SbNAC2 enhances abiotic stress tolerance by upregulating ROS scavenging activities and inducing stress-response genes in sorghum

Jin, Xueying; Long, Yun; Xiong, Shiqi; Yang, Zhen; Chen, Wei; Hawar, Amangul; Chi, Xiaoyu; Chen, Yaxin; Luo, Huilian; Qi, Jinliang; Lü, Guihua; Dai, Lingyan; Yang, Yonghua; Sun, Bo

doi:10.1016/j.envexpbot.2021.104664

Cited by 12 publications

(2 citation statements)

References 73 publications

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“…The reaction system was composed of 4.5 μL of cDNA, 7.5 μL of SYBR mix, 0.3 μL of each primer, and 15 μL of ddH 2 O. Thermal cycling included 40 cycles at 95°C for 5 min, 95°C for 10 s, and 58°C for 30 s, and one cycle at 95°C for 10 s, 58°C for 60 s, and 95°C for 10 s. Three biological replicates were performed, and SbEIF4a was used as an internal reference gene ( Yang et al, 2020 ; Du et al, 2021 ; Jin et al, 2021 ). RT-qPCR data were analyzed using the 2 –Δ Δ Ct method ( Pfaffl, 2001 ), and Duncan’s new multiple range test (SPSS software) was used for significance analysis.…”

Section: Methodsmentioning

confidence: 99%

Identification, characterization, and expression profiling of the putative U-box E3 ubiquitin ligase gene family in Sorghum bicolor

Fang

Yang

et al. 2022

Front. Microbiol.

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The U-box family is one of the main E3 ubiquitin ligase families in plants. The U-box family has been characterized in several species. However, genome-wide gene identification and expression profiling of the U-box family in response to abiotic stress in Sorghum bicolor remain unclear. In this study, we broadly identified 68 U-box genes in the sorghum genome, including 2 CHIP genes, and 1 typical UFD2 (Ub fusion degradation 2) gene. The U-box gene family was divided into eight subclasses based on homology and conserved domain characteristics. Evolutionary analysis identified 14, 66, and 82 U-box collinear gene pairs in sorghum compared with arabidopsis, rice, and maize, respectively, and a unique tandem repeat pair (SbPUB26/SbPUB27) is present in the sorghum genome. Gene Ontology (GO) enrichment analysis showed that U-box proteins were mainly related to ubiquitination and modification, and various stress responses. Comprehensive analysis of promoters, expression profiling, and gene co-regulation networks also revealed that many sorghum U-box genes may be correlated with multiple stress responses. In summary, our results showed that sorghum contains 68 U-box genes, which may be involved in multiple abiotic stress responses. The findings will support future gene functional studies related to ubiquitination in sorghum.

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Section: Methodsmentioning

confidence: 99%

Identification, characterization, and expression profiling of the putative U-box E3 ubiquitin ligase gene family in Sorghum bicolor

Fang

Yang

et al. 2022

Front. Microbiol.

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“…Hongke4 and the cold-sensitive cv. SC407 uncovered not only classic cold-inducible genes—e.g., CBL-interacting serine/threonine-protein kinase ( CIPK16 ; Sb02g024770) and stress-activated protein kinase-3 ( SAPK3 ; Sb01g028760)—but also important TFs warranting further functional studies ( MYB62 ; Sb04g026210, NAC1 ; Sb01g003710, WRKY55 ; Sb02g011050, WRKY51 ; Sb03g003360 and WRKY33 ; Sb03g038510) ( Jin et al., 2021 ; Zhou et al., 2022b). These cold-induced genes enriched metabolic functions, namely phenylpropanoid synthesis, carbon metabolism, amino acid biosynthesis, and starch and sucrose metabolism, thus indicating extensive metabolic reprogramming occurs under cold stress.…”

Section: Multi-omic Research Advances In High- and Low-temperature St...mentioning

confidence: 99%

Current advances in the molecular regulation of abiotic stress tolerance in sorghum via transcriptomic, proteomic, and metabolomic approaches

et al. 2023

Front. Plant Sci.

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Sorghum (Sorghum bicolor L. Moench), a monocot C4 crop, is an important staple crop for many countries in arid and semi-arid regions worldwide. Because sorghum has outstanding tolerance and adaptability to a variety of abiotic stresses, including drought, salt, and alkaline, and heavy metal stressors, it is valuable research material for better understanding the molecular mechanisms of stress tolerance in crops and for mining new genes for their genetic improvement of abiotic stress tolerance. Here, we compile recent progress achieved using physiological, transcriptome, proteome, and metabolome approaches; discuss the similarities and differences in how sorghum responds to differing stresses; and summarize the candidate genes involved in the process of responding to and regulating abiotic stresses. More importantly, we exemplify the differences between combined stresses and a single stress, emphasizing the necessity to strengthen future studies regarding the molecular responses and mechanisms of combined abiotic stresses, which has greater practical significance for food security. Our review lays a foundation for future functional studies of stress-tolerance-related genes and provides new insights into the molecular breeding of stress-tolerant sorghum genotypes, as well as listing a catalog of candidate genes for improving the stress tolerance for other key monocot crops, such as maize, rice, and sugarcane.

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SbMYC2 mediates jasmonic acid signaling to improve drought tolerance via directly activating SbGR1 in sorghum

Wang,

Long,

Jin

et al. 2024

Theor Appl Genet

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SbNAC2 enhances abiotic stress tolerance by upregulating ROS scavenging activities and inducing stress-response genes in sorghum

Cited by 12 publications

References 73 publications

Identification, characterization, and expression profiling of the putative U-box E3 ubiquitin ligase gene family in Sorghum bicolor

Identification, characterization, and expression profiling of the putative U-box E3 ubiquitin ligase gene family in Sorghum bicolor

Current advances in the molecular regulation of abiotic stress tolerance in sorghum via transcriptomic, proteomic, and metabolomic approaches

SbMYC2 mediates jasmonic acid signaling to improve drought tolerance via directly activating SbGR1 in sorghum

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