Sauchinone, a Lignan from Saururus chinensis, Inhibits Staurosporine-Induced Apoptosis in C6 Rat Glioma Cells

Song, Hyun Beom; Kim, Young Choong; Moon, Aree

doi:10.1248/bpb.26.1428

Cited by 22 publications

(15 citation statements)

References 25 publications

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“…Saururus chinensis is a commonly used herbal folk in China and Korea and its extracts have been reported to possess several biological activities including anti-oxidant , anti-cancer , anti-asthmatic (Lee et al, 2006a), anti-inflammatory (Cho et al, 2005), neuroprotective (Song et al, 2003), and immunosuppressive activities (Park et al, 2007). Interestingly, two compounds isolated from S. chinensis-sauchinone and (À)-saucerneol-and its extracts have been recently reported to inhibit osteoclast differentiation and bone resorptive activity of mature osteoclasts suggesting that the extracts of S. chinensis and its phytochemicals could potentially inhibit the differentiation and function of osteoclasts (Han et al, 2007;Shim et al, 2008;Kim et al, 2009b).…”

Section: Journal Of Cellular Physiology a N T I -R E S O R P T I V E mentioning

confidence: 99%

Saurolactam inhibits osteoclast differentiation and stimulates apoptosis of mature osteoclasts

Kim

Ryu

Choi

et al. 2009

Journal Cellular Physiology

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The receptor activator of nuclear factor-kappaB ligand (RANKL) plays a critical role in the differentiation and bone resorptive activity of osteoclasts. Recently, the development of anti-resorptive agents from natural substances has become a subject of interest. Therefore, we evaluated the effects of 222 natural compounds on the RANKL-induced tartrate-resistance acid phosphatase (TRAP; a marker for osteoclast differentiation) activity and multinucleated osteoclast formation in RAW264.7 murine macrophage cells. We found that saurolactam was one of the compounds inhibiting the RANKL-induced osteoclastogenesis; it significantly inhibited the RANKL-induced TRAP activity and formation of multinucleated osteoclasts without any cytotoxicity. Interestingly, saurolactam prevented RANKL-induced activation of MAP kinases and NF-kappaB, and mRNA expression of osteoclast-related genes and transcription factors (c-Fos, Fra-2, and NFATc1). We also observed the inhibitory effect of saurolactam on the differentiation of mouse bone marrow-derived macrophages into osteoclasts. Furthermore, saurolactam inhibited the bone resorptive activity of mature osteoclasts with the induction of apoptotic signaling cascade and the inhibition of survival signaling pathways such as c-Src/PI3K/Akt, Ras/ERK, and JNK/c-Jun. In conclusion, although further studies are needed to determine the precise mechanism and biological efficacy of saurolactam in osteoclast-mediated bone disorders, our results demonstrate that saurolactam potentially inhibits osteoclast differentiation by preventing the activation of MAP kinases and transcription factors that consequently affect the regulation of genes required for osteoclastogenesis, and the bone resorptive activity of mature osteoclasts by inhibiting osteoclast survival-related signaling pathways and triggering the apoptotic signaling cascade.

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Section: Journal Of Cellular Physiology a N T I -R E S O R P T I V E mentioning

confidence: 99%

Saurolactam inhibits osteoclast differentiation and stimulates apoptosis of mature osteoclasts

Kim

Ryu

Choi

et al. 2009

Journal Cellular Physiology

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“…1A). [9][10][11][12][13] We examined the biological effects of these compounds on anti-inflammatory action in dsRNA-stimulated microglia. To assess cytotoxicity, microglia were treated with the two compounds for 24 h, and the level of cell survival was measured using the MTT assay (Fig.…”

Section: Fraxinellone and Sauchinone Inhibit Inos Expression And Nitrmentioning

confidence: 99%

“…Earlier reports show that fraxinellone, the major component of Cortex Dictamni isolated from Chinese medicinal herbs, exerts anti-inflammatory and neuroprotective effects. 9) In addition, sauchinone, a lignin from Saururus chinensis, functions in hepatoprotection and neuroprotection, 10,11) and inhibits lipopolysaccharide (LPS)-induced iNOS expression through blockade of nuclear factor (NF)-kB activation. 12,13) However, the signaling mechanisms involved in the anti-inflammatory actions of these compounds are yet to be clearly clarified.…”

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confidence: 99%

Inhibition of Double-Stranded RNA-Induced Inducible Nitric Oxide Synthase Expression by Fraxinellone and Sauchinone in Murine Microglia

