The purpose of this study is to reveal the seasonal difference in vegetation variation and its seasonal response to climate factors in the Qilian Mountains (QM) under the background of global warming. Based on the MOD13 A2 normalized difference vegetation index (NDVI) data and meteorological data, this study analyzed the spatiotemporal dynamics and stability of vegetation in different seasons by using the mean value method, trend analysis and stability analysis method, and discussed their seasonal responses to climatic factors based on the correlation analysis method. The results show that the vegetation cover in the QM experienced a significant upward trend in the past 21 years, but there were obvious spatial differences in vegetation change in different seasons. The growth rate of vegetation in summer was the fastest, and summer vegetation provided the most significant contribution to the growing season vegetation. The order of vegetation stability in the QM among the seasons was growing season > summer > spring > autumn. The vegetation change was obviously affected by temperature in spring, while it was mainly controlled by precipitation in the growing season and summer. The response of vegetation to climatic factors was not significant in autumn. Our results can provide important data support for ecological protection in the QM and socioeconomic development in the Hexi Corridor.