Saturated and unsaturated fatty acids differentially regulate in vitro and ex vivo placental antioxidant capacity

Manuel, Clarence R.; Charron, Maureen J.; Ashby, Charles R.; Reznik, Sandra E.

doi:10.1111/aji.12868

Cited by 17 publications

(11 citation statements)

References 28 publications

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“…Further, in Swan71 cells, palmitate decreased cell viability, however oleate (an unsaturated fatty acid) had no effect [50]. The findings from our studies as well as previous studies, suggest that palmitic acid and LA differentially regulate the regulators of cell viability, inflammation and antioxidant capacity [49].…”

Section: Discussionsupporting

confidence: 72%

“…The findings from our study, when compared to work undertaken by others, demonstrates that different fatty acids impact divergent pathways in Swan71 cells. For example, palmitic acid, a saturated fatty acid, has been shown to activate a specific pro-inflammatory response through ROS generation [49], increasing IL-6 secretion [29]. Conversely, we show that LA decreased IL-6 secretion.…”

Section: Discussionmentioning

confidence: 46%

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Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells

Shrestha

Cuffe

Holland

et al. 2019

Cell Physiol Biochem

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 46%

Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells

Shrestha

Cuffe

Holland

et al. 2019

Cell Physiol Biochem

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“…It was previously suggested that PA stimulates lipid accumulation and oxidative stress in normal mouse hepatocytes, as well as stimulating an inflammatory response through the NOX4 and mitochondrial pathways [31]. Moreover, PA also stimulates oxidative stress responses in βTC6 cells [32], HepG2 cells [33] and HTR-8/SVneo cells [34]. Our study revealed that PA treatment promotes the production of MDA, while decreasing the content of GSH, and reducing the activity of CAT, GSH-PX, SOD, and T-AOC in BEND cells.…”

Section: Discussionmentioning

confidence: 99%

Palmitic Acid and β-Hydroxybutyrate Induce Inflammatory Responses in Bovine Endometrial Cells by Activating Oxidative Stress-Mediated NF-κB Signaling

Cai

et al. 2019

Molecules

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Ketosis is a nutritional metabolic disease in dairy cows, and researches indicated that ketonic cows always accompany reproductive problems. When ketosis occurs, the levels of non-esterified fatty acids (NEFAs) and β-hydroxybutyrate (BHBA) in the blood increase significantly. Palmitic acid (PA) is a main component of saturated fatty acids composing NEFA. The aim of this study was to investigate whether high levels of PA and BHBA induce inflammatory responses and regulatory mechanisms in bovine endometrial cells (BEND). Using an enzyme-linked immunosorbent assay, quantitative real-time PCR, and western blotting, we evaluated oxidative stress, pro-inflammatory factors, and the nuclear factor (NF)-κB pathway in cultured BEND cells treated with different concentrations of PA, BHBA, pyrrolidinedithiocarbamate (PDTC, an NF-κB pathway inhibitor), and N-acetylcysteine (NAC, an antioxidant). The content of malondialdehyde was significantly higher, the content of glutathione was lower, and antioxidant activity—glutathione peroxidase, superoxide dismutase, catalase, and total antioxidant capacity—was lower in treated cells compared with control cells. PA- and BHBA-induced oxidative stress activated the NF-κB signaling pathway and upregulated the release of pro-inflammatory factors. Moreover, PA- and BHBA-induced activation of NF-κB-mediated inflammatory responses was inhibited by PDTC and NAC. High concentrations of PA and BHBA induce inflammatory responses in BEND cells by activating oxidative stress-mediated NF-κB signaling.

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“…Possibly, the saturated fatty acids present in this oil could be induced to elevate cell death, reduce total antioxidant capacity, and augment lipid peroxidation. However, it remains unclear how this fatty acid influences collagen behavior (24).…”

Section: Discussionmentioning

confidence: 99%

Effect of a high-fat diet on the rat bladder wall and bioactive action of Brazil nut oil

et al. 2019

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High-fat diet-induced obesity is associated with metabolic disorders. The Brazil nut has bioactive substances and has been used to control the damage caused by obesity in several organs. The work intended to show the damage caused by high-fat diet in the bladder wall and if the Brazil nut oil added to the diet could ameliorate or reverse this effect. Sixty-day-old rats were divided into two groups: C (control, n = 30) and HF (high-fat, n = 30) diets. At 90 days, 10 animals of each group were sacrificed. The others were divided into 4 groups: C and HF (animals that maintained their previous diet, n = 10 for each group) and C / Bno and HF / Bno (animals whose control or high-fat diet was supplemented by Brazil nut oil, n = 10 for each group). Sacrifice occurred at 120 days, and the bladders were removed and analyzed. Epithelial height was increased in the HF compared to the C group. In contrast, the C / Bno had a lower epithelial height compared to the others. The percentage of collagen between the detrusor muscle fibers was significantly greater in C / Bno, HF and HF / Bno than in control group. The HF had a larger muscle fiber diameter than the C group, while the C / Bno presented lower values than the HF and HF / Bno groups. HF diets induced bladder wall damage. These changes in the rat's bladder wall were partially reversed by the Bno.

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Saturated and unsaturated fatty acids differentially regulate in vitro and ex vivo placental antioxidant capacity

Abstract: Our findings suggest that saturated and unsaturated fats differentially regulate placental viability, antioxidant capacity, and inflammation and the actions of gram-positive and gram-negative endotoxins.

Cited by 17 publications

References 28 publications

Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells

Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells

Palmitic Acid and β-Hydroxybutyrate Induce Inflammatory Responses in Bovine Endometrial Cells by Activating Oxidative Stress-Mediated NF-κB Signaling

Effect of a high-fat diet on the rat bladder wall and bioactive action of Brazil nut oil

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