Satureja montana L. essential oil and its antimicrobial activity alone or in combination with gentamicin

Vitanza, Luca; Maccelli, Alessandro; Migliori, Massimiliano; Scazzocchio, F; Comanducci, Antonella; Fornarini, Simonetta; Crestoni, Maria Elisa; Filippi, Antonello; Fraschetti, Caterina; Rinaldi, Federica; Aleandri, Marta; Goldoni, Paola; Conte, Maria Pia; Ammendolia, Maria Grazia; Longhi, Catia

doi:10.1016/j.micpath.2018.11.025

Cited by 53 publications

(43 citation statements)

References 30 publications

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“…The results confirm the SEOs activity against multidrug bacterial strains. According with other studies [24,53,54], as reported in Table 7, MIC and MBC values ranged from 0.39 to 6.25 mg•mL −1 . Commercial SEOT showed the lowest MIC and MBC values towards Gram-positive and Gram-negative bacteria.…”

Section: Antibacterial Activity Of Eos and Nessupporting

confidence: 62%

“…In addition, FA derivatives responsible for specific flavor development [46], including alcoholic (hexenol) and aldehydic (hexanal and octanal) products, were revealed either in SEO3 or in SEO1 and SEO2, respectively. Experiments carried out by APCI-QTRAP MS have allowed to magnify the ionization of less polar compounds and to conduct subsequent CID assays, as in the case of the peak at m/z 155 assigned to protonated terpenoids [C 10 H 19 O] + ( Figure S2, Table S1), whose dissociation pattern is compatible with borneol and linalool, as previously reported for SEOT [24]. Other metabolites were also characterized by the same procedure, including [limonene + H] + and [limonene aldehyde − H] − as shown in Figure S3A,B, respectively.…”

Section: Ms Metabolite Profilingmentioning

confidence: 65%

“…All SEOs present vitamin D derivatives, like methyl-and dihydroxy-vitamin D3, while SEO1 and SEOT hold the carotenoid zeta-carotene, and retinol (C 20 H 30 O) and retinal (C 20 H 28 O) vitamins, respectively. As reported in a preliminary contribution for SEOT [24], all the samples from wild growing populations SEO1-3 contain: (i) terpenes, like γ-terpinene revealed as [C 10 H 17 ] + in ESI(+) at m/z 137.13245, and related compounds, i.e., p-cymene, detected as [C 10 H 15 ] + in ESI(+) at m/z 135.11693; (ii) terpenoids, including carvacrol, and thymol, found as deprotonated species [C 10 H 14 O-H] − at m/z 149.09717, as shown in the mass spectrum of SEO3 ( Figure S1); (iii) free fatty acids (FAs), including saturated medium-long chain FAs, i.e., myristic (14:0), palmitic (16:0) and stearic (18:0) acids, at m/z 227.20182, 255.23239 and 283.26299, respectively, and monounsaturated FA, i.e., oleic (18:1) acid at m/z 281.24859. In addition, FA derivatives responsible for specific flavor development [46], including alcoholic (hexenol) and aldehydic (hexanal and octanal) products, were revealed either in SEO3 or in SEO1 and SEO2, respectively.…”

Section: Ms Metabolite Profilingmentioning

confidence: 77%

“…Regarding E. coli strains, MIC and MBC values for oil coincided in the range 1.56-3.12 mg•mL −1 . Antimicrobial activity of the members of Satureja genus is well known [3,24,25]. Significant antimicrobial activity of SEO has been previously related to its content in secondary metabolites, including carvacrol, thymol and terpinen-4-ol, probably through membrane damage [19].…”

Section: Antibacterial Activity Of Eos and Nesmentioning

confidence: 99%

“…Several studies reported that EOs, derived from different species of Satureja genus, possess remarkable antibacterial activities against different microorganisms such as Escherichia coli, Listeria spp., and Staphylococcus spp. [19,24,25]. This antimicrobial range and activity are likely dependent on the composition of the essential oil and the origin of the plant.…”

Section: Introductionmentioning

confidence: 99%

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Satureja montana L. Essential Oils: Chemical Profiles/Phytochemical Screening, Antimicrobial Activity and O/W NanoEmulsion Formulations

