Scaffold implantation in the omentum majus of rabbits for new bone formation

Birkenfeld, Falk; Sengebusch, André; Völschow, Chiara; Möller, Björn; Naujokat, Hendrik; Wiltfang, Jens

doi:10.1016/j.jcms.2019.04.002

Cited by 5 publications

(2 citation statements)

References 42 publications

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“…Once the formalin was removed, the cassettes containing the samples were placed in a tissue processor (Thermo Scientific Shandon Pathcenter, Fisher Scientific, USA) ( Bemenderfer et al ., 2014 ), where tissue dehydration was performed with increasing concentrations of ethanol (60%, 80%, and 96% alcohol) and isopropyl alcohol ( Baskin, 2014 ). Using an embedding center (Tes 99 Paraffin Embedding Center, Medite, Germany) ( Birkenfeld et al ., 2019 ), samples were cleared with chloroform and flushed with xylene, then embedded in paraffin.…”

Section: Methodsmentioning

confidence: 99%

Histomorphometry and μCT scan analysis of osteoporosis in spayed female dogs

Kostenko¹,

ius²,

Maknickas³

2023

Open Vet J

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Background: Both humans and small animals suffer from similar metabolic and structural diseases that impact the musculoskeletal system; however, instead of studying animal disease in its own right, animals are more often used as models for research into various human ailments, such as osteoporosis. There are few studies indicating that animals may suffer from osteoporosis, which raises the question of why small animals, which we believe to be equally susceptible, receive so little attention. With this research, we hope to draw the attention of researchers to the fact that the examination of animals for this disease is just as important as the examination of humans; human osteoporosis research receives a great deal of attention, while animals and their health are neglected. Aims: We aimed to analyse the bone volume fraction (BV/TV) and thickness of first (L1) and second (L2) lumbar vertebrae samples from five cadavers using histomorphometric analysis. In addition, we aimed to investigate one cadaver using microcomputed tomography (micro-CT) imaging. Methods: The L1 and L2 vertebrae from five dog carcasses were used to evaluate the BV/TV and the trabecular thicknesses. We used precise sampling criteria, and also developed a methodological approach to the study of the vertebrae. Using semi-automated methods, we performed histomorphometric analysis and microcomputed tomography data analysis. Results: We used five dog cadavers in this research. During the histomorphometry study, we observed that the lowest L1 BV/TV ratio was 7.88% and the highest was 23.08%. The L2 vertebrae BV/TV ranged from 11.58 to 23.7%. The L1 and L2 lumbar trabeculae thicknesses were also measured. L1's smallest trabecula was 17.34 microns and its largest was 31.88. The L2 vertebrae trabecula thickness was 18.76 to 30.75 microns. BV/TV and trabecular thickness were positively correlated (and vice versa). The two-tailed P value was less than 0.00001. This difference is statistically significant. After microcomputed tomography analysis, we discovered regions in the vertebral body with low porosity; these cavities are usually filled with connective tissue. The bone tissue in these areas is more vulnerable, meaning fracture risk has increased. Conclusions: Animals should not just be considered as models for osteoporosis in humans, but also as potential patients. A single test, such as histomorphometry, may not be sufficient; more advanced technology, such as microcomputed tomography, is required, since it reveals the pores that make the vertebral column more brittle and susceptible to fracture.

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Section: Methodsmentioning

confidence: 99%

Histomorphometry and μCT scan analysis of osteoporosis in spayed female dogs

Kostenko¹,

ius²,

Maknickas³

2023

Open Vet J

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“…In recent decades, many approaches to bone cultivation and ectopic endocultivation of bone flaps have been developed as an alternative approach ‐ also to reduce the morbidity of existing therapies 4–6 . The reconstruction by endocultivation using 3‐dimensional (3D)‐printed scaffolds in the greater omentum is a very promising approach in defect‐specific bone tissue regeneration 7 .…”

Section: Introductionmentioning

confidence: 99%

Robot‐assisted scaffold implantation and two‐stage flap raising of the greater omentum for reconstruction of the facial skeleton: Description of a novel technique

Naujokat

Wieker

Weitkamp

et al. 2022

Robotics Computer Surgery

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Background Bone regeneration in the greater omentum is a promising strategy in facial skeleton reconstruction. This feasibility‐study aims to perform robot‐assisted scaffold implantation and second‐stage flap raising. Methods Firstly, scaffolds were implanted into the greater omentum using the da Vinci Xi surgical system or conventional technique in five miniature pigs, respectively. After 3 months a free flap including the gastrocolic vascular pedicle was harvested and procedures were evaluated. Results The average operation time was 59.2 min for open surgery and 73.8 min for robot‐assisted surgery. The average incision length of open surgery was 11.6 cm. Both techniques led to successful scaffold implantation without any complication. In all cases the scaffolds were integrated as intended and resulted in bone formation. Conclusions Current study demonstrated that the implantation of scaffolds into the greater omentum and flap harvesting using da Vinci Xi surgical system is a promising minimally‐invasive approach in regenerative surgery.

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