Sustainable crop production requires an innovative approach due to increasing soil salinisation and decreasing freshwater availability. One promising strategy is the domestication of naturally salt-tolerant plant species with commercial potential. Sarcocornia fruticosa is a highly salt-tolerant halophyte, common in Mediterranean marshes, which may hold promise for biosaline agriculture. This study included 11 populations of this species spread over the territory of the Valencian Community in eastern Spain. Climatic data for each locality were obtained from the nearest meteorological stations. Soil analyses included texture, pH, electroconductivity, organic carbon and organic matter. Biochemical analyses on wild-sampled plant material focused on antioxidant compounds, such as carotenoids, phenolics, flavonoids and proline with malondialdehyde (MDA) used as a marker of oxidative stress. All variables (climatic, edaphic and biochemical) were evaluated together using Principal Component Analysis and Spearman correlation. The results obtained indicated some climatic differences in terms of mean annual precipitation, with a clear N-S gradient and considerable edaphic variability. However, none of the environmental conditions showed a clear correlation with plant biochemical characteristics. Significant differences in the levels of phenolic compounds, flavonoids and MDA between populations were probably due to genetic factors and cannot be explained as a response to environmental conditions.