Sarcocystosis in a Stillborn Lamb

Agerholm, Jørgen Steen; Dubey, J. P.

doi:10.1111/rda.12398

Cited by 4 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although S. tenella was demonstrated to cause a severe disease in sheep (Munday et al 1975 ; Caldow et al 2000 ; Schock et al 2012 ; Agerholm and Dubey 2014 ), we cannot speculate on a potential pathogenicity of this species in Tatra chamois due to a small number of examined cadaveric specimens and the fact that we found only few microscopic cysts in the analyzed tissues.…”

Section: Discussionmentioning

confidence: 63%

“…While Sarcocystis spp. were demonstrated as a cause of enzootic muscle tissue parasitoses in other ruminants, with significant losses in livestock due to abortions, reduced weight, neurological disease, and even mortality in rarely observed acute cases (Caldow et al 2000 ; Schock et al 2012 ; Agerholm and Dubey 2014 ), we still lack a data on the prevalence and pathogenic role of Sarcocystis spp. in Tatra chamois.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

First molecular characterization of Sarcocystis tenella in Tatra chamois (Rupicapra rupicapra tatrica) in Poland

et al. 2015

View full text Add to dashboard Cite

In this study, sarcocysts from three Polish Tatra chamois were isolated and identified using morphological and molecular methods for the first time. Six cysts were found in the latissimus dorsi muscle and another two in the diaphragm. No sarcocysts were detected in the myocardium, tongue, and esophagus. The isolated cysts were long with rounded ends, 0.35–0.61 mm in length, and 0.02–0.06 mm in width. All the sarcocysts were identified as Sarcocystis tenella on the basis of light microscopy and sequencing of cytochrome C oxidase subunit I (cox1) and small-subunit rRNA (ssu rRNA) genes. Comparative analysis showed a 99.23 % identity of the cox1 gene sequences from Tatra chamois and sheep sarcocysts, and an even higher degree of sequence identity (99.88 %) was documented in the case of the ssu rRNA gene. When compared at a haplotype level, all the sheep sequences of cox1 differed from those isolated from Tatra chamois. In contrast, one out of the two ssu rRNA haplotypes from the sheep isolates was identical with the haplotype from Tatra chamois. In conclusion, we showed that cox1 and ssu rRNA genes can be used as genetic markers for identification of the S. tenella, with cox1 gene providing better resolution during phylogenetic analyses. However, both genetic population analysis and phylogenetic inference with cox1 and ssu rRNA genes demonstrated that they do not constitute good markers for spatial differentiation of S. tenella.Electronic supplementary materialThe online version of this article (doi:10.1007/s00436-015-4619-4) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

First molecular characterization of Sarcocystis tenella in Tatra chamois (Rupicapra rupicapra tatrica) in Poland

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Although no specific assay was performed to confirm or rule out the presence of Sarcocystis spp., schizonts containing merozoites arranged in a “rosette pattern” are usually histologically evident in fetal tissues, particularly in glomerular capillaries, endothelial cells of the brain and lungs ( 79 – 81 ). No schizonts resembling Sarcocystis spp.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the PCR for Apicomplexa Coccidia was only positive in cases that were also PCR-positive for T. gondii , but in none of the T. gondii -negative cases, suggesting that no other Apicomplexa Coccidia were involved. Sarcocystis appears to be an extremely infrequent cause of abortion in sheep, since it has been scarcely reported ( 79 – 81 ).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Investigation of 100 Cases of Abortion in Sheep in Uruguay: 2015–2021

et al. 2022

View full text Add to dashboard Cite

The aim of this work was to identify causes of abortion through laboratory investigations in sheep flocks in Uruguay. One hundred cases of abortion, comprising 58 fetuses, 36 fetuses with their placentas, and 6 placentas were investigated in 2015–2021. Cases were subjected to gross and microscopic pathologic examinations, and microbiological and serological testing for the identification of causes of abortion, including protozoal, bacterial, and viral pathogens. An etiologic diagnosis was determined in 46 (46%) cases, including 33 (33%) cases caused by infectious pathogens, as determined by the detection of a pathogen along with the identification of fetoplacental lesions attributable to the detected pathogen. Twenty-seven cases (27%) were caused by Toxoplasma gondii, 5 (5%) by Campylobacter fetus subspecies fetus, and 1 (1%) by an unidentified species of Campylobacter. Fourteen cases (14%) had inflammatory and/or necrotizing fetoplacental lesions compatible with an infectious etiology. Although the cause for these lesions was not clearly identified, T. gondii was detected in 4 of these cases, opportunistic bacteria (Bacillus licheniformis, Streptococcus sp.) were isolated in 2 cases, and bovine viral diarrhea virus 1 subtype i (BVDV-1i) was detected in another. Campylobacter jejuni was identified in 1 (1%) severely autolyzed, mummified fetus. BVDV-2b was identified incidentally in one fetus with an etiologic diagnosis of toxoplasmosis. Microscopic agglutination test revealed antibodies against ≥1 Leptospira serovars in 15/63 (23.8%) fetuses; however, Leptospira was not identified by a combination of qPCR, culture, fluorescent antibody testing nor immunohistochemistry. Neospora caninum, Chlamydia abortus, Chlamydia pecorum, Coxiella burnetii and border disease virus were not detected in any of the analyzed cases. Death was attributed to dystocia in 13 (13%) fetuses delivered by 8 sheep, mostly from one highly prolific flock. Congenital malformations including inferior prognathism, a focal hepatic cyst, and enterohepatic agenesis were identified in one fetus each, the latter being the only one considered incompatible with postnatal life. Toxoplasmosis, campylobacteriosis and dystocia were the main identified causes of fetal losses. Despite the relatively low overall success rate in establishing an etiologic diagnosis, a systematic laboratory workup in cases of abortion is of value to identify their causes and enables zoonotic pathogens surveillance.

show abstract

Diseases of the musculoskeletal system

Baird¹,

Shipley²

2021

Sheep, Goat, and Cervid Medicine

View full text Add to dashboard Cite

Sarcocystosis in a Stillborn Lamb

Cited by 4 publications

References 13 publications

First molecular characterization of Sarcocystis tenella in Tatra chamois (Rupicapra rupicapra tatrica) in Poland

First molecular characterization of Sarcocystis tenella in Tatra chamois (Rupicapra rupicapra tatrica) in Poland

Diagnostic Investigation of 100 Cases of Abortion in Sheep in Uruguay: 2015–2021

Diseases of the musculoskeletal system

Contact Info

Product

Resources

About