Saponified waste palm oil as an attractive renewable resource for mcl-polyhydroxyalkanoate synthesis

Pulsed feeding strategy is more favorable to medium‐chain‐length polyhydroxyalkanoates production from waste rapeseed oil

“…The obtained data confirmed that the pulsed feeding strategy was more effective than the continuous culture at producing mcl‐PHAs. The same situation was observed in our previous study when waste palm oil was applied as the only carbon source . Surprisingly, these results are inconsistent with the data of Chen et al who used fermented volatile fatty acids (VFA) as a substrate for the scl‐PHAs production by mixed culture selected from activated sludge.…”

“…However, it was observed that nitrogen limitation is unnecessary for the synthesis of significant amounts of mcl‐PHAs in Pseudomonas sp. Gl01 grown on saponified waste palm oil . This suggests that the need for unbalanced nutrient conditions to stimulate biopolymer accumulation depends on the carbon source.…”

“…Gl01 isolated from the mixed microbial community and identified at the Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, Poland . The medium composition has been reported previously . Cultures were prepared by first growing the strain overnight in Luria‐Bertani medium.…”

Plant Oils and Products of Their Hydrolysis as Substrates for Polyhydroxyalkanoate Synthesis

“…Saponified palm kernel oil has been used to produce mcl-PHA (3 g L -1 CDW 37 % PHA) in Pseudomonas putida PGA1 21,22 . Mcl-PHA has been accumulated by Pseudomonas aeruginosa (3.5-5.5 g L -1 CDW, 66.1 % PHA) from waste free fatty acids from soybean oil 13 and from saponified waste palm oil by Pseudomonas sp G101 (5.2 g L -1 CDW, 42 % PHA) 23 .…”

Utilization of Waste Carbon Source as Substrate and Economical Production of Biopolymer Polyhydroxyalkanoate Through Bacterial Consortia

“…Moreover, the use of waste vegetable oils seems to be profitable during the fermentations of Pseudomonas species. Możejko and Ciesielski (2013) reported that the newly isolated Gl01 strain belonging to the Pseudomonas species was capable of producing up to 48% mcl-PHA at 17 hrs from the culture using saponified palm oil as source of carbon. A high concentration of PHAs was reached by Możejko et al (2011Możejko et al ( , 2012) during culture of Cupriavidus necator using waste rapeseed oil which was supplied as a sole substrate in the bioreactor.…”