Scaffold‐Free Bio‐3D Printing Using Spheroids as “Bio‐Inks” for Tissue (Re‐)Construction and Drug Response Tests

Murata, Daiki; Arai, Kohei; Nakayama, Koichi

doi:10.1002/adhm.201901831

Cited by 81 publications

(43 citation statements)

References 113 publications

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“…Combination of scaffold-free method and 3D printing technology has also been reported. Cell spheroids were placed and stacked on needles by Kenzan bioprinting method [ 187 ], and scaffold-free structures were fabricated following cell culture and needle removal. It is feasible to prepare simple vascular grafts by this method.…”

Section: Future Perspectives and Conclusionmentioning

confidence: 99%

3D printing of tissue engineering scaffolds: a focus on vascular regeneration

et al. 2021

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Section: Future Perspectives and Conclusionmentioning

confidence: 99%

3D printing of tissue engineering scaffolds: a focus on vascular regeneration

et al. 2021

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“…Alternatively, pre-formed spheroids can also be assembled in 3D using arrays of tiny needles, a procedure known as the Kenzan method. Upon deposition, adjacent tissue spheroids undergo self-assembly forming larger tissue-engineered constructs ( Murata et al, 2020 ).…”

Section: Biofabrication Vs Organ-on-a-chip: Characteristic Advantages Limitations and Challengesmentioning

confidence: 99%

Tackling Current Biomedical Challenges With Frontier Biofabrication and Organ-On-A-Chip Technologies

Celikkin

Presutti

Maiullari

et al. 2021

Front. Bioeng. Biotechnol.

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In the last decades, biomedical research has significantly boomed in the academia and industrial sectors, and it is expected to continue to grow at a rapid pace in the future. An in-depth analysis of such growth is not trivial, given the intrinsic multidisciplinary nature of biomedical research. Nevertheless, technological advances are among the main factors which have enabled such progress. In this review, we discuss the contribution of two state-of-the-art technologies–namely biofabrication and organ-on-a-chip–in a selection of biomedical research areas. We start by providing an overview of these technologies and their capacities in fabricating advanced in vitro tissue/organ models. We then analyze their impact on addressing a range of current biomedical challenges. Ultimately, we speculate about their future developments by integrating these technologies with other cutting-edge research fields such as artificial intelligence and big data analysis.

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“…The Kenzan bioprinting method provides a high-resolution biofabrication process, facilitating the fusion of spheroids into larger tissue constructions in a needle matrix removed after spheroid fusion. This method is used in the Bio-3D Regenova Printer marketed by Cyfuse Biomedical ( Moldovan, 2018 ; Murata et al, 2020 ).…”

Section: D Bioprintingmentioning

confidence: 99%

Recapitulating Tumorigenesis in vitro: Opportunities and Challenges of 3D Bioprinting

Kronemberger

Miranda

Tavares

et al. 2021

Front. Bioeng. Biotechnol.

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Cancer is considered one of the most predominant diseases in the world and one of the principal causes of mortality per year. The cellular and molecular mechanisms involved in the development and establishment of solid tumors can be defined as tumorigenesis. Recent technological advances in the 3D cell culture field have enabled the recapitulation of tumorigenesis in vitro, including the complexity of stromal microenvironment. The establishment of these 3D solid tumor models has a crucial role in personalized medicine and drug discovery. Recently, spheroids and organoids are being largely explored as 3D solid tumor models for recreating tumorigenesis in vitro. In spheroids, the solid tumor can be recreated from cancer cells, cancer stem cells, stromal and immune cell lineages. Organoids must be derived from tumor biopsies, including cancer and cancer stem cells. Both models are considered as a suitable model for drug assessment and high-throughput screening. The main advantages of 3D bioprinting are its ability to engineer complex and controllable 3D tissue models in a higher resolution. Although 3D bioprinting represents a promising technology, main challenges need to be addressed to improve the results in cancer research. The aim of this review is to explore (1) the principal cell components and extracellular matrix composition of solid tumor microenvironment; (2) the recapitulation of tumorigenesis in vitro using spheroids and organoids as 3D culture models; and (3) the opportunities, challenges, and applications of 3D bioprinting in this area.

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Scaffold‐Free Bio‐3D Printing Using Spheroids as “Bio‐Inks” for Tissue (Re‐)Construction and Drug Response Tests

Cited by 81 publications

References 113 publications

3D printing of tissue engineering scaffolds: a focus on vascular regeneration

3D printing of tissue engineering scaffolds: a focus on vascular regeneration

Tackling Current Biomedical Challenges With Frontier Biofabrication and Organ-On-A-Chip Technologies

Recapitulating Tumorigenesis in vitro: Opportunities and Challenges of 3D Bioprinting

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