Sarcomere Structure: The Importance of Desmin Protein in Muscle Atrophy

Marzuca‐Nassr, Gabriel Nasri; Vitzel, Kaio Fernando; Mancilla-Solorza, Eladio; Márquez, José Luis

doi:10.4067/s0717-95022018000200576

Cited by 10 publications

(8 citation statements)

References 47 publications

(61 reference statements)

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“…This statement was supported by up-regulated DES and VIM in FG relative to those of MG at the later developmental stages. The two genes, also known as the markers for muscle regenerative process, encode intermediate filaments proteins contributing to the sarcomere integrity [ 72 ]. Consistent with our results, Soglia et al [ 14 ] recently reported that the breast muscles of a slow-growing strain expressed lower DES (p = 0.08) and VIM (p = 0.10) if compared with unaffected broilers.…”

Section: Discussionmentioning

confidence: 99%

Differential expression patterns of genes associated with metabolisms, muscle growth and repair in Pectoralis major muscles of fast- and medium-growing chickens

et al. 2022

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The aim of this study was to investigate the expression of genes related to muscle growth, hypoxia and oxidative stress responses, a multi-substrate serine/threonine-protein kinase (AMPK) and AMPK-related kinases, carbohydrate metabolism, satellite cells activities and fibro- adipogenic progenitors (FAPs) in fast-growing (FG) (n = 30) and medium-growing (MG) chickens (n = 30). Pectoralis major muscles were collected at 7d, 14d, 21d, 28d, 35d and 42d of age. According to their macroscopic features, the samples from FG up to 21d of age were classified as unaffected, while all samples collected at an older age exhibited macroscopic features ascribable to white striping and/or wooden breast abnormalities. In contrast, MG samples did not show any feature associated to muscle disorders. The absolute transcript abundance of 33 target genes was examined by droplet digital polymerase chain reaction. The results showed differential gene expression profiles between FG and MG chickens at different ages. While most genes remained unchanged in MG chickens, the expression patterns of several genes in FG were significantly affected by age. Genes encoding alpha 1, alpha 2, beta 2 and gamma 3 isoforms of AMPK, as well as AMPK-related kinases, were identified as differentially expressed between the two strains. The results support the hypothesis of oxidative stress-induced muscle damage with metabolic alterations in FG chickens. An increased expression of ANXA2, DES, LITAF, MMP14, MYF5 and TGFB1 was observed in FG strain. The results suggest the occurrence of dysregulation of FAP proliferation and differentiation occurring during muscle repair. FAPs could play an important role in defining the proliferation of connective tissue (fibrosis) and deposition of intermuscular adipose tissue which represents distinctive traits of muscle abnormalities. Overall, these findings demonstrate that dysregulated molecular processes associated with myopathic lesions in chickens are strongly influenced by growth rate, and, to some extent, by age.

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Section: Discussionmentioning

confidence: 99%

Differential expression patterns of genes associated with metabolisms, muscle growth and repair in Pectoralis major muscles of fast- and medium-growing chickens

et al. 2022

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“…Proteolysis causes the collapse of the muscle architecture, thus reducing the force generation ability and the force transmission capacity along and across the muscle fiber. As a result, muscles become weak and prone to damage. , Desmin associated atrophy can be explained with two ubiquitin ligases, such as ubiquitin tripartite motif-containing protein 32 (TRIM32) and MURF1. MURF1 catalyzes the breakdown of the thick myosin filament’s proteins, whereas TRIM32 catalyzes the Z-band and the desmin cytoskeleton’s breakdown.…”

Section: Sarcopenia Markersmentioning

confidence: 99%

Molecular Mechanisms and the Interplay of Important Chronic Obstructive Pulmonary Disease Biomarkers Reveals Novel Therapeutic Targets

