SARS-CoV-2-fehérjék kimutatása immunhisztokémiai módszerrel emberi szövetekben.

Pesti, Adrián; Gyömörei, Csaba; Juhász, Péter; Kálmán, Endre; Kiss, András; Kuthi, Levente; Lotz, G.; Méhes, Gábor; Schaff, Zsuzsa; Tiszlavicz, László

doi:10.1556/650.2022.32536

Cited by 4 publications

(2 citation statements)

References 41 publications

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“…While RT-PCR remains the gold standard for SARS-CoV-2 diagnosis, immunohistochemistry has emerged as a valuable tool in the detection of SARS-CoV-2, showcasing notable efficiency than other alternative methods. Immunohistochemistry provides valuable spatial information, visualizing viral antigen localization within infected cells, which can aid in understanding the viral distribution and potential tissue preference, as previously reported by Pesti et al [45,46]. However, IHC requires specialized expertise for its interpretation, potentially limiting its widespread application.…”

Section: Immunohistochemistry As a Methods Of Virus Detectionmentioning

confidence: 95%

Immunohistochemical and Morphometric Analysis of Lung Tissue in Fatal COVID-19

Gheban-Roșca,

Gheban,

Pop

et al. 2024

Diagnostics

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The primary targets of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the lungs are type I pneumocytes, macrophages, and endothelial cells. We aimed to identify lung cells targeted by SARS-CoV-2 using viral nucleocapsid protein staining and morphometric features on patients with fatal COVID-19. We conducted a retrospective analysis of fifty-one autopsy cases of individuals who tested positive for SARS-CoV-2. Demographic and clinical information were collected from forensic reports, and lung tissue was examined for microscopic lesions and the presence of specific cell types. Half of the evaluated cohort were older than 71 years, and the majority were male (74.5%). In total, 24 patients presented diffuse alveolar damage (DAD), and 50.9% had comorbidities (56.9% obesity, 33.3% hypertension, 15.7% diabetes mellitus). Immunohistochemical analysis showed a similar pattern of infected macrophages, infected type I pneumocytes, and endothelial cells, regardless of the presence of DAD (p > 0.5). The immunohistochemical reactivity score (IRS) was predominantly moderate but without significant differences between patients with and without DAD (p = 0.633 IRS for type I pneumocytes, p = 0.773 IRS for macrophage, and p = 0.737 for IRS endothelium). The nucleus/cytoplasm ratio shows lower values in patients with DAD (median: 0.29 vs. 0.35), but the difference only reaches a tendency for statistical significance (p = 0.083). Our study confirms the presence of infected macrophages, type I pneumocytes, and endothelial cells with a similar pattern in patients with and without diffuse alveolar damage.

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Section: Immunohistochemistry As a Methods Of Virus Detectionmentioning

confidence: 95%

Immunohistochemical and Morphometric Analysis of Lung Tissue in Fatal COVID-19

Gheban-Roșca,

Gheban,

Pop

et al. 2024

Diagnostics

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“…Az ileum és a colon enterocytáiban nagy számban expresszálódik mind az ACE2-, mind a TMPRSS2-receptor, így a vírus nagyon könnyen be tud jutni az enterocytákba [10,12,13]. A SARS-CoV-2-RNS a COVID-19-betegek nagy százalékában kimutatható a székletből [10][11][12]14]. Szignifikáns összefüggés azonban nem igazolódott a vírus székletben történő kimutathatósága és a vírusfertőzés során jelentkező gastrointestinalis tünetek súlyossága között [10,15,16].…”

Section: Esetismertetésunclassified

Idegsebészeti posztoperatív őrzőben kezelt idős betegek SARS-CoV-2 és Clostridioides difficile kombinált fertőzése

