Sanitary Status of the Grapevine Germplasm Collection in Republic of Srpska

Abstract

In July 2015, 179 grapevine plants belonging to 16 grapevine autochthonous cultivars were assessed for sanitary status using DAS ELISA test for the presence of: Grapevine fanleaf virus (GFLV), Grapevine leafrollassociated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine leafroll-associated virus 3 (GLRaV-3). Furthermore, survey for the phytoplasma presence and laboratory analyses using nested-PCR/RFLP assay was conducted at the beginning of September 2015 on grapevine cultivars which were not positive in DAS ELISA test for the presence of the four viruses. Out of 179 tested plants with DAS ELISA test, 146 (81%) were positive for the presence of at least one virus. The most widespread viruses were GFLaV-1 and GFLaV-3 with approximately 80 % of grapevines infected. Nested-PCR/RFLP assay showed that out of 33 tested samples 2 were positive for the presence of phytoplasmas from 16SrXII group. Sanitation of infected grapevine cultivars is needed in near future.

Survey of five major grapevine viruses infecting Blatina and Žilavka cultivars in Bosnia and Herzegovina

“…The dominant virus found in our study was GLRaV-3, a result that is in agreement with previous studies in BiH on both cultivars [ 20 , 61 , 64 , 65 ]. Similar reports have also been published in neighbouring Balkan countries.…”

“…These symptoms indicate the presence of grapevine leafroll-associated viruses (GLRaV-1 and -3), and in particular GLRaV-3, which resulted to be the most prevalent virus in both single and mixed infections. The symptoms here observed fit with those described by Delić et al [ 61 ] on the same cultivars affected by GLD from one vineyard in BiH. The observed delayed growth, leaves with distortions and asymmetry, and shortening of the internodes, all of which are associated with GFDD, were similar to those previously described for GFLV involvement in this disease [ 62 ].…”