Sarothralen A and B, New Antibiotic Compounds from<i>Hypericum japonicum</i>

Ishiguro, Kyoko; Yamaki, Masae; Kashihara, Mikiko; Takagi, Shûzô

doi:10.1055/s-2007-969154

Cited by 50 publications

(29 citation statements)

References 2 publications

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“…(Chinese name, Tianjihuang) has been used as a Chinese medicinal herb for the treatment of infectious hepatitis, bacterial diseases and tumors for many years (Hu et al, 1996;Ishiguro et al, 1986;Wu et al, 1998;Li et al, 1993). Previous phytochemical studies of Tianjihuang have demonstrated that it mainly contains flavonoids (Wu et al, 1998;Ishiguro et al, 1991bIshiguro et al, , 1993, phloroglucinol derivatives (Hu et al, 2000), xanthonoids (Ishiguro et al, 1997), chromone glycosides (Wu et al, 1998;Ishiguro et al, 1990) and peptides (Ishiguro et al, 1991a).…”

Section: Introductionmentioning

confidence: 99%

HPLC analysis and pharmacokinetic study of quercitrin and isoquercitrin in rat plasma after administration of Hypericum japonicum thunb. extract

Wang

Zhang

et al. 2007

Biomedical Chromatography

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A simple HPLC method was developed for determination of quercitrin and isoquercitrin in rat plasma. Reversed-phase HPLC was employed for the quantitative analysis using kaempferol-3-O-beta-D-glucopyranoside-7-O-alpha-L-rhamnoside as an internal standard. Following extraction from the plasma samples with ethyl acetate-isopropanol (95:5, v/v), these two compounds were successfully separated on a Luna C(18) column (250 x 4.6 mm, 5 microm) with isocratic elution of acetonitrile-0.5% aqueous acetic acid (17:83, v/v) as the mobile phase. The flow-rate was set at 1 mL/min and the eluent was detected at 350 nm for both quercitrin and isoquercitrin. The method was linear over the studied ranges of 50-6000 and 50-5000 ng/mL for quercitrin and isoquercitrin, respectively. The intra- and inter-day precisions of the analysis were better than 13.1 and 13.2%, respectively. The lower limits of quantitation for quercitrin and isoquercitrin in plasma were both of 50 ng/mL. The mean extraction recoveries were 73 and 61% for quercitrin and isoquercitrin, respectively. The validated method was successfully applied to pharmacokinetic studies of the two analytes in rat plasma after the oral administration of Hypericum japonicum thunb. ethanol extract.

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Section: Introductionmentioning

confidence: 99%

HPLC analysis and pharmacokinetic study of quercitrin and isoquercitrin in rat plasma after administration of Hypericum japonicum thunb. extract

Wang

Zhang

et al. 2007

Biomedical Chromatography

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“…HJT, an annual herb from the genus Hypericum L., known in traditional Chinese medicine as Tian-ji-huang, grows mainly throughout southern China. HJT has been used in a Chinese herbal compound formula for many years for the treatment of bacterial diseases; infectious, acute and chronic hepatitis; gastrointestinal disorder; internal hemorrhage and tumors (13,14). Data in the present study demonstrated that the HJT extract significantly inhibited the growth of H22-transplanted tumors and had a synergistic tumor-inhibiting effect with the 5-FU group.…”

Section: Discussionmentioning

confidence: 47%

The effect-enhancing and toxicity-reducing activity of Hypericum japonicum Thunb. extract in murine liver cancer chemotherapy