Oláh,

Czakó,

Godó

et al. 2024

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A COVID–19-pandémia során fellépő Clostridioides difficile fertőzés incidenciájával kapcsolatban egymással ellentétes eredmények ismertek: számos tanulmány szerint növekedett, míg más tanulmányok szerint csökkent. A második COVID–19-hullám tetőfokán idegsebészeti posztoperatív őrzőnkben 11, intenzív ellátást igénylő beteget kezeltünk. A 11 betegből 7 COVID–19-pozitív lett, és közülük 4 betegnél még C. difficile fertőzés is kialakult. A 4-ből 2 beteget elveszítettünk. Bár megfigyeléseinket kevés beteg ellátása során gyűjtöttük, úgy ítéljük meg, hogy súlyos állapotú betegeknél a SARS-CoV-2 és a C. difficile együttes fertőzése többletkockázatot jelent. A COVID–19-ben alkalmazott, széles spektrumú szisztémás antibiotikumok használata növelheti a C. difficile infekció rizikóját. Jól átgondolt infekciókontroll, a megfelelő higiéné betartása, az alkoholos kézfertőtlenítők mellett a rendszeres szappanos kézmosások mérsékelhetik a nosocomialis C. difficile fertőzések számát. Orv Hetil. 2024; 165(12): 464–469.

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Liver alterations and detection of SARS-CoV-2 RNA and proteins in COVID-19 autopsies

et al. 2022

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The most severe alterations in Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) infection are seen in the lung. However, other organs also are affected. Here, we report histopathologic findings in the liver and detection of viral proteins and RNA in COVID-19 autopsies performed at the Semmelweis University (Budapest, Hungary). Between March 2020 through March 2022, 150 autopsies on patients who died of COVID-19 were analyzed. Cause-of-death categories were formed based on the association with SARS-CoV-2 as strong, contributive, or weak. Samples for histopathologic study were obtained from all organs, fixed in formalin, and embedded in paraffin (FFPE). Immunohistochemical study (IHC) to detect SARS-CoV-2 spike protein and nucleocapsid protein (NP), CD31, claudin-5, factor VIII, macrosialin (CD68), and cytokeratin 7, with reverse transcriptase polymerase chain reaction (RT-PCR), and in situ hybridization (ISH, RNAscope®) for SARS-CoV-2 RNA were conducted using FFPE samples of livers taken from 20 autopsies performed ≤ 2 days postmortem. All glass slides were scanned; the digital images were evaluated by semiquantitative scoring and scores were analyzed statistically. Steatosis, single-cell and focal/zonal hepatocyte necrosis, portal fibrosis, and chronic inflammation were found in varying percentages. Sinusoidal ectasia, endothelial cell disruption, and fibrin-filled sinusoids were seen in all cases; these were assessed semiquantitatively for severity (SEF scored). SEF scores did not correlate with cause-of-death categories (p = 0.92) or with severity of lung alterations (p = 0.96). SARS-CoV-2 RNA was detected in 13/20 cases by PCR and in 9/20 by ISH, with IHC demonstration of spike protein in 4/20 cases and NP in 15/20. Viral RNA and proteins were located in endothelial and Kupffer cells, and in portal macrophages, but not in hepatocytes and cholangiocytes. In conclusion, endothelial damage (SEF scores) was the most common alteration in the liver and was a characteristic, but not specific alteration in COVID-19, suggesting an important role in the pathogenesis of COVID-19-associated liver disease. Detection of SARS-CoV-2 RNA and viral proteins in liver non-parenchymal cells suggests that while the most extended primary viral cytotoxic effect occurs in the lung, viral components are present in other organs too, as in the liver. The necrosis/apoptosis and endothelial damage associated with viral infection in COVID-19 suggest that those patients who survive more severe COVID-19 may face prolonged liver repair and accordingly should be followed regularly in the post-COVID period.

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SARS-CoV-2-fehérjék kimutatása immunhisztokémiai módszerrel emberi szövetekben.

Cited by 4 publications

References 41 publications

Immunohistochemical and Morphometric Analysis of Lung Tissue in Fatal COVID-19

Immunohistochemical and Morphometric Analysis of Lung Tissue in Fatal COVID-19

Idegsebészeti posztoperatív őrzőben kezelt idős betegek SARS-CoV-2 és Clostridioides difficile kombinált fertőzése

Liver alterations and detection of SARS-CoV-2 RNA and proteins in COVID-19 autopsies

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