2021
DOI: 10.3390/microorganisms9081744
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SARS-CoV-2 Spike Pseudoviruses: A Useful Tool to Study Virus Entry and Address Emerging Neutralization Escape Phenotypes

Abstract: SARS-CoV-2 genetic variants are emerging around the globe. Unfortunately, several SARS-CoV-2 variants, especially variants of concern (VOCs), are less susceptible to neutralization by the convalescent and post-vaccination sera, raising concerns of increased disease transmissibility and severity. Recent data suggests that SARS-CoV-2 neutralizing antibody levels are a reliable correlate of vaccine-mediated protection. However, currently used BSL3-based virus micro-neutralization (MN) assays are more laborious, t… Show more

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Cited by 21 publications
(13 citation statements)
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“…We also tested S from circulating variants and observed robust MM43-mediated neutralization of SC2-VLPs generated from Alpha, Beta, Gamma, B.1.617.2 (Delta), and Epsilon variant S proteins (Fig. 3D) and consistent with previous studies ( 29 ). These results show that SC2-VLPs employ S-mediated entry and can be used for screening S mutations for entry and neutralization.…”
supporting
confidence: 89%
“…We also tested S from circulating variants and observed robust MM43-mediated neutralization of SC2-VLPs generated from Alpha, Beta, Gamma, B.1.617.2 (Delta), and Epsilon variant S proteins (Fig. 3D) and consistent with previous studies ( 29 ). These results show that SC2-VLPs employ S-mediated entry and can be used for screening S mutations for entry and neutralization.…”
supporting
confidence: 89%
“…Pseudovirus neutralization is a standard assay to detect the presence and magnitude of functional antibodies or binders that can prevent infectivity of a virus, e.g. SARS-CoV-2 ( Kalkeri et al, 2021 ). Therefore, to cross validate the binding data, we further examined SI–F019 potency by the pseudovirus neutralization assay.…”
Section: Resultsmentioning
confidence: 99%
“…Although the pseudovirus system facilitates S protein studies, some concerns remain. In the pseudovirus-based experiment, cell lines express exogenous human ACE2 only or co-express transmembrane Serine Protease 2 (TMPRSS2) to facilitate the pseudovirus infectivity [ 29 , 30 ]. The S protein density in the viral surface may influence cell entry and neutralization ability [ 31 , 32 ].…”
Section: Application Of Pseudovirus In Sars-cov-2 Neutralization Assaymentioning
confidence: 99%