cell growth and differentiation ( Taniguchi et al, 2006 ; Okamoto et al, 2013 ; Dong et al, 2023 ). As the same family of EPNs, C. elegans is mature in anti-aging research and can be used as a reference.…”

Research Progress of Bioactive Components in Sanghuangporus spp.

“…By treating the Caenorhabditis elegans nematodes with 10, 25, and 50 mg/mL of S. sanghuang extracts to study their effects on lifespan in vivo under the influence of 50 μM 5-fluoro-20-deoxyuridine (FUDR), Dong et al discovered that, after 14 days, the 25 mg/mL group showed a 26.41% increase in the mean lifespan compared to the control group (20.52 ± 0.34 days and 16.23 ± 0.36 days, respectively) [ 239 ]. They also inferred that the antiaging effect was achieved at the mRNA level, such as increased transcriptional regulators of daf-16 and sir-2.1, and that the lifespan was prolonged through the insulin/IGF-1 signaling pathway [ 239 ]. Their results, for the first time, revealed the related mechanisms in the antiaging effect of Sanghuang components.…”