Sarcoglycan subcomplex in normal human smooth muscle: An immunohistochemical and molecular study

Anastasi, Giuseppe; Cutroneo, Giuseppina; Sidoti, Antonina; Santoro, Giuseppe; D’Angelo, Rosalia; Rizzo, Giuseppina; Rinaldi, Carmen; Giacobbe, O.; Bramantı, Placido; Navarra, Giuseppe; Amato, Aldo; Favaloro, Angelo

doi:10.3892/ijmm.16.3.367

Cited by 16 publications

(19 citation statements)

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“…This point was partially clarified in our previous immunohistochemical and molecular investigation, carried out on only human adult smooth muscle of gastrointestinal tract, in which we demonstrated the presence of a pentameric arrangement around the SGC (Anastasi et al 2005).…”

Section: Sarcoglycan In Normal Human Smooth Musclementioning

confidence: 98%

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Sarcoglycan Subcomplex Expression in Normal Human Smooth Muscle

Anastasi

Cutroneo

Sidoti

et al. 2007

J Histochem Cytochem.

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The sarcoglycan complex (SGC) is a multimember transmembrane complex interacting with other members of the dystrophin–glycoprotein complex (DGC) to provide a mechanosignaling connection from the cytoskeleton to the extracellular matrix. The SGC consists of four proteins (α, β, γ, and δ). A fifth sarcoglycan subunit, ∊-sarcoglycan, shows a wider tissue distribution. Recently, a novel sarcoglycan, the ζ-sarcoglycan, has been identified. All reports about the structure of SGC showed a common assumption of a tetrameric arrangement of sarcoglycans. Addressing this issue, our immunofluorescence and molecular results showed, for the first time, that all sarcoglycans are always detectable in all observed samples. Therefore, one intriguing possibility is the existence of a pentameric or hexameric complex considering ζ-sarcoglycan of SGC, which could present a higher or lower expression of a single sarcoglycan in conformity with muscle type—skeletal, cardiac, or smooth—or also in conformity with the origin of smooth muscle. (J Histochem Cytochem 55:831–843, 2007)

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Section: Sarcoglycan In Normal Human Smooth Musclementioning

confidence: 98%

“…Based on these findings, we hypothesized the presence of a pentameric structure of SGC and not a conventional heterotetrameric unit (Anastasi et al 2005).…”

mentioning

confidence: 99%

Sarcoglycan Subcomplex Expression in Normal Human Smooth Muscle

Anastasi

Cutroneo

Sidoti

et al. 2007

J Histochem Cytochem.

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“…Our previous immunohistochemical and molecular studies on many sites of the human body, demonstrated that all sarcoglycans, with higher or lower expression, are expressed in human smooth muscle fibers hypothesizing the intriguing possibility of the existence of a pentameric arrangement of the SGC. The higher or lower expression of sarcoglycan subunits could be in conformity with the location and function of smooth muscle (14,27).…”

Section: Discussionmentioning

confidence: 99%

Abnormal distribution of sarcoglycan subcomplex in colonic smooth muscle cells of aganglionic bowel

Favaloro¹

2010

Int J Mol Med

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Abstract. Hirschsprung's disease (HD) is a development disorder of the enteric nervous system in which the altered innervation explains the inability of the aganglionic segment to relax. Impairment of cytoskeleton in SMC of aganglionic bowel has been shown. Sarcoglycan subcomplex (SG) may support the development and maintenance of muscle cells. We examined the SG subunit expression in colonic aganglionic and ganglionic specimens obtained from patients with HD. Full-thickness bowel specimens were obtained from six patients with HD. Six normal colon specimens were used as controls. Immunofluorescent analysis and reverse transcriptase polymerase chain reaction evaluation were performed for ·-, ß-, Á-, ‰-and ε-SG. In control colon, the indirect immunofluorescence showed a strong staining pattern of ß-Á-‰-and ε-SG while a weak positivity of ·-SG was recorded. In aganglionic bowel, immunofluorescence intensity values documented a significant lack of ε-SG while an enhanced ·-SG, coupled to a loss of ε-SG, was recorded in ganglionic bowel in HD-affected patients. Our observations underscore the assumption that non-neuronal elements of the colon might play a key role in the pathogenesis of HD and loss of Â-SG might critically alter the cytoskeleton in the aganglionic bowel segment. Up-regulation of ·-SG is probably an acquired phenomenon to reinforce the sarcolemma and to perform a forceful contraction in dilated ganglionic HDaffected colon, related to chronic pseudo-obstruction, contributing to the intestinal dysmotility that persists in 20% of patients after resection of the aganglionic bowel.

