Scaffolds for 3D in vitro culture of neural lineage cells

Murphy, Ashley R.; Laslett, Andrew L.; O'Brien, Carmel; Cameron, Neil R.

doi:10.1016/j.actbio.2017.02.046

Cited by 129 publications

(130 citation statements)

References 233 publications

(211 reference statements)

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“…Depending on the application of the materials, different types of matrix materials have been used to support 3D cell culture, which includes synthetic polymers, natural polymers, natural‐synthetic hybrids, ceramics, glass, and carbon nanotubes . Within these materials, hydrogel scaffolds, both natural and synthetic hydrogels, are a great option for neural tissue engineering since they can easily be modified to mimic the elastic properties of nervous system.…”

Section: Discussionmentioning

confidence: 99%

Three‐Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiation

Günay

Sever

Tekinay

et al. 2017

Biotechnology Journal

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The extracellular matrix (ECM) provides biochemical signals and structural support for cells, and its functional imitation is a fundamental aspect of biomaterial design for regenerative medicine applications. The stimulation of neural differentiation by a laminin protein-derived epitope in two-dimensional (2D) and three-dimensional (3D) environments is investigated. The 3D gel system is found to be superior to its 2D counterpart for the induction of neural differentiation, even in the absence of a crucial biological inducer in nerve growth factor (NGF). In addition, cells cultured in 3D gels exhibits a spherical morphology that is consistent with their form under in vivo conditions. Overall, the present study underlines the impact of bioactivity, dimension, and NGF addition, as well as the cooperative effects thereof, on the neural differentiation of PC-12 cells. These results underline the significance of 3D culture systems in the development of scaffolds that closely replicate in vivo environments for the formation of cellular organoid models in vitro.

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Section: Discussionmentioning

confidence: 99%

Three‐Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiation

Günay

Sever

Tekinay

et al. 2017

Biotechnology Journal

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“…To study cell culture in vitro, several alternatives are presented in the literature that describes how to derive an ECM environment mimicking the in vivo situation (Murphy et al, 2017). The complexity of native ECM is said to be represented best by Matrigel™ (Gelain et al, 2006), although often hydrogels consisting of a single ECM component, such as collagen, are applied (Kleinman and Martin, 2005).…”

Section: Biomaterialsmentioning

confidence: 99%

Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

Groot

Sliedregt

Benthem

et al. 2019

The Anatomical Record

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In recent years, there has been an increased interest in stem cells for the purpose of regenerative medicine to deliver a wide range of therapies to treat many diseases. However, two‐dimensional cultures of stem cells are of limited use when studying the mechanism of pathogenesis of diseases and the feasibility of a treatment. Therefore, research is focusing on the strengths of stem cells in the three‐dimensional (3D) structures mimicking organs, that is, organoids, or organ‐on‐chip, for modeling human biology and disease. As 3D technology advances, it is necessary to know which signals stem cells need to multiply and differentiate into complex structures. This holds especially true for the complex 3D structure of the inner ear. Recent work suggests that although other factors play a role, the extracellular matrix (ECM), including its topography, is crucial to mimic a stem cell niche in vitro and to drive stem cells toward the formation of the tissue of interest. Technological developments have led to the investigation of biomaterials that closely resemble the native ECM. In the fast forward moving research of organoids and organs‐on‐chip, the inner ear has hardly received attention. This review aims to provide an overview, by describing the general context in which cells, matrix and morphogens cooperate in order to build a tissue, to facilitate research in 3D inner ear technology. Anat Rec, 303:408–426, 2020. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.

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“…The crucial aspect of the material selected for these scaffolds is that it allows an interaction between cells and the material that is similar to that in vivo between cells and the extracellular matrix (ECM). Different material properties, including mechanical properties, such as stiffness, material chemistry, availability for binding sites, and porosity, affect this interaction …”

Section: Introductionmentioning

confidence: 99%

“…In 3D neuronal cultures, a lack of tissue mimicry associated with the 3D architecture and degrees of freedom and the immature stage of hPSC‐derived neurons are two main challenges that more careful studies on cell adhesion may help overcome . There are several studies in which biomaterials for human neuronal cells have been screened; however, the best is yet to be discovered. Even though many natural and synthetic hydrogels seem to provide a suitable 3D environment for human neurons, more research is needed to study their relevant properties for neuronal cell cultures.…”

Section: Introductionmentioning

confidence: 99%

Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

Ylä‐Outinen

Harju

Joki

et al. 2019

Macromolecular Bioscience

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There is a clear need for novel in vitro models, especially for neuronal applications. Development of in vitro models is a multiparameter task consisting of cell‐, biomaterial‐, and environment‐related parameters. Here, three different human origin neuronal cell sources are studied and cultured in various hydrogel 3D scaffolds. For the efficient evaluation of complex results, an indexing method for data is developed and used in principal component analysis (PCA). It is found that no single hydrogel is superior to other hydrogels, and collagen I (Col1) and hyaluronan–poly(vinyl alcohol) (HA1‐PVA) gels are combined into an interpenetrating network (IPN) hydrogel. The IPN gel combines cell supportiveness of the collagen gel and stability of the HA1‐PVA gel. Moreover, cell adhesion is studied in particular and it is found that adhesion of neurons differs from that observed for fibroblasts. In conclusion, the HA1‐PVA‐col1 hydrogel is a suitable scaffold for neuronal cells and supports adhesion formation in 3D.

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Scaffolds for 3D in vitro culture of neural lineage cells

Cited by 129 publications

References 233 publications

Three‐Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiation

Three‐Dimensional Laminin Mimetic Peptide Nanofiber Gels for In Vitro Neural Differentiation

Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

Screening of Hydrogels for Human Pluripotent Stem Cell–Derived Neural Cells: Hyaluronan‐Polyvinyl Alcohol‐Collagen‐Based Interpenetrating Polymer Network Provides an Improved Hydrogel Scaffold

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