SARS-CoV-2 surveillance in Norway rats (<i>Rattus norvegicus</i>) from Antwerp sewer system, Belgium

Colombo, Valeria; Sluydts, Vincent; Mariën, Joachim; Broecke, Bram Vanden; Houtte, Natalie Van; Leirs, Wannes; Jacobs, Lotte; Iserbyt, Arne; Hubert, Marine; Heyndríckx, Leo; Goris, Hanne; Delputte, Peter; Roeck, Naomi De; Elst, Joris; Boudewijns, Robbert; Ariën, Kevin K.; Leirs, Herwig; Gryseels, Sophie

doi:10.1101/2021.03.06.433708

Cited by 3 publications

(1 citation statement)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accordingly, field studies were initiated early into the pandemic to investigate the situation in wild rats. Indeed, serological and molecular evidences of SARS-CoV-2 infection of a few animals could be found in some studies (2,3,5), while others reported consistently negative results (6,7). However, these studies were conducted before the emergence and worldwide large-scale spread of the omicron VOC and its diverse subvariants.…”

Section: Main Textmentioning

confidence: 99%

SARS-CoV-2 and other coronaviruses in rats, Berlin, Germany, 2023

Wernike,

Mehl,

Aebischer

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Section: Main Textmentioning

confidence: 99%

SARS-CoV-2 and other coronaviruses in rats, Berlin, Germany, 2023

Wernike,

Mehl,

Aebischer

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Biodistribution and Cellular Internalization of Inactivated SARS-CoV-2 in Wild-Type Mice

Hudák¹,

Morgan²,

Bačovský

et al. 2022

IJMS

View full text Add to dashboard Cite

Despite the growing list of identified SARS-CoV-2 receptors, the human angiotensin-converting enzyme 2 (ACE2) is still viewed as the main cell entry receptor mediating SARS-CoV-2 internalization. It has been reported that wild-type mice, like other rodent species of the Muridae family, cannot be infected with SARS-CoV-2 due to differences in their ACE2 receptors. On the other hand, the consensus heparin-binding motif of SARS-CoV-2′s spike protein, PRRAR, enables the attachment to rodent heparan sulfate proteoglycans (HSPGs), including syndecans, a transmembrane HSPG family with a well-established role in clathrin- and caveolin-independent endocytosis. As mammalian syndecans possess a relatively conserved structure, we analyzed the cellular uptake of inactivated SARS-CoV-2 particles in in vitro and in vivo mice models. Cellular studies revealed efficient uptake into murine cell lines with established syndecan-4 expression. After intravenous administration, inactivated SARS-CoV-2 was taken up by several organs in vivo and could also be detected in the brain. Internalized by various tissues, inactivated SARS-CoV-2 raised tissue TNF-α levels, especially in the heart, reflecting the onset of inflammation. Our studies on in vitro and in vivo mice models thus shed light on unknown details of SARS-CoV-2 internalization and help broaden the understanding of the molecular interactions of SARS-CoV-2.

show abstract

Monitoring Urban Zoonotic Virus Activity: Are City Rats a Promising Surveillance Tool for Emerging Viruses?

Camp

Desvars-Larrive

Nowotny

et al. 2022

Viruses

View full text Add to dashboard Cite

Urban environments represent unique ecosystems where dense human populations may come into contact with wildlife species, some of which are established or potential reservoirs for zoonotic pathogens that cause human diseases. Finding practical ways to monitor the presence and/or abundance of zoonotic pathogens is important to estimate the risk of spillover to humans in cities. As brown rats (Rattus norvegicus) are ubiquitous in urban habitats, and are hosts of several zoonotic viruses, we conducted longitudinal sampling of brown rats in Vienna, Austria, a large population center in Central Europe. We investigated rat tissues for the presence of several zoonotic viruses, including flaviviruses, hantaviruses, coronaviruses, poxviruses, hepatitis E virus, encephalomyocarditis virus, and influenza A virus. Although we found no evidence of active infections (all were negative for viral nucleic acids) among 96 rats captured between 2016 and 2018, our study supports the findings of others, suggesting that monitoring urban rats may be an efficient way to estimate the activity of zoonotic viruses in urban environments.

show abstract

SARS-CoV-2 surveillance in Norway rats (Rattus norvegicus) from Antwerp sewer system, Belgium

Cited by 3 publications

References 45 publications

SARS-CoV-2 and other coronaviruses in rats, Berlin, Germany, 2023

SARS-CoV-2 and other coronaviruses in rats, Berlin, Germany, 2023

Biodistribution and Cellular Internalization of Inactivated SARS-CoV-2 in Wild-Type Mice

Monitoring Urban Zoonotic Virus Activity: Are City Rats a Promising Surveillance Tool for Emerging Viruses?

Contact Info

Product

Resources

About