Lü

Cao

Zhen-hua

et al. 2012

Molecular and Clinical Oncology

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Abstract. Chinese herbs are potential sources of antitumor drugs with immunoregulatory activity and few adverse effects. In the present study, we investigated whether the Hypericum japonicum Thunb. (HJT) extract enhanced the efficacy of 5-fluorouracil (5-FU) treatment in murine liver tumor xenografts and reduced toxicity of chemotherapy in hepatoma H22-bearing mice. Tumor weight and inhibition rate, thymus and spleen indices, as well as white blood cell (WBC) count were calculated. The phagocytic function of macrophages was assessed by observing peritoneal macrophages phagocytized chicken red blood cells (RBC). Body weight and toxic reactions of the chemotherapeutic and life prolongation rate were investigated in the mice. Results demonstrated that the HJT extract significantly enhanced the tumor inhibition rate of 5-FU, improved the immune function, reduced the toxic effects and prolonged the survival time in the tumor-bearing mice. Taken together, these results indicated that the HJT extract has a synergistic tumor-inhibiting effect with 5-FU, is able to reduce the toxic side effects and is likely to be safe and efficacious for use in antitumor therapy. IntrodutionLiver cancer is a malignant disease with a high incidence and mortality, and is often diagnosed at an advanced stage (1). Common therapies, such as surgical resection, percutaneous or transarterial interventions are of limited efficacy (2), as chemotherapy, one of the main measures for a comprehensive treatment, may induce adverse effects such as bone marrow suppression, gastrointestinal reactions and lowered immunological function.Several Chinese herbs have been identified as potential sources of antitumor drugs (3-5). The effect-enhancing and toxicity-reducing activity of Chinese herbs in radiotherapy and chemotherapy of tumors was previously confirmed (6,7). However, no studies focusing on the antitumor and toxicity-reducing effects of Hypericum japonicum Thunb. (HJT) have been reported thus far. Therefore, the present investigation was conducted to ascertain whether the HJT extract enhanced the efficacy of 5-fluorouracil (5-FU) treatment in murine liver tumor xenografts, and reduced toxicity of chemotherapy in tumor-bearing mice. Materials and methodsReagents. RPMI-1640 medium, fetal bovine serum (FBS), penicillin G and streptomycin were obtained from Invitrogen Corp. (Carlsbad, CA, USA). 5-FU was obtained from the Shanghai Xudong Haipu Pharmaceutical Co., Ltd. (Shanghai, China). Murine H22 hepatoma cells were purchased from the Shanghai Institute of Cell Biology at the Chinese Academy of Sciences (Shanghai, China). Animals. Institute of Cancer Research (ICR) mice(male/female, 50-50%; weight, 20±2 g) were purchased from the Experimental Animal Center, Zhengzhou University (Zhengzhou, China). The animals were kept in a standard laboratory, under a 12-h light/dark cycle, at room temperature (20±2˚C), in a humid (75±15%) and filtered laminar air flow-controlled room in the animal facility of the Experimental Animal Center of the Zhengzhou University...

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“…H. perforatum also displayed anti-immobility effect in the forced swimming test (Butterweck et al, 1997(Butterweck et al, , 1998. The most lipophilic fraction of several species of the genus Hypericum is rich in phloroglucinol derivatives (Ishiguro et al, 1986;Jayasuriya et al, 1991;Rocha et al, 1994Rocha et al, , 1995. Traditionally, the naphthodianthrone, hypericin, was considered to be the most active constituent of H. perforatum, but only few data are still in favour of the hypothesis that the antidepressant activity is due to hypericin only (Wagner and Bladt, 1994;Butterweck et al, 1998).…”

Section: Resultsmentioning

confidence: 99%

Screening for the antidepressant activity of some species ofHypericum from South Brazil

et al. 2000

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Sarothralen A and B, New Antibiotic Compounds fromHypericum japonicum

Abstract: Two antibiotic compounds, sarothralen A and sarothralen B, from Hypericum japonicum Thunb. ( Sarothra japonica Thunb.) have been isolated and their structures were elucidated on the basis of spectroscopic data. The compounds contain phloroglucinol and filicinic acid residues.

Cited by 50 publications

References 2 publications

HPLC analysis and pharmacokinetic study of quercitrin and isoquercitrin in rat plasma after administration of Hypericum japonicum thunb. extract

HPLC analysis and pharmacokinetic study of quercitrin and isoquercitrin in rat plasma after administration of Hypericum japonicum thunb. extract

The effect-enhancing and toxicity-reducing activity of Hypericum japonicum Thunb. extract in murine liver cancer chemotherapy

Screening for the antidepressant activity of some species ofHypericum from South Brazil

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