SAXS imaging reveals optimized osseointegration properties of bioengineered oriented 3D-PLGA/aCaP scaffolds in a critical size bone defect model

Casanova, Elisa A.; Rodríguez-Palomo, Adrián; Stähli, Lisa; Arnke, Kevin; Gröninger, Olivier G.; Generali, Melanie; Neldner, Yvonne; Tiziani, Simon; Domínguez, Ana Pérez; Guizar‐Sicairos, Manuel; Appel, Christian; Nielsen, Leonard C.; Georgiadis, Marios; Weber, Felix; Stark, Wendelin J.; Pape, Hans‐Christoph; Cinelli, Paolo; Liebi, Marianne

doi:10.1016/j.biomaterials.2022.121989

Cited by 12 publications

(15 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These coatings have good biocompatibility and application prospects for bone repair. Elisa et al [97] designed a PLGA three-dimensional fibre scaffold doped with amorphous calcium phosphate by electrospinning technology. The scaffold showed high cell compatibility and osteogenic properties.…”

Section: Calcium Phosphate Coating Induces Osteogenesismentioning

confidence: 99%

See 1 more Smart Citation

Biofunctionalisation strategies of material surface and the inspired biological effects for bone repair

Duan,

Chang,

Zhang

et al. 2024

Biosurface and Biotribology

View full text Add to dashboard Cite

Due to trauma and disease, bone defects endanger the healthy life of human beings. At present, the gold standard for bone defect repair is still autologous bone transplantation and allogeneic bone transplantation. However, its insufficient source, potential disease transmission and immune rejection limit its clinical application. Therefore, the development of bone repair materials plays an important role in promoting bone repair. As the interface between material and tissue, the surface of the material plays an important role in the reaction after implantation, which determines the effectiveness of defect repair treatment. With the development of surface engineering and technology, bone repair materials have developed from biological inertia to biological activity by endowing various biological functions by controlling the composition, topological morphology and structure of the material surface etc. The inspired biofunctionalisation of material surface includes the capacities of inducing osteogenesis, promoting angiogenesis, antibacterial, immune regulation etc., as well as integration of postoperative repair and treatment. The authors review the biofunctionalisation of biomaterial surface and the inspired biological effects for bone repair, mainly including physical and chemical properties of material surface to regulate osteogenesis, and functional strategy of bone repair material surface.

show abstract

Section: Calcium Phosphate Coating Induces Osteogenesismentioning

confidence: 99%

“…High cell compatibility and osteogenic properties [97] Calcium phosphate coatings with tunable microstructure mediated by amorphous metastable phase were fabricated on Ti6Al4V…”

Section: T a B L E 1 (Continued)mentioning

confidence: 99%

Biofunctionalisation strategies of material surface and the inspired biological effects for bone repair

Duan,

Chang,

Zhang

et al. 2024

Biosurface and Biotribology

View full text Add to dashboard Cite

show abstract

“…We have previously performed a number of studies characterizing the biocompatibility and bone regeneration properties of PLGA/aCaP scaffolds in combination with MSCs [29,30,35,[39][40][41]. These scaffolds are designed as bone void fillers with limited tensile properties and are not meant to provide mechanical stability.…”

Section: Scaffold Preparationmentioning

confidence: 99%

“…Despite the promising results obtained with MSCs [35], the potential of differentiation of iPSCs on PLGA/aCaP scaffold was never tested, neither in vitro nor in vivo. In this study, we therefore aimed for the first time at studying the interaction of iPSCs with PLGA/aCaP scaffolds and at assessing the potential of iPSC-seeded and iPSC-ECM-loaded PLGA/aCaP scaffolds for treating critical-size bone defects in an in vivo mouse model.…”

Section: Introductionmentioning

confidence: 99%

Murine iPSC-Loaded Scaffold Grafts Improve Bone Regeneration in Critical-Size Bone Defects

