Şap virüsü şuşlarına karşı poliklonal antikor üretimi

Abstract

Antibodies (Abs) have been one of the most important tools in diagnostic laboratories. Many diagnostic techniques such as Enzyme-Linked Immuno-Sorbent Assays (ELISA), immunofluorescence, Ab-microarray platforms, immunoblots, X-ray crystallography require the Abs. ELISA is an assay method that is among the basic tests using antibodies for the serology of Foot and Mouth Disease (FMDV). This method requires polyclonal or monoclonal antibodies to detect FMDV antigen or antibodies. For this purpose, solid-phase competitive ELISA (SPCE) or liquid phase blocking ELISA (LPBE) and non-structural protein (NSP) ELISA are used. SPCE and LPBE have mainly used FMDV structural protein antibody (SP-Ab) detection.In this study, it was aimed to produce a polyclonal Ab against FMDV ANep84 (Genotype VII) and OTUR07 (OPanAsia II), ATUR11 (A Iran05) strains for LPBE, FMDV SP-Ab detection. For this purpose, four guinea pigs and six rabbits were used for each serotype of FMDV. After producing Abs, checkerboard ELISA titration was performed to determine the optimal test dilution of Abs. Backgrounds, cross-reactions against three strains of FMDV were also checked. In conclusion, polyclonal Abs were produced against FMDV ANep84 (Genotype VII) and O Tur07 (O Pan Asia II), ATUR11 (A Iran05) strains, and standardized for LPBE test.