Sharma,

Banerjee,

Srivastava

2023

ACS Omega

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Chronic Obstructive Pulmonary Disease (COPD) is a progressive, age-dependent, and unmet chronic inflammatory disease of the peripheral airways, leading to difficulty in exhalation. Several biomarkers have been tested in general towards the resolution for a long time, but no apparent success was achieved. Ongoing therapies of COPD have only symptomatic relief but no cure. Reactive oxygen species (ROS) are highly reactive species which include oxygen radicals and nonradical derivatives, and are the prominent players in COPD. They are produced as natural byproducts of cellular metabolism, but their levels can vary due to exposure to indoor air pollution, occupational pollution, and environmental pollutants such as cigarette smoke. In COPD, the lungs are continuously exposed to high levels of ROS thus leading to oxidative stress. ROS can cause damage to cells, proteins, lipids, and DNA which further contributes to the chronic inflammation in COPD and exacerbates the disease condition. Excessive ROS production can overwhelm cellular antioxidant systems and act as signaling molecules that regulate cellular processes, including antioxidant defense mechanisms involving glutathione and sirtuins which further leads to cellular apoptosis, cellular senescence, inflammation, and sarcopenia. In this review paper, we focused on COPD from different perspectives including potential markers and different cellular processes such as apoptosis, cellular senescence, inflammation, sirtuins, and sarcopenia, and tried to connect the dots between them so that novel therapeutic strategies to evaluate and target the possible underlying mechanisms in COPD could be explored.

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“…It reduces sarcomere tension range, leading to muscular passive stiffness. The mechanical characteristics of striated muscle (cardiac or skeletal) vary with titin sequence [15]. Titin's main roles are to maintain the thick filament, centre it between the thin filaments, avoid overstretching, and rebound the sarcomere like a spring [16].…”

Section: Introductionmentioning

confidence: 99%

The assessment of Dystrophin and Titin in premenopausal thyroid women patients

Mahmod¹,

Jaffat²

2023

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The aim, this study was designed to investigate the relationships between Dystrophin and Titin in patients with thyroid dysfunction. Materials and methods. The study included 90 patients; aged between 15 and 45 years, divided into three groups, 60 patients with thyriodism and 30 healthy subjects were taken as control group, all patients (n=60) were divided into two groups hypothyroidism and hyperthyroidism. This cross-sectional study was carried out in Al-Fadel foundation for training and development (research foundation) in Babylon province. Using 5 ml disposable syringes, healthy controls and patients were randomly punctured for blood samples. Blood was placed in a centrifuge tube and allowed to coagulate for 15 minutes before being spun for 10 minutes at 1000 xg to 2000 xg to separate the serum for immunological testing. Results. The Mean Serum Titin levels which indicated significant differences in studied groups of hypothyroidism, and hyperthyroidism patients, and the control groups. Remark that Titin levels decreased in Hypothyroidism and hyperthyroidism groups less than in the healthy control group. The statistical analysis results of Serum Dystrophin levels found significant differences in the three groups studied. These results revealed a significant decline in hypothyroidism patients, and hyperthyroidism patients, compared with the healthy control group, while Dystrophin level was increased in hypothyroidism more than in the hyperthyroidism patients. Conclusion. The results of T3, T4 &TSH level showed significant differences after the comparison between hyperthyroidism, hypothyroidism and control. There are significant differences in the levels of DMD and TTN in comparison between Hyperthyroidism, and Hypothyroidism as well as with control.

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Sarcomere Structure: The Importance of Desmin Protein in Muscle Atrophy

Cited by 10 publications

References 47 publications

Differential expression patterns of genes associated with metabolisms, muscle growth and repair in Pectoralis major muscles of fast- and medium-growing chickens

Differential expression patterns of genes associated with metabolisms, muscle growth and repair in Pectoralis major muscles of fast- and medium-growing chickens

Molecular Mechanisms and the Interplay of Important Chronic Obstructive Pulmonary Disease Biomarkers Reveals Novel Therapeutic Targets

The assessment of Dystrophin and Titin in premenopausal thyroid women patients

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