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confidence: 99%

“…ε-sarcoglycan may substitute for α-sarcoglycan in a subset of striated muscle complexes. Our recent study on smooth muscle fibers, hypothesized an exameric structure of SGC (Anastasi et al, 2005).The sarcoglycans play a key role in the pathogenesis of many muscular dystrophies, such as Duchenne and Becker muscular dystrophies and sarcoglycanopathies (Bönnemann et al, 2002). In fact, recent developments in molecular genetics have demonstrated that mutation in each single sarcoglycan gene, respectively 17q, 4q, 13q and 5q, causes a series of recessive autosomal dystrophinpositive muscular dystrophies, not accompanied by a lack of dystrophin, called sarcoglycanopathies or Limb Girdle Muscular dystrophies (LGMD type 2D, 2E, 2C and 2F) (Roberds et al, 1994).…”

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The Fathers of Italian Histology

ISH¹

2009

Eur J Histochem

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Subscriptions, cancellations, business correspondence and any enquiries must be sent to the Tipografia PIME Editrice srl, Pavia, Italy. Cancellations must be received before the end of September to take effect at the end of the same year.No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means (electronic, electrostatic, magnetic type, mechanical, photocopying or otherwise) without written permission by the Publishers.Reg. Tribunale di Pavia n. 289/23.2.1984. Supported by the Ministero per i Beni e le Attività Culturali, Italy as a publication of high cultural value.Associato all'USPI Unione Stampa Periodica Italiana Disclaimer. Whilst every effort is made by the publishers and the editorial board to see that no inaccurate or misleading data, opinion or statement appears in this journal, they wish to make it clear that the data and opinions appearing in the articles or advertisements herein are the responsibility of the contributor or advisor concerned. Accordingly, the publisher, the editorial board and their respective employees, officers and agents accept no liability whatsoever for the consequences of any inaccurate or misleading data, opinion or statement. The Journal publishes original papers, technical reports, letters to the editor, review articles concerning investigations performed with the aid of biophysical, biochemical, molecular-biological, enzymatic, immunohistochemical, cytometric, and image analysis techniques. Areas of particular interest to the European Journal of Histochemistry include: -functional cell and tissue biology in animals and plants; -cell differentiation and death; -cell-cell interaction and molecular trafficking; -biology of cell development and senescence; -nerve and muscle cell biology; -cellular basis of diseases During this period, Carlo Rizzoli established the experimental approach for the study of the molecular basis of cell differentiation in vitro, anticipating some aspects of the present investigation on the potentiality of stem cells. Furthermore, Carlo Rizzoli was one of the first Italian scientists to publish its scientific reports in large-diffusion international journals, thus contributing to the world-wide diffusion of the seminal studies on the in vitro cell differentiation models. Editor-in-ChiefIn 1961, Carlo Rizzoli became Professor of Histology and general embryology and, since 1964 to 1999, Director of the Institute of Histology at the University of Bologna.The initial steps of this undertaking were challenging, since in 1963, following the recruitment of Oliviero Mario Olivo at the Chair of Human Anatomy, the facilities of the Institute of Histology were almost nonexistent. In few years, however, Carlo Rizzoli was able to organize an efficient research group of motivated young collaborators that included Paolo Carinci, Lia Guidotti, Francesco Antonio Manzoli, capable of introducing original and seminal lines of research into the national and international histological arena. In this way, a numb...

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Sarcoglycan subcomplex in normal human smooth muscle: An immunohistochemical and molecular study

Cited by 16 publications

References 17 publications

Sarcoglycan Subcomplex Expression in Normal Human Smooth Muscle

Sarcoglycan Subcomplex Expression in Normal Human Smooth Muscle

Abnormal distribution of sarcoglycan subcomplex in colonic smooth muscle cells of aganglionic bowel

The Fathers of Italian Histology

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