ObjectiveThis study aims to determine the role of personal protective measures in the prevention of COVID-19 spread among the physicians working at different health facilities in Bangladesh.MethodsThis hospital-based cross-sectional comparative was conducted from May to June 2020. A total of 98 COVID-19 positive physicians and 92 COVID-19 negative physicians (physicians having no symptoms of COVID-19 or tested negative), were enrolled. The questionnaire was adapted from a tool developed by the World Health Organization (WHO) for risk assessment and management of exposure of health care workers in the context of COVID-19. Data was collected from the respondents online using google forms.ResultsThere was no significant difference in baseline information between COVID-19 positive and COVID-19 negative physicians. The physicians, who were unaware of direct participation in COVID-19 patient care, had higher odds of being COVID-19 positive (OR= 4.018; CI: 1.532-10.535). Also, the physicians, who were unaware of the COVID-19 status while performing aerosol generated procedure (AGP), had a higher chance of being COVID-19 positive, had a higher chance of being COVID-19 positive (OR= 2.522; CI: 1.020-6.233). Using face shields/goggles (OR=0.437; CI:0.228-0.837), regular decontamination of the patient’s surroundings (OR=0.392; CI:0.176-0.873) while usual take care of patients and use of N95 masks while performing AGP (OR=0.372; CI:0.159-0.873) had protective roles against COVID-19 among the physicians. The physicians who had reused the medical gown had two times more chance of being tested positive for COVID-19 than those who had not reused it (OR= 2.3; CI:1.251-4.259).ConclusionThe use of face shield/goggles and N95 masks and decontamination of the patient’s surroundings may give protection against COVID-19. Also, reusing medical gown should be avoided as much as possible.