Sarcolemmal hydraulic conductivity of guinea-pig and rat ventricular myocytes

Ogura, Toshitsugu; Matsuda, Hiroyuki; Imanishi, Sunao; Shibamoto, Toshishige

doi:10.1016/s0008-6363(02)00267-5

Cited by 21 publications

(25 citation statements)

References 33 publications

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“…The AQP-1-dependent percentage of myocardial water permeability of between 20 and 30%, seen in membranes from knockout mice as well as from wild-type mice treated with HgCl 2 , is consistent with the estimated contribution of AQPs to total myocardial water flux determined by Kellen et al (14,15) in whole heart experiments, although less than that estimated from isolated cardiomyocytes in studies by Ogura et al (36,37). Importantly, water permeability assessed by Kellen et al (14,15) is the net effect of endothelium-and cardiomyocytederived AQPs, whereas that in isolated cells used by Ogura et al (36,37) would include only cardiomyocyte-derived AQPs. It is acknowledged that the three different models discussed above are not strictly comparable.…”

Section: Discussionsupporting

confidence: 88%

“…Both structures can be considered as sarcolemmal, either the invaginations of the cell membrane into the Z line in the case of the t-tubules or the pinched-off vesicles in the case of caveolae. AQPs appear to be present in isolated cardiac myocytes as demonstrated by measurement of sarcolemmal permeability (37).…”

Section: Discussionmentioning

confidence: 99%

“…Studies of isolated perfused rabbit heart (14) suggest that interendothelial cell clefts account for twothirds of water movement across the capillary bed and into the interstitial space, with one-third passing through a possible water channel pathway (15) that may involve AQP-1 in cardiac endothelial cells (3). Measurement of osmotically induced changes in cell volume by videomicroscopy in single cells has suggested the involvement of AQPs in water transport in guinea pig and rat heart cells (36,37), based on water transport rates and the inhibitory effect of Hg 2ϩ , a nonspecific, although toxic, AQP inhibitor. However, similar techniques using rabbit ventricular cardiomyocytes (44) suggested that membrane diffusion rather than water channels was the predominant path of water movement.…”

mentioning

confidence: 99%

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Cardiac aquaporin expression in humans, rats, and mice

Butler¹,

Au²,

Yang³

et al. 2006

American Journal of Physiology-Heart and Circulatory Physiology

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Water accumulation in the heart is important in ischemia-reperfusion injury and operations performed by using cardiopulmonary bypass, with cardiac dysfunction associated with myocardial edema being the principal determinant of clinical outcome. As an initial step in determining the role of aquaporin (AQP) water channels in myocardial edema, we have assessed the myocardial expression of AQPs in humans, rats, and mice. RT-PCR revealed expression of AQP-1, -4, -6, -7, -8, and -11 transcripts in the mouse heart. AQP-1, -6, -7, and -11 mRNAs were found in the rat heart as well as low levels of AQP-4 and -9. Human hearts contained AQP-1, -3, -4, -5, -7, -9, -10, and -11 mRNAs. AQP-1 protein expression was confirmed by Western blot analysis in all three species. AQP-4 protein was detected in the mouse heart but not in the rat or human heart. To determine the potential functional consequences of myocardial AQP expression, water permeability was measured in plasma membrane vesicles from myocardial cells of wild-type versus various AQP knockout mice. Water permeability was reduced by AQP-1 knockout but not by AQP-4 or AQP-8 knockout. With the use of a model of isolated rat heart perfusion, it was found that osmotic and ischemic stresses are not associated with changes in AQP-1 or AQP-4 expression. These studies support a possible functional role of AQP-1 in myocardium but indicate that early adaptations to osmotic and ischemic stress do not involve transcriptional or posttranslational AQP-1 regulation. aquaporin knockout; cardiac myocyte; cardiopulmonary bypass OUR INTEREST IN CARDIAC AQUAPORINS (AQPs) stems from the recognition that myocardial edema is associated with a significant reduction in cardiac performance in a number of clinically important situations. Ischemia and reperfusion are associated with the development of myocardial edema, with expansion of both interstitial and cellular compartments. The resulting systolic and diastolic dysfunctions are the most important prognostic factors in patient survival after an acute ischemic event or "planned" ischemia, as occurs during cardiac surgery. Procedures involving cardiopulmonary bypass bring additional osmotic stress due to hemodilution and increased vascular permeability (32,40,42

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Cardiac aquaporin expression in humans, rats, and mice

Butler¹,

Au²,

Yang³

et al. 2006

American Journal of Physiology-Heart and Circulatory Physiology

View full text Add to dashboard Cite

show abstract

“…Alternatively, water may be conveyed by other cardiac water pores in the absence of AQP4. We have presented evidence for the expression of AQP1 in human cardiomyocytes [55] and a mercury-sensitive form of water transport in rat cardiomyocytes may similarly be explained by the presence of AQP1 [62], also shown by immunofluorescent microscopy [54]. Neither knockout [63] nor overexpression [64] of AQP4 results in altered gross morphology of the mouse heart.…”

Section: Aquaporins In the Heartmentioning

confidence: 69%

Cardiac Aquaporins: Significance in Health and Disease

Butler

Winlaw

2011

Heart Rate and Rhythm

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“…However, the underlying pathophysiological mechanism of myocardial edema after severe burns has not been fully understood. Recent studies suggested that aquaporins (AQPs), which are crucial for regulation of cell volume, transepithelial water transport, and whole body homeostasis, may play a potential role in cardiac edema (5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

The expression profile of aquaporin 1 in rat myocardium after severe burns

Li¹,

Zha²,

Li³

et al. 2015

Turk J Med Sci

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Background/aim: To investigate the myocardial expression profile of aquaporin 1 (AQP1) in rats after severe burns.Materials and methods: Ninety healthy male adult Wistar rats were randomly assigned to the following treatments: sham operation (control group, n = 6), immediate fluid resuscitation treatment post scalding (IF group, n = 42), and delayed fluid resuscitation treatment after scalding (DF group, n = 42). At 3, 6, 12, 24, 48, 72, and 120 h after scalding, myocardial water contents were assayed and AQP1 expressions were detected by real-time polymerase chain reaction and western blot. The AQP levels in the myocardium at 12 h after scalding were assayed by gene microarrays.Results: Scald injuries resulted in significantly synchronized increases in the myocardial water contents and the myocardial mRNA and protein expression of AQP1, with a peak at 12 h after scalding. Rats receiving delayed fluid resuscitation treatment had more severe myocardial edema and significantly higher myocardial AQP1 expressions than the rats receiving immediate fluid resuscitation treatment. The mRNAs of 6 other AQPs (AQP2, 3, 4, 6, 7, and 12b) were found to be changed in the myocardium among rats with different treatments. Conclusion:AQP1 may play a functional role in the development of myocardial edema after scalding. Targeting AQP1 may provide opportunities for therapeutic intervention in myocardial edema following severe burns.

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Sarcolemmal hydraulic conductivity of guinea-pig and rat ventricular myocytes

Cited by 21 publications

References 33 publications

Cardiac aquaporin expression in humans, rats, and mice

Cardiac aquaporin expression in humans, rats, and mice

Cardiac Aquaporins: Significance in Health and Disease

The expression profile of aquaporin 1 in rat myocardium after severe burns

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