Saturable CD14-dependent binding of fluorescein-labeled lipopolysaccharide to human monocytes

Troelstra, Annet; Antal‐Szalmás, Péter; Graaf-Miltenburg, L. A. M. De; Weersink, A. J. L.; Verhoef, J.; Kessel, Kok P. M. van; Strijp, Jos A. G. van

doi:10.1128/iai.65.6.2272-2277.1997

Cited by 55 publications

(31 citation statements)

References 42 publications

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“…The latter hypothesis remains however, unlikely because LBP is not required when LPS is used at high doses. 50 The inability of Hp to act at low doses may reflect its neutral role in the immune system under normal physiological conditions. However, the initiation of an inflammatory process will increase Hp synthesis by the liver, and Hp might then exhibit its anti-inflammatory potential to slow down the inflammation.…”

Section: Discussionmentioning

confidence: 99%

Haptoglobin dampens endotoxin‐induced inflammatory effects both in vitro and in vivo

Kasran²,

et al. 2005

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Summary We report that haptoglobin, an acute‐phase protein produced by liver cells in response to interleukin‐6 (IL‐6), can modulate the inflammatory response induced by endotoxins. We provide evidence that haptoglobin has the ability to selectively antagonize lipopolysaccharide (LPS) effects in vitro by suppressing monocyte production of tumour necrosis factor‐α, IL‐10 and IL‐12, while it fails to inhibit the production of IL‐6, IL‐8 and IL‐1 receptor antagonist. In two animal models of LPS‐induced bronchopulmonary hyperreactivity and endotoxic shock, haptoglobin knockout mice were more sensitive to LPS effects compared to their wild‐type counterparts. The present data suggest that haptoglobin regulates monocyte activation following LPS stimulation. The increase in haptoglobin levels during an acute‐phase reaction may generate a feedback effect which dampens the severity of cytokine release and protects against endotoxin‐induced effects.

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Section: Discussionmentioning

confidence: 99%

Haptoglobin dampens endotoxin‐induced inflammatory effects both in vitro and in vivo

Kasran²,

et al. 2005

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“…The binding characteristics of LPS to CD14 were studied by several authors, defining the CD14-dependent binding with blocking anti-CD14 mAbs or comparing binding in the presence and absence of excess LBP or serum [52,53,62,63]. CD14-mediated binding of E. coli K12 LCD25 Ra or Rb LPS to CD14-transfected CHO cells or to differentiated THP-1 cells showed saturation at 100-500 ng mL ¹1 (about 25 nM) endotoxin with a binding affinity of K D ¼ 10-50 nM and with a CD14 to LPS binding ratio of 1-2, 10-20 or 15 LPS molecules per CD14 [31,53,55,[62][63][64]. According to the most recent model, CD14 forms a ternary complex with LPS and LBP, and not only monomeric but multiple LPS-LBP complexes might bind to CD14.…”

Section: Mcd14 Is the Lps 'Receptor'mentioning

confidence: 99%

Evaluation of CD14 in host defence

Antal‐Szalmás

2000

Eur J Clin Investigation

130

126

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An extensive search for the cell membrane targets for lipopolysaccharide (LPS), the major causative agent of Gram-negative septic shock, resulted in the identification of CD14 as the major endotoxin 'receptor'. Besides recognition of LPS, several new aspects of its biological functions have been described recently. In this review the different CD14 forms, their most important biological and biochemical features, signalling properties, cellular and subcellular distribution and association with different diseases are discussed in detail, showing that these molecules posses several unique biological functions and further proving their central role in innate immunity.

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“…To study the internalization rate and mechanisms of CD14-bound endotoxin on human monocytes, 10 ng/ml FITC-LPS was incubated with 1% NHS and 10 6 mononu-clear cells for 10 min at 37°C. These conditions ensure a totally CD14-dependent binding of LPS to the monocytes (32). The nonbound FITC-LPS was removed and the cells were further incubated for 0, 5, 10, 15, 30, and 60 min to facilitate the internalization of surface-bound endotoxin.…”

Section: Internalization Of Fitc-lps Is Not Accompanied By a Decreasementioning

confidence: 99%

Diverging pathways for lipopolysaccharide and CD14 in human monocytes

Antal‐Szalmás¹,

Poppelier²,

Broekhuizen³

et al. 2000

Cytometry

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Background: CD14 is considered to be the major endo-toxin (lipopolysaccharide [LPS]) binding molecule on human monocytes. It initiates cellular response, but its role in the clearance of LPS is not well understood. Under conditions that ensure totally CD14-dependent LPS binding on human monocytes, the internalization mechanisms of LPS and CD14 were studied. Methods: The uptake and intracellular distribution of fluorescein isothiocyanate (FITC)-LPS and CD14 was determined by flow cytometry, trypan blue quenching, and confocal fluorescence microscopy. Incubation of surface-biotinylated cells with LPS at 37°C or 4°C and subsequent subfractionation was used to further characterize CD14 internalization. The amount of the intracellular CD14 was estimated by CD14 enzyme-linked immunosorbent assay (ELISA). Results: The internalization rate of 10 ng/ml FITC-LPS with 1% human serum was 1% of bound endotoxin per minute, whereas CD14 expression did not decrease at the

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Saturable CD14-dependent binding of fluorescein-labeled lipopolysaccharide to human monocytes

Cited by 55 publications

References 42 publications

Haptoglobin dampens endotoxin‐induced inflammatory effects both in vitro and in vivo

Haptoglobin dampens endotoxin‐induced inflammatory effects both in vitro and in vivo

Evaluation of CD14 in host defence

Diverging pathways for lipopolysaccharide and CD14 in human monocytes

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