et al. 2019

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Chemical fingerprints of four different Satureja montana L. essential oils (SEOs) were assayed by an untargeted metabolomics approach based on Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) coupled with either electrospray ionization or atmospheric pressure chemical ionization ion sources. Analysis and relative quantification of the non-polar volatile fraction were conducted by gas chromatography (GC) coupled to MS. FT-ICR MS confirmed significant differences in the polar metabolite composition, while GC-MS analyses confirmed slight fluctuations in the relative amount of major terpenes and terpenoids, known to play a key role in antimicrobial mechanisms. Oil in eater (O/W) nanoemulsions (NEs) composed by SEOs and Tween 20 or Tween 80 were prepared and analyzed in terms of hydrodynamic diameter, ζ-potential and polydispersity index. The results confirm the formation of stable NEs homogeneous in size. Minimum inhibitory and minimum bactericidal concentrations of SEOs were determined towards Gram-positive (Listeria monocytogenes, Staphylococcus aureus, Staphylococcus haemolyticus) and Gram-negative clinical isolates (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Serratia marcescens). Commercial SEO showed strongest antibacterial activity, while SEO 3 was found to be the most active among the lab made extractions. MIC and MBC values ranged from 0.39 to 6.25 mg·mL−1. Furthermore, a SEO structured in NEs formulation was able to preserve and improve antimicrobial activity.

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Section: Antibacterial Activity Of Eos and Nessupporting

confidence: 62%

Section: Ms Metabolite Profilingmentioning

confidence: 65%

Section: Ms Metabolite Profilingmentioning

confidence: 77%

Section: Antibacterial Activity Of Eos and Nesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Satureja montana L. Essential Oils: Chemical Profiles/Phytochemical Screening, Antimicrobial Activity and O/W NanoEmulsion Formulations

et al. 2019

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FT‐ICR‐MS‐based metabolomics: A deep dive into plant metabolism

Maia

Figueiredo

Cordeiro

et al. 2021

Mass Spectrometry Reviews

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Metabolomics involves the identification and quantification of metabolites to unravel the chemical footprints behind cellular regulatory processes and to decipher metabolic networks, opening new insights to understand the correlation between genes and metabolites. In plants, it is estimated the existence of hundreds of thousands of metabolites and the majority is still unknown. Fourier transform ion cyclotron resonance mass spectrometry (FT‐ICR‐MS) is a powerful analytical technique to tackle such challenges. The resolving power and sensitivity of this ultrahigh mass accuracy mass analyzer is such that a complex mixture, such as plant extracts, can be analyzed and thousands of metabolite signals can be detected simultaneously and distinguished based on the naturally abundant elemental isotopes. In this review, FT‐ICR‐MS‐based plant metabolomics studies are described, emphasizing FT‐ICR‐MS increasing applications in plant science through targeted and untargeted approaches, allowing for a better understanding of plant development, responses to biotic and abiotic stresses, and the discovery of new natural nutraceutical compounds. Improved metabolite extraction protocols compatible with FT‐ICR‐MS, metabolite analysis methods and metabolite identification platforms are also explored as well as new in silico approaches. Most recent advances in MS imaging are also discussed.

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From formulation to in vivo model: A comprehensive study of a synergistic relationship between vancomycin, carvacrol, and cuminaldehyde against Enterococcus faecium

Owen

Webb

Green

et al. 2020

Phytotherapy Research

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Vancomycin‐resistant Enterococcus faecium (VRE) has become endemic in healthcare settings, reducing treatment options for enterococcal infections. New antimicrobials for VRE infections are a high priority, but the development of novel antibiotics is time‐consuming and expensive. Essential oils (EOs) synergistically enhance the activity of some existing antibiotics, suggesting that EO–antibiotic combinations could resensitise resistant bacteria and maintain the antibiotic repertoire. The mechanism of resensitisation of bacteria to antibiotics by EOs is relatively understudied. Here, the synergistic interactions between carvacrol (1.98 mM) and cuminaldehyde (4.20 mM) were shown to reestablish susceptibility to vancomycin (0.031 mg/L) in VRE, resulting in bactericidal activity (4.73 log10 CFU/ml reduction). Gene expression profiling, coupled with β‐galactosidase leakage and salt tolerance assays, suggested that cell envelope damage contributes to the synergistic bactericidal effect against VRE. The EO–vancomycin combination was also shown to kill clinical isolates of VRE (2.33–5.25 log10 CFU/ml reduction), and stable resistance did not appear to develop even after multiple passages. The in vivo efficacy of the EO–vancomycin combination was tested in a Galleria mellonella larvae assay; however, no antimicrobial action was observed, indicating that further drug development is required for the EO–vancomycin combination to be clinically useful for treatment of VRE infections.

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Satureja montana L. essential oil and its antimicrobial activity alone or in combination with gentamicin

Cited by 53 publications

References 30 publications

Satureja montana L. Essential Oils: Chemical Profiles/Phytochemical Screening, Antimicrobial Activity and O/W NanoEmulsion Formulations

Satureja montana L. Essential Oils: Chemical Profiles/Phytochemical Screening, Antimicrobial Activity and O/W NanoEmulsion Formulations

FT‐ICR‐MS‐based metabolomics: A deep dive into plant metabolism

From formulation to in vivo model: A comprehensive study of a synergistic relationship between vancomycin, carvacrol, and cuminaldehyde against Enterococcus faecium

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