The genus Alstonia belonging to the Apocyanaceae family is represented by 44 species distributed worldwide. In India, the taxon is represented by eight species, reported from Andaman & Nicobar Islands, Western Ghats, Eastern Ghats, western Himalaya, and northeastern regions. The present study reported the occurrence of Alstonia sebusii from Pudunamei village, Mao, a new addition to the flora of Manipur. Detailed morphological descriptions and measurements were recorded based on living plant specimens. The plant is locally called ‘Topfiira Koso Pro’ having ethno-medicinal properties and widely been used by local people of the state to treat various ailments. The plant is rare and found to occur at specific location at Pudunamei village.