Saponins from Saffron Corms Inhibit the Gene Expression and Secretion of Pro-Inflammatory Cytokines

Keller, Morris; Fankhauser, Sarah C.; Giezendanner, Noreen; Keresztes, F; Danton, Ombeline; Fertig, Orlando; Marcourt, Laurence; Hamburger, Matthias; Butterweck, Veronika; Potterat, Olivier

doi:10.1021/acs.jnatprod.0c01220

Cited by 6 publications

(3 citation statements)

References 46 publications

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“…It has been demonstrated that the water solubility of EA is poor (41)(42)(43). It has been well established that poor water solubility leads to low bioavailability, which nally limits the clinical application.…”

Section: Discussionmentioning

confidence: 99%

Echinocystic acid induces the apoptosis, and inhibits the migration and invasion of non-small cell lung cancer cells

Zhu

Wang

et al. 2023

Med Oncol

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An increasing amount of evidence has demonstrated the anticancer activity of triterpenes extracted from traditional medicines. Echinocystic acid (EA), a natural triterpene isolated from Eclipta prostrata (L.) L., has previously been shown to exhibit anticancer activity in HepG2 and HL-60 cells. The aim of the present study was to investigate the anticancer activity of EA in non-small cell lung cancer (NSCLC) cells. For this purpose, the viability and proliferation of A549 cells were determined using a Cell Counting Kit-8 and 5ethynyl-2'-deoxyuridine staining. The migratory and invasive ability of the A549 cells were measured using wound healing and Transwell assays. Hoechst staining was also performed to detect the apoptosis of A549 cells. The proliferation of A549 cells and the distributions of different growth phases were determined using a ow cytometer. Western blot analysis was used to detect the expression levels of cyclin D, partitioning defective 3 homolog (Par3), PI3K, Akt, mTOR, Bax, Bcl-2 and caspase-3. EA inhibited the proliferation, and the migratory and invasive abilities of cultured lung carcinoma cells (A549 cells), and induced cell cycle arrest in the G 1 phase of the cell cycle. Treatment with EA upregulated Par3 expression and inhibited the PI3K/Akt/mTOR pathway in vitro. In addition, EA treatment inhibited tumor growth, suppressed proliferation and induced the apoptosis of tumor cells in NSCLC tumor xenografts in mice. On the whole, these results suggest that EA may represent a potential therapeutic agent for NSCLC.

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Section: Discussionmentioning

confidence: 99%

Echinocystic acid induces the apoptosis, and inhibits the migration and invasion of non-small cell lung cancer cells

Zhu

Wang

et al. 2023

Med Oncol

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show abstract

Section: Discussionmentioning

confidence: 99%

Echinocystic acid induces the apoptosis, and inhibits the migration and invasion of non-small cell lung cancer cells

Zhu

Wang

Jiang

et al. 2022

Preprint

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An increasing amount of evidence has demonstrated the anticancer activity of triterpenes extracted from traditional medicines. Echinocystic acid (EA), a natural triterpene isolated from Eclipta prostrata (L.) L., has previously been shown to exhibit anticancer activity in HepG2 and HL-60 cells. The aim of the present study was to investigate the anticancer activity of EA in non-small cell lung cancer (NSCLC) cells. For this purpose, the viability and proliferation of A549 cells were determined using a Cell Counting Kit-8 and 5-ethynyl-2’-deoxyuridine staining. The migratory and invasive ability of the A549 cells were measured using wound healing and Transwell assays. Hoechst staining was also performed to detect the apoptosis of A549 cells. The proliferation of A549 cells and the distributions of different growth phases were determined using a flow cytometer. Western blot analysis was used to detect the expression levels of cyclin D, partitioning defective 3 homolog (Par3), PI3K, Akt, mTOR, Bax, Bcl-2 and caspase-3. EA inhibited the proliferation, and the migratory and invasive abilities of cultured lung carcinoma cells (A549 cells), and induced cell cycle arrest in the G1 phase of the cell cycle. Treatment with EA upregulated Par3 expression and inhibited the PI3K/Akt/mTOR pathway in vitro. In addition, EA treatment inhibited tumor growth, suppressed proliferation and induced the apoptosis of tumor cells in NSCLC tumor xenografts in mice. On the whole, these results suggest that EA may represent a potential therapeutic agent for NSCLC.

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“…MPLC and FC are operated with usually with 15-30 lm stationary phase particles (pressure: tens of bars) and semi-preparative HPLC with 5-10 lm particles (pressure: hundreds of bars) (Atanasov et al 2015;Bucar et al 2013;Sticher 2008). Semi-preparative HPLC has been mainly used for the final purification step of simplified fractions originating from the other preparative approaches (Angelis et al 2020;Darme et al 2022;Human et al 2021;Jing et al 2022;Keller et al 2021;Zhou et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Advanced high-resolution chromatographic strategies for efficient isolation of natural products from complex biological matrices: from metabolite profiling to pure chemical entities

Queiroz,

Guillarme,

Wolfender

2024

Phytochem Rev

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The isolation of pure compounds from extracts represents a key step common to all investigations of natural product (NP) research. Isolation methods have gone through a remarkable evolution. Current approaches combine powerful metabolite profiling methods for compounds annotation with omics mining results and/or bioassay for bioactive NPs/biomarkers priorisation. Targeted isolation of prioritized NPs is performed using high-resolution chromatographic methods that closely match those used for analytical profiling. Considerable progress has been made by the introduction of innovative stationary phases providing remarkable selectivity for efficient NPs isolation. Today, efficient separation conditions determined at the analytical scale using high- or ultra-high-performance liquid chromatography can be optimized via HPLC modelling software and efficiently transferred to the semi-preparative scale by chromatographic calculation. This ensures similar selectivity at both the analytical and preparative scales and provides a precise separation prediction. High-resolution conditions at the preparative scale can notably be granted using optimized sample preparation and dry load sample introduction. Monitoring by ultraviolet, mass spectrometry, and or universal systems such as evaporative light scattering detectors and nuclear magnetic resonance allows to precisely guide the isolation or trigger the collection of specific NPs with different structural scaffolds. Such approaches can be applied at different scales depending on the amounts of NPs to be isolated. This review will showcase recent research to highlight both the potential and constraints of using these cutting-edge technologies for the isolation of plant and microorganism metabolites. Several strategies involving their application will be examined and critically discussed. Graphical abstract

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Saponins from Saffron Corms Inhibit the Gene Expression and Secretion of Pro-Inflammatory Cytokines

Cited by 6 publications

References 46 publications

Echinocystic acid induces the apoptosis, and inhibits the migration and invasion of non-small cell lung cancer cells

Echinocystic acid induces the apoptosis, and inhibits the migration and invasion of non-small cell lung cancer cells

Echinocystic acid induces the apoptosis, and inhibits the migration and invasion of non-small cell lung cancer cells

Advanced high-resolution chromatographic strategies for efficient isolation of natural products from complex biological matrices: from metabolite profiling to pure chemical entities

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