Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly

Parfenov, Vladislav A.; Koudan, Elizaveta V.; Буланова, Елена А.; Каралкин, П. А.; Pereira, Frederico D. A. S.; Norkin, N. E.; Knyazeva, Alisa D; Gryadunova, A. A.; Петров, О. Ф.; Vasiliev, M. M.; Myasnikov, M. I.; Chernikov, Valery P.; Kasyanov, Vladimir; Marchenkov, A. Yu.; Brakke, Kenneth A.; Khesuani, Yusef D.; Demirci, Utkan

doi:10.1088/1758-5090/aac900

Cited by 75 publications

(72 citation statements)

References 59 publications

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“…In our experiments the employed high magnetic field served as some sort of 'temporal and removable support', which fits very well to the classic definition of a scaffold in tissue engineering [36]. To escape potential confusion, we previously suggested use of the new designation 'scaffield' for defining temporal and removable support provided by any type of physical fields, including the high magnetic field employed in this study [11]. We also suggested defining this type of rapid biofabrication based on using scaffield 'formative biofabrication', which is conceptually different from conventional 'layer-bylayer' additive biofabrication and 3D bioprinting.…”

Section: Discussionsupporting

confidence: 65%

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Scaffold-free and label-free biofabrication technology using levitational assembly in a high magnetic field

et al. 2020

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Section: Discussionsupporting

confidence: 65%

“…2.7. Molecular dynamics simulation of the polystyrene beads and tissue spheroids in the magnetic field of the Bitter magnet The molecular dynamics (MD) modeling was carried out as described previously [11,[21][22][23]. We assumed that all particles in MD calculations were spherical, with the same size and mass m p .…”

Section: Magnetic Experimental Setupmentioning

confidence: 99%

Scaffold-free and label-free biofabrication technology using levitational assembly in a high magnetic field

et al. 2020

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“…The main goal of the present study was to prove the possibility of using the formative approach based on magnetic levitation assembly of the α-TCP particles with subsequent recrystallization into OCP considered as most favorable type of CP for bone remodeling to form 3D scaffold for bone tissue engineering 24,25 . The technology of magnetic levitation has been demonstrated previously in the number of innovative studies 18,22 . The authors have used various objects for magnetic levitation such as polystyrene beads, cells, hydrogel blocks, tissue spheroids etc.…”

Section: Discussionmentioning

confidence: 99%

“…Gadolinium-based (Gd 3+ -based) pharmaceuticals, commonly used via MagLev approach, are FDA approved for clinical use as MRI contrast agents and consequently comprise non-toxic agents The magnetic levitational assembly has been proposed recently as a novel way to fabricate biomaterials, scaffolds for tissue engineering [18][19][20][21] . The magnetic fabrication of viable chondrospheres-based scaffolds via levitation has been demonstrated in our previous study 22 .…”

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Fabrication of calcium phosphate 3D scaffolds for bone repair using magnetic levitational assembly

Parfenov

Mironov

Koudan

et al. 2020

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The calcium phosphate particles can be used as building blocks for fabrication of 3D scaffolds intended for bone tissue engineering. This work presents for the first time a rapid creation of 3D scaffolds using magnetic levitation of calcium phosphate particles. Namely, tricalcium phosphate particles of equal size and certain porosity are used, which undergo the process of recrystallization after magnetic levitational assembly of the scaffold to ensure stitching of the scaffold. Label-free levitational assembly is achieved by using a custom-designed magnetic system in the presence of gadolinium salts, which allows the levitation of calcium phosphate particles. Chemical transformation of tricalcium-to octacalcium phosphate under the condition of magnetic levitation in non-homogeneous magnetic field is also demonstrated. This approach allows obtaining rapidly the octacalcium phosphate phase in the final 3D product, which is biocompatible.

