This paper examines the phonetic realization of rising pre-nuclear pitch accents in Persian. In a first experiment, the alignment of f0 valleys and peaks in pre-nuclear pitch accents was analyzed in controlled speech materials as a function of the syllable structure (open vs. closed) and vowel type (short vs. long) of the accented syllable. The results revealed that in words with antepenultimate stress, both the L and the H tones are anchored to specific segmental landmarks irrespective of syllable structure or vowel type. In particular, the L is consistently aligned with the onset of the accented syllable, and the H is placed with similar consistency in the vicinity of the first post-accentual vowel. In a second experiment, the variability in the timing and scaling of L valleys and H peaks was examined as a function of the proximity of the word boundary and of the following accent. The results revealed that while the alignment of the L was unaffected by changes in stress conditions, H peaks were significantly retracted as the syllable approached the end of the word. However, the proximity of the following accent did not produce a significant effect on H alignment. In addition, no significant differences were found on L and H scaling in different stress or tonal crowding conditions. Overall, the results contribute to a growing body of evidence that in the absence of upcoming prosodic pressure, the alignment of pitch targets is specified relative to segmental positions. A comparison between these findings and empirical findings from other languages reveals fine phonetic differences of segmental anchoring that are less likely to be interpreted in terms of distinct association-based phonological representations, and suggests that some aspects of segmental anchoring need to be explained in terms of continuous language-specific alignment rules.