Scaffold-free parathyroid tissue engineering using tonsil-derived mesenchymal stem cells

Park, Yoon Shin; Hwang, Ji-Young; Jun, Yesl; Jin, Yoon; Kim, Gyungah; Kim, Ha Yeong; Lee, Sang Hoon; Jo, Inho

doi:10.1016/j.actbio.2016.03.003

Cited by 33 publications

(30 citation statements)

References 36 publications

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“…The PTX rats receiving these implants also showed higher levels of serum PTH, ionized Ca 2+ , and survival rates compared with those groups receiving no implants or implants containing nondifferentiated control TMSCs . Additionally, the subcutaneous injection of TMSCs differentiated into PTH‐releasing cells in the form of a scaffold‐free 3D spheroid showed similar results as it was observed from the studies with scaffolding materials . More recently, it was shown that the intramuscular injection of the PTH‐releasing cells derived from TMSCs fabricated with autologous plasma to the rat dorsum resulted in elevation of serum PTH and chromogranin A in PTX rats .…”

Section: Tonsil‐derived Mscs (Tmscs)supporting

confidence: 62%

Application of Tonsil-Derived Mesenchymal Stem Cells in Tissue Regeneration: Concise Review

Choi

Kim

et al. 2019

Stem Cells

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Since the discovery of stem cells and multipotency characteristics of mesenchymal stem cells (MSCs), there has been tremendous development in regenerative medicine. MSCs derived from bone marrow have been widely used in various research applications, yet there are limitations such as invasiveness of obtaining samples, low yield and proliferation rate, and questions regarding their practicality in clinical applications. Some have suggested that MSCs from other sources, specifically those derived from palatine tonsil tissues, that is, tonsil-derived MSCs (TMSCs), could be considered as a new potential therapeutic tool in regenerative medicine due to their superior proliferation rate and differentiation capabilities with low immunogenicity and ease of obtaining. Several studies have determined that TMSCs have differentiation potential not only into the mesodermal lineage but also into the endodermal as well as ectodermal lineages, expanding their potential usage and placing them as an appealing option to consider for future studies in regenerative medicine. In this review, the differentiation capacities of TMSCs and their therapeutic competencies from past studies are addressed. STEM CELLS 2019;37:1252-1260 SIGNIFICANCE STATEMENTMesenchymal stem cells (MSCs) are considered as a great candidate for tissue engineering in regenerative medicine. Tonsil-derived MSCs (TMSCs) could be an attractive option for clinical applications because of their noninvasiveness of tissue collection, relatively high proliferation rate, and low allogenicity. This review addresses potential differentiation capabilities of TMSCs into mesodermal, endodermal, and ectodermal lineages reported from previous in vitro and in vivo studies as well as their potential applications for treating various human diseases.

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Section: Tonsil‐derived Mscs (Tmscs)supporting

confidence: 62%

Application of Tonsil-Derived Mesenchymal Stem Cells in Tissue Regeneration: Concise Review

Choi

Kim

et al. 2019

Stem Cells

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“…Grafts made out of parathyroid tissue encapsulated into microspheres in an attempt to prevent graft rejection showed survival between 12 weeks [ 29 ] and 20 months [ 30 ]. Scaffold-free parathyroid tissue spheroids using differentiated tonsil-derived mesenchymal stem cells to restore in vivo parathyroid cell functions remained effective over 3 months [ 31 ]. It appears therefore that the limited experience reached to date has failed to identify a transplantation technique that could provide long-term clinical effectiveness.…”

Section: Discussionmentioning

confidence: 99%

Patients’ views about parathyroid transplantation for post-thyroidectomy hypoparathyroidism