Kessler,

Arnke,

Eggerschwiler

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

In certain situations, bones do not heal completely after fracturing. One of these situations is a critical-size bone defect where the bone cannot heal spontaneously. In such a case, complex fracture treatment over a long period of time is required, which carries a relevant risk of complications. The common methods used, such as autologous and allogeneic grafts, do not always lead to successful treatment results. Current approaches to increasing bone formation to bridge the gap include the application of stem cells on the fracture side. While most studies investigated the use of mesenchymal stromal cells, less evidence exists about induced pluripotent stem cells (iPSC). In this study, we investigated the potential of mouse iPSC-loaded scaffolds and decellularized scaffolds containing extracellular matrix from iPSCs for treating critical-size bone defects in a mouse model. In vitro differentiation followed by Alizarin Red staining and quantitative reverse transcription polymerase chain reaction confirmed the osteogenic differentiation potential of the iPSCs lines. Subsequently, an in vivo trial using a mouse model (n = 12) for critical-size bone defect was conducted, in which a PLGA/aCaP osteoconductive scaffold was transplanted into the bone defect for 9 weeks. Three groups (each n = 4) were defined as (1) osteoconductive scaffold only (control), (2) iPSC-derived extracellular matrix seeded on a scaffold and (3) iPSC seeded on a scaffold. Micro-CT and histological analysis show that iPSCs grafted onto an osteoconductive scaffold followed by induction of osteogenic differentiation resulted in significantly higher bone volume 9 weeks after implantation than an osteoconductive scaffold alone. Transplantation of iPSC-seeded PLGA/aCaP scaffolds may improve bone regeneration in critical-size bone defects in mice.

show abstract

“…Synthetic polymers are of interest because they allow the relatively simple fabrication of scaffolds with open microporous structures and adapted mechanical properties and degradation rates . Poly(lactic- co -glycolic) acid (PLGA) is one of the common biodegradable polymers to produce bone scaffolds , and was approved by the US Food and Drug Administration for clinical use. In addition, PLGA is a linear copolymer with varying ratios of its monomers GA (glycolic acid) and LA (lactic acid), which offers a wide range of degradation rates that can be tailored to specific needs.…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide-Based Composite Hydrogel with Hierarchical Microstructure for Enhanced Vascularization and Skull Regeneration

Lu,

Li,

Wang

et al. 2023

Biomacromolecules

View full text Add to dashboard Cite

Critical-size skull defects caused by trauma, infection, and tumor resection raise great demands for efficient bone substitutes. Herein, a hybrid cross-linked hierarchical microporous hydrogel scaffold (PHCLS) was successfully assembled by a multistep procedure, which involved (i) the preparation of poly(lactic-co-glycolic)/nanohydroxyapatite (PLGA-HAP) porous microspheres, (ii) embedding the spheres in a solution of dopamine-modified hyaluronic acid and collagen I (Col I) and crosslinking via dopamine polyphenols binding to (i) Col I amino groups (via Michael addition) and (ii) PLGA-HAP (via calcium ion chelation). The introduction of PLGA-HAP not only improved the diversity of pore size and pore communication inside the matrix but also greatly enhanced the compressive strength (5.24-fold, 77.5 kPa) and degradation properties to construct a more stable mechanical structure. In particular, the PHCLS (200 mg, nHAP) promoted the proliferation, infiltration, and angiogenic differentiation of bone marrow mesenchymal stem cells in vitro, as well as significant ectopic angiogenesis and mineralization with a storage modulus enhancement of 2.5-fold after 30 days. Meanwhile, the appropriate matrix microenvironment initiated angiogenesis and early osteogenesis by accelerating endogenous stem cell recruitment in situ. Together, the PHCLS allowed substantial skull reconstruction in the rabbit cranial defect model, achieving 85.2% breaking load strength and 84.5% bone volume fractions in comparison to the natural cranium, 12 weeks after implantation. Overall, this study reveals that the hierarchical microporous hydrogel scaffold provides a promising strategy for skull defect treatment.

show abstract

SAXS imaging reveals optimized osseointegration properties of bioengineered oriented 3D-PLGA/aCaP scaffolds in a critical size bone defect model

Cited by 12 publications

References 90 publications

Biofunctionalisation strategies of material surface and the inspired biological effects for bone repair

Biofunctionalisation strategies of material surface and the inspired biological effects for bone repair

Murine iPSC-Loaded Scaffold Grafts Improve Bone Regeneration in Critical-Size Bone Defects

Polysaccharide-Based Composite Hydrogel with Hierarchical Microstructure for Enhanced Vascularization and Skull Regeneration

Contact Info

Product

Resources

About