Saponins isolated from the root of Platycodon grandiflorum protect against acute ethanol-induced hepatotoxicity in mice

Khanal, Tilak; Choi, Jae Ho; Hwang, Yong Pil; Chung, Young Chul; Jeong, Hye Gwang

doi:10.1016/j.fct.2008.12.009

Cited by 62 publications

(45 citation statements)

References 38 publications

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“…The leakage of ALT, AST and LDH in culture medium was measured in order to demonstrate the hepatoprotective activity of GTs. As shown in Figure 3(A-C), t-BHP challenge caused significant increase of ALT, AST and LDH leakage from the cytoplasm into culture medium in model group (p50.01), which was agreement with the observation (Khanal et al 2009). Pretreatment with various GTs decreased the level of ALT, AST and LDH in culture medium significantly (p50.05 or p50.01).…”

Section: Discussionsupporting

confidence: 77%

Hepatoprotective effect of ganoderma triterpenoids against oxidative damage induced bytert-butyl hydroperoxide in human hepatic HepG2 cells

Kan

et al. 2015

Pharmaceutical Biology

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Objective: The objective of this study is to better understand the hepatoprotective effect of GTs and to enhance their use in food supplement pharmaceutical and medical industries. Materials and methods: HepG2 cells were pretreated in the presence or absence of GTs (50, 100 and 200 mg/ml) for 4 h, then exposed with 60 mmol/L of t-BHP for an additional 4 h. The cell viability was evaluated by MTT method. ALT, AST and LDH production in culture medium and intracellular MDA, GSH and SOD levels were determined. Moreover, the total triterpenoid content and chemical constituents in GTs were detected by ultraviolet spectrophotometry and HPLC/Q-TOF-MS, respectively. Results: GTs (50, 100 and 200 mg/ml) significantly increased the relative cell viability by 4.66, 7.78 and 13.46%, respectively, and reduced the level of ALT by 11.44%, 33.41% and 51.24%, AST by 10.05%, 15.63% and 33.64%, and LDH by 16.03%, 23.4% and 24.07% in culture medium, respectively. GTs could also remarkably decrease the level of MDA and increase the content of GSH and SOD in HepG2 cells. Furthermore, the total triterpenoid content in GTs was 438 mg GAAEs/g GTs. And 16 triterpenoids in GTs were identified or tentatively characterised. Discussion and conclusion: Our results showed that GTs had potent cytoprotective effect against oxidative damage induced by t-BHP in HepG2 cells, thus suggesting their potential use as liver protectant.ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 77%

Hepatoprotective effect of ganoderma triterpenoids against oxidative damage induced bytert-butyl hydroperoxide in human hepatic HepG2 cells

Kan

et al. 2015

Pharmaceutical Biology

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“…However, TNF-α in BDL-induced cholestasis mediates acute and chronic liver injury and plays an important role in hepatic fibrosis and cirrhosis (Gäbele et al, 2009). Khanal et al (2009) reported that saponins isolated from the root of P. grandiflorum decreased the TNF-α level on acute ethanol-induced hepatic injury in mice.…”

Section: Discussionmentioning

confidence: 99%

“…These saponins exhibit a variety of pharmacological activities, such as anti-inflammatory, anticancer, and immune enhancing effects Shin et al, 2009;Xie et al, 2009). These saponins are believed to have protective effects against some chemical-induced hepatotoxicity (Khanal et al, 2009;Lee et al, 2001). However, the protective effect of the aqueous extract from P. grandiflorum has not been reported against biliary obstruction-induced injury in the liver.…”

Section: Research Articlementioning

confidence: 99%

Protective effect of the aqueous extract from the root ofPlatycodon grandiflorumon cholestasis-induced hepatic injury in mice

Kim

Lee

Song

et al. 2012

Pharmaceutical Biology

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“…For these reasons, it has been assumed that the EtOH-induced oxidative stress can be regulated by antioxidant potentials. While the protective effects of PG against EtOH through blocking CYP2E1-mediated EtOH bioactivation and AMP-dependent protein kinase activation, resulting in fatty liver inhibition, have been reported [11,12], it has not yet been explored whether the antioxidant effect of PG is related to these protective effects against chronic EtOH-induced liver damage. Therefore, the current study was performed to investigate whether PG root extract and its saponin fractions are protective in EtOH-treated liver cells and in an animal model, via the antioxidant defense mechanism against EtOH-induced oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, we have recently reported a fatty liver inhibition effect of PG extract and its saponin via suppression of hepatic lipogenesis and acceleration of energy expenditure, along with the modulation of liver fatty acid synthase and carnitine palmitoyltransferase activities in high-fat-diet-fed C57BL/6 mice [7]. Moreover, hepatoprotective roles of PG have been demonstrated in various chemical- [8,9,10] and ethanol (EtOH)-induced hepatotoxicity animal models [11,12]. Acute and chronic EtOH treatments increase the production of reactive oxygen substance, reduce cellular antioxidant levels and enhance oxidative stress in many tissues, especially in the liver [13].…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective Effect of <i>Platycodon grandiflorum</i> against Chronic Ethanol-Induced Oxidative Stress in C57BL/6 Mice

Noh

Kim²,

Gang³

et al. 2011

Ann Nutr Metab

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Aims: This study was carried out to evaluate the hepatoprotective effect of Platycodon grandiflorum (PG) in ethanol (EtOH)-induced liver damage. Methods and Results: PG treatment (both the total extract and saponin fraction) significantly blocked EtOH-induced oxidative stress through the preservation of activities of antioxidant enzymes in HepG2 cells. Furthermore, while the administration of EtOH to C57BL/6 mice for 6 weeks induced liver damage, along with a significant increase in plasma glutamic oxalacetic transaminase, glutamic pyruvic transaminase, hepatic triglyceride and thiobarbituric acid reactive substance levels, PG treatment significantly decreased glutamic oxalacetic transaminase, glutamic pyruvic transaminase, hepatic triglyceride and thiobarbituric acid reactive substance levels compared with the EtOH-treated control group (p < 0.05). Histological observation by hematoxylin-eosin and oil red O staining in the liver showed more effective inhibition of lipid accumulation in PG-treated groups, as compared to the EtOH-treated control group. Additionally, PG treatments appeared to enhance the activities of superoxide dismutase and catalase in the liver (p < 0.05). Conclusion: These results suggest that PG has a protective effect against EtOH-induced oxidative damage, possibly by inhibition of lipid accumulation and peroxidation through the enhancement of the antioxidant defense system. PG might be useful as a therapeutically potent natural ingredient for the prevention of chronic EtOH-induced oxidative stress and liver damage.

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Saponins isolated from the root of Platycodon grandiflorum protect against acute ethanol-induced hepatotoxicity in mice

Cited by 62 publications

References 38 publications

Hepatoprotective effect of ganoderma triterpenoids against oxidative damage induced bytert-butyl hydroperoxide in human hepatic HepG2 cells

Hepatoprotective effect of ganoderma triterpenoids against oxidative damage induced bytert-butyl hydroperoxide in human hepatic HepG2 cells

Protective effect of the aqueous extract from the root ofPlatycodon grandiflorumon cholestasis-induced hepatic injury in mice

Hepatoprotective Effect of <i>Platycodon grandiflorum</i> against Chronic Ethanol-Induced Oxidative Stress in C57BL/6 Mice

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