Schmeißer

Mihai

2018

Langenbecks Arch Surg

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BackgroundPermanent hypoparathyroidism (hypoPT) represents the most common postoperative complication associated with total thyroidectomy. Current treatment relies on high-dose calcium and/or vitamin D supplementation, but often this is insufficient and some patients remain symptomatic. Parathyroid allotransplantation is a new therapeutic option described recently in the literature. This study aims to investigate the patients’ acceptability of parathyroid transplantation as a potential new treatment for hypoPT.MethodOnline survey of members of HypoParaUK, a support group for individuals affected by hypoPT.ResultsResponses were received from 252 hypoPT patients. Majority declared to experience severe symptoms despite regular medical treatment. On a severity scale of 0–5, symptoms that were most troublesome were fatigue (3.8), low sense of well-being (3.5), and numbness/tingling (2.9). On a scale of 0–10, on average, their current quality of life (QoL) was 5 ± 3 and they expected this would improve to 7 ± 2 with correction of their hypoPT. Forty-four percent of patients were extremely interested in a potential technique involving intramuscular injection of parathyroid cell suspension compared to just 14% who were interested in the more invasive procedure of implantation of a parathyroid allograft into the forearm. The main concerns expressed were related to the possible need for immunosuppressive therapy.ConclusionPatients with severe symptomatic hypoPT seem interested to consider participation in a clinical trial exploring the feasibility and success rate of parathyroid transplantation.Electronic supplementary materialThe online version of this article (10.1007/s00423-018-1693-y) contains supplementary material, which is available to authorized users.

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“…It is well known that when mesenchymal stem cells are cultured in 3D culture system, they promote cell differentiation [ 37 , 38 ]. Most recently, we reported that implantation with self-assembled 3D TMSC spheroids led to favorable effects on survival rates and parathyroid hormone levels in parathyroidectomized rats compared to plain 2D TMSC culture [ 25 ]. Because of the higher levels of CD31, an endothelial cell marker, and N-cadherin, a protein responsible for cell-to-cell interaction, in the skin area of implanted TMSC spheroids from our previous study, we addressed whether the improved efficiency by spheroid implantation was attributable in part to increased TMSC viability in host tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, these cells can easily differentiate into various types of cells such as tenocyte, muscle, and peripheral nerve [ 21 – 23 ]. Interestingly, TMSC can also differentiate into endodermal origin cells, such as parathyroid cells [ 24 , 25 ]. In addition to those distinct characteristics, TMSC exhibit immune regulatory activity like other MSC.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic potential of tonsil-derived mesenchymal stem cells in dextran sulfate sodium-induced experimental murine colitis

Song

Lee

et al. 2017

PLoS ONE

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The therapeutic potential of tonsil-derived mesenchymal stem cells (TMSC) prepared from human tonsillar tissue has been studied in animal models for several diseases such as hepatic injury, hypoparathyroidism, diabetes and muscle dystrophy. In this study, we examined the therapeutic effects of TMSC in a dextran sulfate sodium (DSS)-induced colitis model. TMSC were injected in DSS-induced colitis mice via intraperitoneal injection twice (TMSC[x2]) or four times (TMSC[x4]). Control mice were injected with either phosphate-buffered saline or human embryonic kidney 293 cells. Body weight, stool condition and disease activity index (DAI) were examined daily. Colon length, histologic grading, and mRNA expression of pro-inflammatory cytokines, interleukin 1β (IL-1β), IL-6, IL-17 and tumor necrosis factor α, and anti-inflammatory cytokines, IL-10, IL-11 and IL-13, were also measured. Our results showed a significant improvement in survival rates and body weight gain in colitis mice injected with TMSC[x2] or TMSC[x4]. Injection with TMSC also significantly decreased DAI scores throughout the experimental period; at the end of experiment, almost complete reversal of DAI scores to normal was found in colitis mice treated with TMSC[x4]. Colon length was also significantly recovered in colitis mice treated with TMSC[x4]. However, histopathological alterations induced by DSS treatment were not apparently improved by injection with TMSC. Finally, treatment with TMSC[x4] significantly reversed the mRNA levels of IL-1β and IL-6, although expression of all pro-inflammatory cytokines tested was induced in colitis mice. Under our experimental conditions, however, no apparent alterations in the mRNA levels of all the anti-inflammatory cytokines tested were found. In conclusion, our findings demonstrate that multiple injections with TMSC produced a therapeutic effect in a mouse model of DSS-induced colitis.

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Scaffold-free parathyroid tissue engineering using tonsil-derived mesenchymal stem cells

Cited by 33 publications

References 36 publications

Application of Tonsil-Derived Mesenchymal Stem Cells in Tissue Regeneration: Concise Review

Application of Tonsil-Derived Mesenchymal Stem Cells in Tissue Regeneration: Concise Review

Patients’ views about parathyroid transplantation for post-thyroidectomy hypoparathyroidism

Therapeutic potential of tonsil-derived mesenchymal stem cells in dextran sulfate sodium-induced experimental murine colitis

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