Lee

Won

Yoo

et al. 2009

Biological & Pharmaceutical Bulletin

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“…11) Sauchinone, a biologically active lignan isolated from the roots of S. chinensis, exibits various pharmacological activities, including hepatoprotective, antiinflammatory, cytoprotective and immunosuppressive effects. [12][13][14][15][16] Moreover, several published studies demonstrate the inhibitory effect of sauchinone on LPS-induced expression of iNOS, iNOS-derived nitric oxide, TNF-a and COX-2 in murine macrophages. 12,17) As a part of our ongoing research to identify phytochemicals isolated from natural sources that can induce HO-1 in vitro, 18,19) sauchinone was shown significantly induce the levels of HO-1 in RAW264.7 macrophages.…”

mentioning

confidence: 99%

Sauchinone Suppresses Pro-inflammatory Mediators by Inducing Heme Oxygenase-1 in RAW264.7 Macrophages

Lee

Choi

et al. 2011

Biological & Pharmaceutical Bulletin

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Heme oxygenase-1 (HO-1) is a rate-limiting enzyme in heme catabolism responsible for catalyzing the split of free heme into carbon monoxide (CO), free iron and biliverdin/bilirubin. 1) Recent studies have shown that HO-1 has proven involvment in the resolution of inflammatory responses.2) HO-1 and its enzymatic by-products are the critical regulators of inflammation predominantly targeted against macrophages.3) Studies have shown that the anti-inflammatory action of HO-1 is mediated by inhibition of the production of pro-inflammatory cytokines and chemokines such as tumor necrosis factor-a (TNF-a), interleukin-1b (IL-1b) and interleukin (IL)-6 in activated macrophages. 4,5) Studies indicate that HO-1 and its product carbon monoxide (CO) can suppress the expression of the pro-inflammatory cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), thereby reducing COX-2-drived prostaglandin E 2 (PGE 2 ) and iNOS-derived nitric oxide (NO) production.6,7) Mitogen-activated protein kinases (MAPKs) is one of the most common signaling pathways that serve to coordinate the cellular response to a variety of extracellular stimuli. MAPKs have an important role in cellular processes, such as proliferation, stress responses, apoptosis and immune defense.8) The MAPK activation also modulates several gene and protein expression including the activation of HO-1. 9,10) Saururus chinensis (LOUR.) BAILL. (Saururaceae) has been traditionally used for the treatment of various diseases such as beriberi, pneumonia, urinary disorders, jaundice and gonorrhea. In Korea this plant is a known folk medicine, used for the treatment of edema.11) Sauchinone, a biologically active lignan isolated from the roots of S. chinensis, exibits various pharmacological activities, including hepatoprotective, antiinflammatory, cytoprotective and immunosuppressive effects.12-16) Moreover, several published studies demonstrate the inhibitory effect of sauchinone on LPS-induced expression of iNOS, iNOS-derived nitric oxide, TNF-a and COX-2 in murine macrophages.12,17) As a part of our ongoing research to identify phytochemicals isolated from natural sources that can induce HO-1 in vitro, 18,19) sauchinone was shown significantly induce the levels of HO-1 in RAW264.7 macrophages. In this study, we demonstrate that the effects of sauchinone on lipopolyscharride (LPS)-induced PGE 2 , NO, TNF-a and IL-1b production were partially reversed by the HO-1 inhibitor tin protoporphyrin. We further provided evidence to support the role of HO-1 in mediation of the antiinflammatory effects of sauchinone. Further this study provided evidence that HO-1 is a critical mechanism by which sauchinone exerts anti-inflammatory effects. Korea. Received April 7, 2011; accepted July 8, 2011; published online July 14, 2011 Sauchinone, a biologically active lignan isolated from the roots of Saururus chinensis (LOUR.) BAILL. (Saururaceae), is reported to exert a variety of biological activities, such as hepatoprotective, anti-inflammatory actions and inhibitory effects on...

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Sauchinone, a Lignan from Saururus chinensis, Inhibits Staurosporine-Induced Apoptosis in C6 Rat Glioma Cells

Cited by 22 publications

References 25 publications

Saurolactam inhibits osteoclast differentiation and stimulates apoptosis of mature osteoclasts

Saurolactam inhibits osteoclast differentiation and stimulates apoptosis of mature osteoclasts

Inhibition of Double-Stranded RNA-Induced Inducible Nitric Oxide Synthase Expression by Fraxinellone and Sauchinone in Murine Microglia

Sauchinone Suppresses Pro-inflammatory Mediators by Inducing Heme Oxygenase-1 in RAW264.7 Macrophages

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