New data were obtained for the Chulym River basin in the southeastern part of the West Siberian Plain, one of the understudied parts of Siberia in terms of age and composition of carbon and nitrogen stable isotopes for Late Pleistocene megafauna. The 14C dates from the Sergeevo outcrop, the most complete section of Late Quaternary deposits in the region, are mostly greater than ~30 550 bp. Other localities yielded 14C values in the range from >44 500 to ~19 300 bp. The finite date of ~42 270 bp for the Khozarian steppe elephant (Mammuthus trogontherii chosaricus) from Asino is intriguing because previously it was not detected in the Late Pleistocene of Siberia after the last interglacial (Marine Isotope Stage 5e), ~115 000–130 000 years ago. Stable isotope data show both similarities and differences compared to the pre‐Last Glacial Maximum megafaunal species in other parts of Siberia.