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“…The ability to trap and manipulate small objects in three dimensions—using either optical, magnetic, acoustic, or dielectrophoretic fields or microfluidic flows—has enabled applications ranging from atom‐by‐atom assembly, to Bose–Einstein condensation, to the manipulation of biomolecules, colloids and cells, to artificial insemination, and more . These methods differ in and are limited by the types and sizes of objects that can be manipulated—in particular, few can flexibly address and assemble larger nonmagnetic particles, from a tens of micrometers to millimeters, such as the components of mechanical systems or optical devices, or tissue‐like assemblies . Here, we describe trapping in this regime of sizes enabled by vortex flows created within a rotating frame of reference.…”

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Dynamic Assembly of Small Parts in Vortex–Vortex Traps Established within a Rotating Fluid

et al. 2019

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trapping. Using this arrangement, we demonstrate trapping and dynamic assembly of various particle clusters, cages, and interlocked architectures, as well as jammed colloidal monoliths or colloidal formations on gas bubbles. In these experiments, the shape of the trapping region-and, consequently, the morphology of the assembling structures-can be controlled by adjusting the strengths of one or both vortices and/or the system's global angular velocity, and can switch from a stable equilibrium point to a limit cycle via the so-called supercritical Hopf bifurcation. These findings illustrate how new modalities of dynamic self-assembly [26] and 3D manipulation become possible upon transition from static to noninertial, rotating frames of reference.When a lighter particle of volume V and density ρ P is immersed in a rotating fluid of density ρ L > ρ P , it experiences not only an upward-directed buoyant force F B = (ρ P − ρ L )Vg, but also a centripetal force directed toward the axis of tube's rotation, F C (r) = −(ρ L −ρ P )Vrω 2 , where ω is fluid's angular velocity and vector r specifies particle's radial position (Figure 1a). In other words, the rotation of the tube imposes a confining harmonic potential, E(r) = (ρ L − ρ P )Vr 2 ω 2 /2. As we have shown previously, [27] for polymer beads inside tubes ≈1 cm in diameter, filled with various aqueous salt solutions and rotating at few thousand rpm, centrifugal acceleration is on the order of 10g (allowing us to neglect buoyancy effects in the subsequent discussion), and the beads localize along the fluid's axis of rotation. On the other hand, radially directed forces do not displace the beads along the tube's rotation axis and cannot, by themselves, create a stable trapping region in the axial direction.To enable such lateral confinement, we designed a system (cf. Section S1, Supporting Information) in which two aluminum disks (radius 9.9 mm and thickness 16 mm) are fitted inside and near the two ends of the liquid-filled, rotating tube ≈1 cm in diameter. A permanent neodymium bar magnet (BX088-N52 from KJ Magnetics; w = h = 12.5 mm and l = 25.4 mm, magnetization along the w dimension = 588 mT) is placed at a distance d away from each disk (Figure 1b). The role of the magnets is to act as eddy-current brakes and slow down the rotation of the aluminum disks with respect to the rotation of the liquid-filled tube. Indeed, as the distance d between the disks and the magnets decreases, so does the disks' angular velocity, ω 1 . For a given d, ω 1 increases with but is always smaller than the angular velocity of the tube, ω (Figure 1c). This slowed-down rotation of the disks gives rise to Stable, purely fluidic particle traps established by vortex flows induced within a rotating fluid are described. The traps can manipulate various types of small parts, dynamically assembling them into high-symmetry clusters, cages, interlocked architectures, jammed colloidal monoliths, or colloidal formations on gas bubbles. The strength and the shape of the trapping region can be control...

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Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly

Cited by 75 publications

References 59 publications

Scaffold-free and label-free biofabrication technology using levitational assembly in a high magnetic field

Scaffold-free and label-free biofabrication technology using levitational assembly in a high magnetic field

Fabrication of calcium phosphate 3D scaffolds for bone repair using magnetic levitational assembly

Dynamic Assembly of Small Parts in Vortex–Vortex Traps Established within a Rotating Fluid

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