SATB1 promotion of trophoblast stem cell renewal through regulation of threonine dehydrogenase

Kubota, Kaiyu; Iqbal, Khursheed; Soares, Michael J.

doi:10.1016/j.bbagen.2020.129757

Cited by 2 publications

(3 citation statements)

References 47 publications

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“…At EA 18, we found that lentivirus containing ATP6AP2 shRNA reduced placental labyrinth ATP6AP2 mRNA expression by 52.4% and protein levels by 48.3% but had no effect on the expression of (P)RR in the junctional zone (Figures 4B, C). Our study therefore produced a similar degree of gene knockdown as other studies utilising this method with 70%-80% (Kubota et al, 2021;Muto et al, 2021) and 40%-60% (Wang et al, 2021) reductions in their genes of interest.…”

Section: Discussionmentioning

confidence: 66%

Placental deficiency of the (pro)renin receptor ((P)RR) reduces placental development and functional capacity

Schofield,

Kahl,

Rodrigues

et al. 2023

Front. Cell Dev. Biol.

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The (pro)renin receptor ((P)RR; also known as ATP6AP2) is a multifunctional receptor. The (P)RR activates the tissue renin-angiotensin system (RAS) and is also involved in regulating integral intracellular pathways such as V-ATPase and Wnt/β-catenin signalling. Given this, the (P)RR may be associated with essential pathways in placentation, however its role within the context of pregnancy remains poorly characterised. The first trimester/extravillous trophoblast cell line, HTR-8/SVneo, underwent an siRNA knockdown where they were incubated for 24 h with a negative control siRNA or siRNA targeting ATP6AP2 mRNA. xCELLigence real-time cell analysis was performed to assess the effect of ATP6AP2 mRNA knockdown on HTR-8/SVneo cell proliferation, migration, and invasion. In subsequent experiments, GFP-encoding lentiviral packaged gene-constructs were used to knockdown (P)RR expression in the trophectoderm of C57/BL6/CBA-F1 mouse blastocysts. Blastocysts were incubated for 6 h with vehicle (no-virus), control virus (non-targeting shRNA and GFP), or (P)RR-knockdown virus ((P)RR shRNA and GFP) before transfer into recipient pseudo-pregnant Swiss CD1 female mice. Fetal and placental tissues were collected and assessed at embryonic age (EA) 10 and 18. (P)RR levels were measured in the labyrinth zone of day 18 placentae and stereological Merz grid analysis was performed to determine the volumetric distribution of trophoblasts, fetal capillaries, and the maternal blood space. We showed that a reduction of ATP6AP2 expression in HTR-8/SVneo cells in vitro, impaired trophoblast proliferation, migration, and invasion. In vivo, decreasing placental labyrinth (P)RR expression adversely effected placental physiology, decreasing placental trophoblast number and total surface area available for exchange, while also increasing maternal blood space. Additionally, decreased (P)RR affected placental efficacy evident by the reduced fetal-placental weight ratio. Our study shows that the (P)RR is necessary for appropriate placental development and function.

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Section: Discussionmentioning

confidence: 66%

Placental deficiency of the (pro)renin receptor ((P)RR) reduces placental development and functional capacity

Schofield,

Kahl,

Rodrigues

et al. 2023

Front. Cell Dev. Biol.

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“…The self-renewal of stem cells is highly dependent on AA metabolism, including threonine metabolism, One-C metabolism, methionine cycle, glutamine metabolism, and L-Pro metabolism, indicating that AA metabolism is essential for the growth of stem cells (Figure 2) [51,52]. It is worth noting that mESCs mainly rely on the breakdown metabolism of threonine, while hESCs achieve selfrenewal through the breakdown metabolism of methionine [53,54]. We summarize the regulation of stem cell pluripotency, self-renewal, and somatic reprogramming by AA metabolism, and these research findings reveal the close relationship between AA metabolism and stem cells.…”

Section: Amino Acid (Aa) Metabolism Regulates Stem Cell Functionmentioning

confidence: 99%

“…Protein arginine methyltransferase (PRMT5) plays an important role in regulating DNA repair, cell cycle, and transcriptional regulation processes, improving reprogramming efficiency by positively regulating TDH expression [57]. SATB homeobox 1 (SATB1) is a tissue-specific nuclear matrix binding protein that promotes trophoblast stem cell renewal by regulating the expression of TDH in trophoblast stem cells [53]. The driving protein binding protein (KBP) plays an important role in neural development.…”

Section: Threonine Metabolismmentioning

confidence: 99%

Energy Metabolism Pathways in Stem Cell Fate Regulation

Wang,

Yang,

Cao

2024

Preprint

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Energy metabolism regulates the proliferation of stem cells by regulating energy production and the production of substrates through biosynthetic pathways. It is an important target for cell regulation and stem cell applications, and is crucial for determining the pluripotency and cell fate of stem cells. In this context, this article focuses on reviewing the latest research progress on the association between glucose metabolism, amino acid metabolism, fatty acid metabolism, and mitophagy with stem cell function, elucidating how key energy metabolism pathways affect stem cell function by regulating signal transduction and epigenetic modifications. In this review, we discuss the key factors affecting energy metabolism and their association with stem cell function. The integration of energy metabolism and stem cell fate regulation will open up a new path for future regenerative medicine practices.

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SATB1 promotion of trophoblast stem cell renewal through regulation of threonine dehydrogenase

Cited by 2 publications

References 47 publications

Placental deficiency of the (pro)renin receptor ((P)RR) reduces placental development and functional capacity

Placental deficiency of the (pro)renin receptor ((P)RR) reduces placental development and functional capacity

Energy Metabolism Pathways in Stem Cell Fate Regulation

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